Harnessing synthetic biology for tetraterpenoid astaxanthin production: Recent advances and challenges [0.03%]
合成生物学在四萜虾青素生产中的应用：近期进展与挑战
Yue Hou,Ailin Guan,Xuefen Fan et al.
Yue Hou et al.
Astaxanthin, a potent lipid-soluble ketocarotenoid, exists in various stereoisomeric, geometric isomeric, and esterified forms. Its unique molecular configuration and biological activity confer significant advantages for pharmaceutical appl...
CRISPR-based synthetic biology toolkit development in Candida viswanthii and functional analysis of the stress responsive Ena1-like protein [0.03%]
基于CRISPR的合成生物学工具包在Candida viswanathii中的开发及应激响应型ENA1类蛋白的功能分析
Xin-Yue Li,Kai Li,Feng-Li Zhang et al.
Xin-Yue Li et al.
Industrial strains from the Candida genus have been applied in production of enzymes, biochemicals, and single-cell protein. However, the synthetic biology manipulation tools for Candida species remain underdeveloped. In this study, a high-...
Putative membrane protein Cgl2168 and its variant enhance thermotolerance in Corynebacterium glutamicum [0.03%]
芽孢膜蛋白Cgl2168及其变异体可提升大谷氨酸棒杆菌耐热性
Ning Gao,Xiaomeng Ni,Jiao Liu et al.
Ning Gao et al.
Corynebacterium glutamicum ATCC 13032 is widely used as the chassis strain for industrial production of various amino acids, but its thermotolerance limits productivity under high temperatures. Comparative genomic analysis in this study ide...
Integrated transcriptomic and metabolomic analyses uncover novel genetic targets for enhanced l-tryptophan production in Corynebacterium glutamicum [0.03%]
整合转录组和代谢组分析在短小芽孢杆菌中发现新型基因以提高L-色氨酸的生产
Yufei Dong,Rongsheng Gao,Nan Qin et al.
Yufei Dong et al.
Corynebacterium glutamicum is a promising microbial chassis for the industrial production of l-tryptophan, an essential amino acid with diverse applications and high market value. In our previous work, we constructed an l-tryptophan-overpro...
An efficient CRISPR-Cas12a tool for iterative genome editing, streamlined minimization, and payload engineering of Pseudomonas phage S1 [0.03%]
一种用于铜绿假单胞菌噬菌体S1基因组编辑、精简和载体工程的高效CRISPR-Cas12a工具
Yanmei Liu,Zizhen Liang,Yanyun Jing et al.
Yanmei Liu et al.
The ability to precisely engineer phage genomes is critical for advancing phage-based biotechnology and therapeutic development. Here, we present a high-efficiency, scarless CRISPR-Cas12a genome editing method for a virulent Pseudomonas aer...
Engineering mixed sugar metabolic channels in Pseudomonas putida to produce vanillic acid [0.03%]
工程假单胞菌混合糖代谢通道生产香草酸
Peihan Wu,Haifeng Ding,Zhiqing Xu et al.
Peihan Wu et al.
Hydrolysis of lignocellulosic biomass produces the mixed sugars of glucose (60-70 %), xylose (20-30 %), and arabinose (2-20 %), etc. Using mixed sugars instead of pure glucose for microbial biosynthesis will reduce the cost of carbon source...
Structural and functional insights into the iminium ion intermediate in AsKslB-mediated Pictet-Spengler reaction [0.03%]
AsKslB介导的Pictet-Spengler反应中亚胺离子中间体的结构和功能见解
Yan-Bin Teng,Zhi Qiao,Chunya Xie et al.
Yan-Bin Teng et al.
Pictet-Spengler (PS) reactions are pivotal in the biosynthesis of bioactive alkaloids and pharmaceuticals, yet key structural details underlying their enzymatic catalysis remain insufficiently understood. We identified AsKslB from Actinosyn...
Efficient synthesis of l-DOPA in Escherichia coli via cofactor and enzyme engineering [0.03%]
辅因子和酶工程技术在大肠杆菌中高效合成左旋多巴
Lihao Deng,Jurong Ping,Zhuoyuan Liu et al.
Lihao Deng et al.
The global incidence of Parkinson's disease continues to rise. Levodopa (l-DOPA) is the core therapeutic drug, and efficient and sustainable production methods are needed. However, the complex metabolic pathways and the low catalytic effici...
Hao Fu,Zhiwei Zhou,Lan Fang et al.
Hao Fu et al.
Personalized gene circuit is a robust mode of cellular regulation that can manipulate intracellular gene expression to achieve desired functional regulation. However, the construction of logic circuits that automatically sense the character...
Enhanced microbial production of pyridoxine (Vitamin B6) in Bacillus subtilis via pathway and process optimization [0.03%]
通过途径和工艺优化增强枯草芽孢杆菌中维生素B6（吡哆醇）的微生物生产
Ai-Tong Jiang,Guang-Qing Du,Xu-Yang Huang et al.
Ai-Tong Jiang et al.
Vitamin B6 refers to a family of water-soluble B vitamin, which is essential for various physiological functions, including amino acid metabolism, neurotransmitter synthesis, and hemoglobin synthesis. The biosynthesis of pyridoxine (PN), a ...