Pathway and protein channel engineering of Bacillus subtilis for improved production of desthiobiotin and biotin [0.03%]
枯草芽孢杆菌代谢途径和蛋白通道工程提高 Dest-bio 和生物素的产量
Yue Wu,Guang-Qing Du,Dong-Han Ma et al.
Yue Wu et al.
Biotin (vitamin B7) is a crucial cofactor for various metabolic processes and has significant applications in pharmaceuticals, cosmetics, and animal feed. Bacillus subtilis, a well-studied Gram-positive bacterium, presents a promising host ...
De novo synthesis of 1-phenethylisoquinoline in engineered Escherichia coli [0.03%]
工程菌株大肠杆菌从头合成1-苯乙基异喹啉
Yaping Mao,Jiangming Zhu,Qian Zhang et al.
Yaping Mao et al.
Phenylethylisoquinoline alkaloids (PIAs) are medicinally important natural products derived from the 1-phenylethylisoquinoline precursor. Heterologous production of the PIAs remains challenging due to the incomplete elucidation of biosynthe...
Harnessing the microbial interactions from Apocynum venetum phyllosphere for natural product discovery [0.03%]
利用Apocynum venetum叶片球微生物间的相互作用进行天然产物发现
Wei Huang,Xingzhi Jiao,Lingqi Hua et al.
Wei Huang et al.
Natural products (NPs) afforded by living-beings, especially by microscopic species, represent invaluable and indispensable reservoirs for drug leads in clinical practice. With the rapid advancement in sequencing technology and bioinformati...
Recent advances in the biosynthesis and production optimization of gentamicin: A critical review [0.03%]
卡那霉素生物合成及生产优化研究进展：批判性评述
Feng Xu,Kaihao Hu,Ali Mohsin et al.
Feng Xu et al.
Gentamicin, an aminoglycoside antibiotic, is generated by a few species within the genus Micromonospora and has garnered significant attention due to its broad-spectrum efficacy in combating numerous infectious diseases. Comprising a comple...
Engineering the TetR-family transcriptional regulator XNR_0706 to enhance heterologous spinosad production in Streptomyces albus B4 chassis [0.03%]
工程改造tetr家族转录调控因子XNR_0706以提升链霉菌B4宿主异源生产虫漆蜡素水平
Xingjun Cui,Hao Tang,Wenzong Wang et al.
Xingjun Cui et al.
The TetR family of regulators are an important group of transcription regulators that regulate diverse cellular processes in prokaryotes. In this study, we found that XNR_0706, a TetR family regulator, controlled the expression of XNR_0345,...
Engineered probiotic-mediated intratumoral delivery and controlled release of bacterial collagenase for cancer therapy [0.03%]
工程益生菌介导的瘤内递送及细菌胶原酶可控释放的癌症治疗方法
Hong-Rui Li,Bang-Ce Ye
Hong-Rui Li
Elevated collagen levels within breast tumors are strongly associated with tumor progression and present a barrier to effective therapeutic agent penetration within the tumor microenvironment (TME), leading to poor clinical outcomes. To add...
Engineering transcriptional regulatory networks for improving second-generation fuel ethanol production in Saccharomyces cerevisiae [0.03%]
用于改良酵母生产二代燃料乙醇的工程化转录调控网络研究
Dongming Sun,Longhao Wu,Xiaocong Lu et al.
Dongming Sun et al.
Presently, Saccharomyces cerevisiae demonstrates proficient co-fermentation of glucose and xylose, marking a significant advancement in second-generation fuel ethanol production. However, the presence of high concentrations of inhibitors in...
Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid [0.03%]
系统代谢工程改造圆星酵母高效合成植物激素脱落酸
Mei-Li Sun,Ziyun Zou,Lu Lin et al.
Mei-Li Sun et al.
Abscisic acid (ABA) is an important phytohormone with diverse applications. It currently relies on the fermentation of Botrytis cinerea, which suffers from limited availability of genetic engineering tools. Here, Yarrowia lipolytica was eng...
Ana-Belén Blázquez,Nereida Jiménez de Oya
Ana-Belén Blázquez
Flaviviruses affect the lives of millions of people in endemic regions and also have the potential to impact non-endemic areas. Factors such as climate change, global warming, deforestation, and increased travel and trade are linked to the ...
Enhancing substrate specificity of microbial transglutaminase for precise nanobody labeling [0.03%]
增强微生物转谷氨酰胺酶的底物特异性以实现纳米抗体的精确定量标记
Xinglong Wang,Kangjie Xu,Haoran Fu et al.
Xinglong Wang et al.
Streptomyces mobaraenesis transglutaminase (smTG) can be used for site-specific labeling of proteins with chemical groups. Here, we explored the use of modified smTG for the biosynthesis of nanobody-fluorophore conjugates (NFC). smTG cataly...