Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium [0.03%]
Allokutzneria和Kibdelosporangium属特异性次级代谢组学研究
Man-Jing Ma,Wen-Chao Yu,Huai-Ying Sun et al.
Man-Jing Ma et al.
Rare actinomycete genera are highly recognized as a promising source of structurally diverse and bioactive natural products. Among these genera, Allokutzneria and Kibdelosporangium are two phylogenetically closely related and have been repo...
Functional investigation of the SAM-dependent methyltransferase RdmB in anthracycline biosynthesis [0.03%]
丝氨酰tRNA甲基转移酶RdmB对蒽环类抗生素生物合成的功能研究
Moli Sang,Qingyu Yang,Jiawei Guo et al.
Moli Sang et al.
A novel sub-class of S-adenosyl-l-methionine (SAM)-dependent methyltransferases catalyze atypical chemical transformations in the biosynthesis of anthracyclines. Exemplified by RdmB from Streptomyces purpurascens, it was found with 10-decar...
Prerna Suchitan Modi,Abhishek Singh,Awyang Chaturvedi et al.
Prerna Suchitan Modi et al.
Tissue on a chip or organ-on-chip (OOC) is a technology that's dignified to form a transformation in drug discovery through the use of advanced platforms. These are 3D in-vitro cell culture models that mimic micro-environment of human organ...
Enhancing levan biosynthesis by destroying the strongly acidic environment caused by membrane-bound glucose dehydrogenase (mGDH) in Gluconobacter sp. MP2116 [0.03%]
破坏葡萄糖脱氢酶介导的强酸环境以增强葡糖杆菌MP2116次生代谢产物莱万特素的生物合成能力
Junjie Tian,Shumin Wei,Wenxing Liang et al.
Junjie Tian et al.
Levan produced by Gluconobacter spp. has great potential in biotechnological applications. However, Gluconobacter spp. can synthesize organic acids during fermentation, resulting in environmental acidification. Few studies have focused on t...
Biosynthesis of the benzylpyrrolidine precursor in anisomycin by a unique ThDP-dependent enzyme [0.03%]
由一个独特的ThDP依赖性酶催化皱隔孢酸生物合成中的苯基丙氨前体的形成
Yongjian Qiao,Junbo Wang,Dashan Zhang et al.
Yongjian Qiao et al.
Anisomycin (compound 1), a multifunctional pyrrolidine antibiotic, primarily inhibits protein biosynthesis by binding to the ribosome. Upon binding to the ribosome, the para-phenol moiety of anisomycin inserts completely into the hydrophobi...
Systematic metabolic engineering enables highly efficient production of vitamin A in Saccharomyces cerevisiae [0.03%]
系统代谢工程使维生素A在酵母中的高效生产成为可能
Yi Shi,Shuhuan Lu,Xiao Zhou et al.
Yi Shi et al.
Vitamin A is a micronutrient critical for versatile biological functions and has been widely used in the food, cosmetics, pharmaceutical, and nutraceutical industries. Synthetic biology and metabolic engineering enable microbes, especially ...
Engineering artificial cross-species promoters with different transcriptional strengths [0.03%]
工程化的异种人工启动子具有不同的转录强度
Wenjie Zuo,Guobin Yin,Luyao Zhang et al.
Wenjie Zuo et al.
As a fundamental tool in synthetic biology, promoters are pivotal in regulating gene expression, enabling precise genetic control and spurring innovation across diverse biotechnological applications. However, most advances in engineered gen...
Establishment of the CRISPR-Cpf1 gene editing system in Bacillus licheniformis and multiplexed gene knockout [0.03%]
枯草芽孢杆菌中的CRISPR-Cpf1基因编辑系统建立及多基因敲除
Suxin Liu,Fengxu Xiao,Youran Li et al.
Suxin Liu et al.
Bacillus licheniformis is a significant industrial microorganism. Traditional gene editing techniques relying on homologous recombination often exhibit low efficiency due to their reliance on resistance genes. Additionally, the established ...
Integrating chemistry knowledge in large language models via prompt engineering [0.03%]
通过提示工程在大型语言模型中集成化学知识
Hongxuan Liu,Haoyu Yin,Zhiyao Luo et al.
Hongxuan Liu et al.
This paper presents a study on the integration of domain-specific knowledge in prompt engineering to enhance the performance of large language models (LLMs) in scientific domains. The proposed domain-knowledge embedded prompt engineering me...
Biodegradation of combined pollutants of polyethylene terephthalate and phthalate esters by esterase-integrated Pseudomonas sp. JY-Q with surface-co-displayed PETase and MHETase [0.03%]
表面展示PETase和MHETase的酯酶工程菌降解聚对苯二甲酸乙二醇酯及酯类污染物复合污染
Haixia Wang,Jiahong Zhu,Meng Sun et al.
Haixia Wang et al.
The waste pollution problem caused by polyethylene terephthalate (PET) plastics poses a huge threat to the environment and human health. As plasticizers, Phthalate esters (PAEs) are widely used in PET production and become combined pollutan...