Enhancing abscisic acid production in Botrytis cinerea through metabolic engineering based on a constitutive promoter library [0.03%]
基于组成型启动子文库的代谢工程增强葡萄孢盘梗霉赤霉素合成
Ling-Ru Wang,Ji-Zi-Hao Tang,Shu-Ting Zhu et al.
Ling-Ru Wang et al.
Abscisic acid (ABA) is an important plant growth regulator with broad applications in agriculture, forestry, and other fields. Currently, the industrial production of ABA primarily relies on microbial fermentation using Botrytis cinerea, bu...
Metabolic engineering of Glarea lozoyensis for high-level production of pneumocandin B0 [0.03%]
代谢工程改造灰节丛梗孢菌高效产生 pneumocandin B0
Xinyi Zhang,Shu Cheng,Jing Yang et al.
Xinyi Zhang et al.
Pneumocandin B0 (PB0) is a lipohexapeptide synthesized by Glarea lozoyensis and serves as the precursor for the widely used antifungal drug caspofungin acetate (Cancidas®). However, the low titer of PB0 results in fermentation and purifica...
Characterization of Aspergillus oryzae mutant and its application in heterologous lipase expression [0.03%]
Aspergillus或yzae突变体的特性分析及异源表达脂肪酶研究
Qinghua Li,Chen Zhang,Jianghua Li et al.
Qinghua Li et al.
The Aspergillus oryzae expression system has been developed into a chassis for the production of heterologous lipases, attributed to its strong capabilities in protein production and secretion, robust post-translational modifications, and f...
Construction of antibiotic-free riboflavin producer in Escherichia coli by metabolic engineering strategies with a plasmid stabilization system [0.03%]
基于质粒稳定系统的代谢工程策略构建抗生素筛选标记的无痕进化平台及应用研究——以维生素Riboflavin生产菌为模型体系的研究
Xiaoling Zhang,Yanan Li,Kang Wang et al.
Xiaoling Zhang et al.
Riboflavin, an important vitamin utilized in pharmaceutical products and as a feed additive, is mainly produced by metabolically engineered bacterial fermentation. However, the reliance on antibiotics in the production process leads to incr...
Moritz Burghardt,Tamir Tuller
Moritz Burghardt
Transient expression in Tobacco is a popular way to produce recombinant proteins in plants. The design of various expression vectors, delivered into the plant by Agrobacterium, has enabled high production levels of some proteins. To further...
Auxotrophy-based curation improves the consensus genome-scale metabolic model of yeast [0.03%]
基于营养缺陷型的筛选改善了酵母基因组范围代谢模型的共识性
Siyu Han,Ke Wu,Yonghong Wang et al.
Siyu Han et al.
Saccharomyces cerevisiae, a widely utilized model organism, has seen continuous updates to its genome-scale metabolic model (GEM) to enhance the prediction performance for metabolic engineering and systems biology. This study presents an au...
Regulatory significance of terminator: A systematic approach for dissecting terminator-mediated enhancement of upstream mRNA stability [0.03%]
终止子的调控作用：一种解析终止子介导的上游mRNA稳定性的系统方法
Jia-Wei Ren,Jin-Peng Zhang,Zi-Lun Mei et al.
Jia-Wei Ren et al.
The primary function of terminators is to terminate transcription in gene expression. Although some studies have suggested that terminators also contribute positively to upstream gene expression, the extent and underlying mechanism of this ...
Li Liang,Xu Shan-Shan,Jiang Yan-Jun
Li Liang
Ergothioneine (ERG), a rare natural thio-histidine derivative with potent antioxidant properties and diverse biological functions, is widely utilized in food processing, cosmetics, pharmaceuticals, and nutritional supplements. Current biopr...
Semi-rational design and modification of phosphoketolase to improve the yield of tyrosol in Saccharomyces cerevisiae [0.03%]
半理性设计和改造磷酸酮醇酶以提高酿酒酵母中酪醇产量
Na Song,Huili Xia,Yaoru Xie et al.
Na Song et al.
Tyrosol is an important component of pharmaceuticals, nutraceuticals, and cosmetics, and their biosynthetic pathways are currently a hot research topic. d-Erythrose 4-phosphate is a key precursor for the biosynthesis of tyrosol in Saccharom...
Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology [0.03%]
基于合成生物学的微生物菌种多样性驱动的异源代谢产物生物合成性能改善研究
Yanting Cao,Jianghua Li,Long Liu et al.
Yanting Cao et al.
Metabolic engineering-driven microbial cell factories have made great progress in the efficient bioproduction of biochemical and recombinant proteins. However, the low efficiency and robustness of microbial cell factories limit their indust...