Truncation of LPD1 promoter and adaptive evolution increase cytosolic acetyl-CoA supply in yeast [0.03%]
LPD1启动子截短及适应性进化提高酵母胞液乙酰辅酶A水平
Ling Qin,Shoujie He,Dan Yuan et al.
Ling Qin et al.
Acetyl-CoA is a central metabolic intermediate that serves as a key precursor for the biosynthesis of high-value compounds such as terpenoids. However, its compartmentalization within Saccharomyces cerevisiae limits its availability in the ...
Engineering genetic elements for microbial protein expression systems: Advances, challenges, applications, and prospects [0.03%]
用于微生物蛋白表达系统的遗传元件的工程化：进展、挑战、应用和前景
Xiaoqian Li,Cuifang Ye,Tao Liu et al.
Xiaoqian Li et al.
The rising global demand for sustainable protein sources poses critical challenges across food, pharmaceutical, and industrial biotechnology sectors. Microbial expression systems provide scalable and versatile platforms for producing recomb...
Amplicon-based prediction of secondary metabolic potential of microbiomes facilitates natural product discovery [0.03%]
基于扩增子的微生物组次级代谢潜力预测促进天然产物发现
Zhen-Yi Zhou,Qun-Jian Yin,Buddha Bahadur Basnet et al.
Zhen-Yi Zhou et al.
The rapid exploration of natural products not only expands chemical diversity but also plays a vital role in drug discovery by identifying bioactive agents (e.g., antibiotics), revealing novel biological mechanisms, and providing new therap...
Competitive binding of TeXlnR and TeAmyR regulates cellulase production in Talaromyces endophyticus [0.03%]
TEXlnR和TeAmyR的竞争结合调控内生真菌Talaromyces的纤维素酶产生
Yixin Sun,Qi Sun,Tiantian Gao et al.
Yixin Sun et al.
Efficient cellulase production is essential for its application in green bioprocessing, yet the transcriptional regulation of cellulase biosynthesis in Talaromyces sp. remains largely uncharacterized. In this study, we elucidate the regulat...
Multiple Buster transposons from hAT superfamily displaying transposition activities in mammal cells [0.03%]
来自hAT超家族的多个Buster转座子在哺乳动物细胞中表现出转座活性
Zhongxia Guan,Binqing Wang,Bo Gao et al.
Zhongxia Guan et al.
TcBuster, a member of the Buster family within the hAT superfamily, has been engineered into an effective gene transfer tool for gene therapy applications. To diversify the toolkit available for efficient gene delivery, we conducted a bioin...
A simplified synthetic microbial community enhances resistance of crucian carp (Carassius auratus) to Aeromonas hydrophila infection through host immune activation [0.03%]
通过宿主免疫激活增强麦穗鱼(Aeromonas hydrophila)对感染的抵抗力的简化合成微生物群落
Xiaozhou Qi,Yilin Zhang,Zhongchen Sun et al.
Xiaozhou Qi et al.
Bacterial diseases represent a major bottleneck in the sustainable development of aquaculture. The gut microbiota plays a vital role in host growth and health, including the enhancement of disease resistance. Although substantial progress h...
Harnessing prokaryotic amino acid transporters for metabolic engineering: mechanisms and biotechnological applications [0.03%]
工程化利用微生物氨基酸转运蛋白的机制及生物技术应用
Junyao Huang,Wenming Wu,Zhicheng Fu et al.
Junyao Huang et al.
As fundamental bioactive molecules and building blocks of cellular structures, amino acids play a crucial role in sustaining life, reflected in a global market valued at tens of billions of dollars. Recently, microbial fermentation has beco...
Engineering heterologous core promoters to optimize the SES expression system for Pichia pastoris [0.03%]
工程化异源核心启动子以优化毕赤酵母表达体系中分泌型工程化丝氨酸恩zyme的表达
Bo Sun,Yu Tan,Nitesh Kumar Mund et al.
Bo Sun et al.
Synthetic biology has revolutionized microbial production by broadening the range of substrates, products, and host organisms. Pichia pastoris, a pivotal system for industrial enzyme production and biopharmaceuticals, encounters limitations...
Developing the protein quality control system in Streptomyces for selective translation of ultra-long full-length polyketide synthase mRNAs to enhance biosynthesis efficiency [0.03%]
开发链霉菌蛋白质质量控制系统，选择性地翻译超长全长聚酮合酶mRNA以增强生物合成效率
Chanjuan Jiang,Kexin Hao,Shaoqian Wang et al.
Chanjuan Jiang et al.
Genes that encode type I polyketide synthases (PKSs), which synthesize an array of invaluable drugs, typically exceed 10 kilobases in length. Truncated messenger RNAs (mRNAs) have been demonstrated to constitute the majority of PKS mRNAs; H...
CRISPy-web 3.0: A unified platform for multi-modal guide RNA design for CRISPR and TnpB genome editing applications [0.03%]
CRISPy-web 3.0：一个用于多种导向RNA设计的统一平台，应用于CRISPR和TnpB基因组编辑中
Sihan Yang,Zupeng Cai,Natalie Chia et al.
Sihan Yang et al.
CRISPy-web is an interactive web-based platform designed for rational guide RNA design in CRISPR-Cas9-based genome editing systems. Here, we present CRISPy-web 3.0 (https://crispy.secondarymetabolites.org/), an upgraded and versatile versio...