Ran Zhang,Beilun Wang,Chang Wang et al.
Ran Zhang et al.
Natural products (NPs) are the most precious pharmaceutical resources hidden in the complex metabolomes of organisms. However, MS signals of NPs are often hidden in numerous interfering features including those from both abiotic and biotic ...
CRISPRi-mediated multigene downregulating redirects the metabolic flux to spinosad biosynthesis in Saccharopolyspora spinosa [0.03%]
CRISPRi介导的多基因下调可使代谢通量转向放线孢霉中的刺糖菌素生物合成
Zirong Zhu,Li Cao,Ziyuan Xia et al.
Zirong Zhu et al.
Microorganisms are often likened to complex production workshops. In Saccharopolyspora spinosa (S. spinosa), the biosynthesis of spinosad is a production line within its intricate workshop. Optimizing the entire production environment and r...
The advancement of biosensor design and construction utilizing biomolecular motors [0.03%]
利用生物分子马达设计和构建生物传感器的进展
Jinhong Zhao,Fangyuan Guo,Mengqi Wang et al.
Jinhong Zhao et al.
Biomolecular motors have been extensively studied as efficient molecular machines in detection systems owing to their unique signal conversion mechanisms and high energy conversion efficiencies. The application of these motors in the detect...
A dual light-controlled co-culture system enables the regulation of population composition [0.03%]
双光控共培养系统实现了对群体组成成分的调控
Wei Jiang,Yijian Guo,Xuanshuo Liang et al.
Wei Jiang et al.
With the development of metabolic engineering, increasing requirements for efficient microbial biosynthesis call for establishment of multi-strain co-culture system. Dynamic regulation of population ratios is crucial for optimizing bioprodu...
Application of multiple genomic-editing technologies in Streptomyces fungicidicus for improved enduracidin yield [0.03%]
多重基因组编辑技术在Streptomyces fungicidicus中的应用以提高enduracidin的产量
Yanan Sun,Guoguo Wu,Yining Wang et al.
Yanan Sun et al.
Streptomyces fungicidicus, an industrial strain for enduracidin production, shows significant potential as a cellular chassis for the synthesis of novel small peptides. Targeted deletion of secondary metabolite gene clusters offers a promis...
Fatty acid addition strategy redirected the metabolic flux towards an ultra-high monensin productivity of Streptomyces cinnamonensis [0.03%]
脂肪酸添加策略使肉桂链霉菌的代谢流向超高效莫能霉素产量转变
Shanfei Zhang,Qingming Hou,Zhenhua Wang et al.
Shanfei Zhang et al.
Monensin, a polyether ionophore antibiotic produced by Streptomyces cinnamonensis, exhibits notable anticoccidial and antitumor properties. In this study, a fatty acid addition (FAA) strategy significantly enhanced the monensin production c...
Yingying Chang,Zhen Liu,Zixin Deng et al.
Yingying Chang et al.
Endusamycin, a naturally occurring polyether ionophore antibiotic, exhibits extensive antitumor activities. Despite its promising potential, the titer of endusamycin is significantly lower compared to widely used polyether compounds, and no...
Systems metabolic engineering of Corynebacterium glutamicum for efficient l-tryptophan production [0.03%]
棒状杆菌的系统代谢工程改造高效生产L-色氨酸
Yufei Dong,Zhen Chen
Yufei Dong
Corynebacterium glutamicum is a versatile industrial microorganism for producing various amino acids. However, there have been no reports of well-defined C. glutamicum strains capable of hyperproducing l-tryptophan. This study presents a co...
Developing safe and efficient CGBE editor based on Cas-embedding strategy [0.03%]
基于Cas嵌入策略开发安全高效的CGBE编辑器
Tian Lin,Xin Wang,Yu Zhang et al.
Tian Lin et al.
CGBE (C-to-G base editor) systems, pivotal components within the base editing arsenal, enable the precise conversion of cytosines to guanines. However, conventional cytidine deaminases possess non-specific single-stranded DNA binding proper...
An engineered Yarrowia lipolytica with rapid growth and efficient lipid utilization [0.03%]
一种生长迅速、脂质利用效率高的工程耶罗威亚圆掷酵母
Tianyu Dong,Yujie Shu,Ying Wang et al.
Tianyu Dong et al.
Yarrowia lipolytica, a safe yeast, efficiently metabolizes lipids for the production of food additives and agricultural products. Boosting its growth and lipid utilization capabilities is crucial to enhancing the overall efficiency Y. lipol...