CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris [0.03%]
通过优化Pichia pastoris中的guide RNA处理策略实现基于CRISPR-Cas9的一站式多重基因组编辑
Kaidi Chen,Gulikezi Maimaitirexiati,Qiannan Zhang et al.
Kaidi Chen et al.
The important methylotrophic yeast Pichia pastoris has been utilized for the production of a variety of heterologous recombinant proteins and has great potential for use in the production of value-added compounds using methanol as a substra...
Polyhydroxyalkanoate production during electroactive biofilm formation and stabilization in wetland microbial fuel cells for petroleum hydrocarbon bioconversion [0.03%]
湿地微生物燃料电池在石油烃生物转化过程中好氧生物膜形成及稳定期间聚羟基烷酸酯的生成
Lanmei Zhao,Mengxue Sun,Can Lyu et al.
Lanmei Zhao et al.
This study presented new insights into the sustainable conversion of total petroleum hydrocarbon (TPHC) into polyhydroxyalkanoates (PHAs) using wetland microbial fuel cells (WMFCs). The main innovations included the following two points: (1...
Optimizing longifolene production in Yarrowia lipolytica via metabolic and protein engineering [0.03%]
通过代谢工程和蛋白质工程技术优化抗绵羊亚油烯烃在裂殖酵母中的生产
Yi-Tong Yao,Xiao Zhang,Chen-Yu Wang et al.
Yi-Tong Yao et al.
Longifolene (C15H24) is a tricyclic sesquiterpene widely utilized in the cosmetics and fragrances due to its versatile applications. Traditional extraction methods from plants suffer from low titer and lengthy production cycles, while chemi...
Coupling genome-wide continuous perturbation with biosensor screening reveals the potential targets in yeast isopentanol synthesis network [0.03%]
基于全基因组连续扰动与生物传感器筛选耦合发现酵母异戊醇合成网络潜在靶点
Qi Xiao,Jingjing Shi,Lixian Wang et al.
Qi Xiao et al.
The increasing consumption of fossil fuels is contributing to global resource depletion and environmental pollution. Branched-chain higher alcohols, such as isopentanol and isobutanol, have attracted significant attention as next-generation...
Yan Zheng,Xi-Chen Cui,Fei Guo et al.
Yan Zheng et al.
Polymerase cycling assembly (PCA) stands out as the predominant method in the synthesis of kilobase-length DNA fragments. The design of overlapping regions is the core factor affecting the success rate of synthesis. However, there still exi...
Proton motive force generated by microbial rhodopsin promotes extracellular electron transfer [0.03%]
微生物视紫红质产生的质子动力促进细胞外电子传递
Wenqi Ding,Tong Lin,Yun Yang et al.
Wenqi Ding et al.
The primary limitation to the practicability of electroactive microorganisms in bioelectrochemical systems lies in their low extracellular electron transfer (EET) efficiency. The proton motive force (PMF) represents the electrochemical grad...
High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast [0.03%]
大肠杆菌和酵母中葡萄糖和木糖的共利用高效产游离脂肪酸
Xin Ni,Jingjing Li,Wei Yu et al.
Xin Ni et al.
Lignocellulose bio-refinery via microbial cell factories for chemical production represents a renewable and sustainable route in response to resource starvation and environmental concerns. However, the challenges associated with the co-util...
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus [0.03%]
放线菌GvmR和GvmR2调控GVME合成的机制研究
Haoran Shi,Jiabin Wang,Shanshan Li et al.
Haoran Shi et al.
Guvermectin, a purine nucleoside natural product produced by the genus Streptomyces, has recently been registered as a new biopesticide to boost rice yield. Despite its economic and agricultural significance, the regulatory mechanisms of gu...
Genetically-modified activation strategy facilitates the discovery of sesquiterpene-derived metabolites from Penicillium brasilianum [0.03%]
遗传操控激活策略促进Penicillium brasilianum中倍半萜衍生代谢产物的发现
Wenni He,Xiaoting Rong,Hui Lv et al.
Wenni He et al.
Genome mining has revealed that Penicillium spp. possess numerous down-regulated or cryptic biosynthetic gene clusters (BGCs). This finding hinted that our investigation of fungal secondary metabolomes is limited. Herein, we report a geneti...
Environment signal dependent biocontainment systems for engineered organisms: Leveraging triggered responses and combinatorial systems [0.03%]
环境信号依赖的生物保藏系统：利用触发式响应和组合系统来约束工程微生物菌株的逃逸风险
Shreya Varma,Khushi Ash Gulati,Janani Sriramakrishnan et al.
Shreya Varma et al.
As synthetic biology advances, the necessity for robust biocontainment strategies for genetically engineered organisms (GEOs) grows increasingly critical to mitigate biosafety risks related to their potential environmental release. This pap...