Optimized dual-AAV base editor delivery system with enhanced editing efficiency and virion production titer [0.03%]
一种高效病毒载体生产的优化双AAV碱基编辑系统
Wenjia Yu,Yujie Wang,Siwei Li et al.
Wenjia Yu et al.
Base editors (BEs) are a promising tool for precise base conversion in human cells and animals, while the adeno-associated virus (AAV) is the major vector for human gene therapy. However, the size of the DNA cassette required for BE express...
Enhanced chlorogenic acid production from glucose via systematic metabolic engineering of Saccharomyces cerevisiae [0.03%]
通过系统性代谢工程改造酿酒酵母细胞从葡萄糖高效生产绿原酸
Shuai Tu,Junjie Wang,Pengming Yang et al.
Shuai Tu et al.
Chlorogenic acid (CGA) is a valuable phenolic acid with various pharmaceutical functions. In our previous study, de novo synthesis of CGA in Saccharomyces cerevisiae was achieved. However, its yield required improvement before large scale p...
Peilin Shen,Yukui Zheng,CongYu Zhang et al.
Peilin Shen et al.
DNA storage, characterized by its durability, data density, and cost-effectiveness, is a promising solution for managing the increasing data volumes in healthcare. This review explores state-of-the-art DNA storage technologies, and provides...
ActinoMation: A literate programming approach for medium-throughput robotic conjugation of Streptomyces spp [0.03%]
ActinoMation：一种中通量机器人介导的链霉菌接合共培养的文献编程方法
Tenna A Møller,Thomas J Booth,Simon Shaw et al.
Tenna A Møller et al.
The genus Streptomyces are valuable producers of antibiotics and other pharmaceutically important bioactive compounds. Advances in molecular engineering tools, such as CRISPR, have provided some access to the metabolic potential of Streptom...
System-level characterization of engineered and evolved formatotrophic E. coli strains [0.03%]
工程进化型formatotrophic大肠杆菌菌株的系统水平特征分析
Suzan Yilmaz,Boas Kanis,Rensco A H Hogers et al.
Suzan Yilmaz et al.
One-carbon compounds, such as formate, are promising and sustainable feedstocks for microbial bioproduction of fuels and chemicals. Growth of Escherichia coli on formate was recently achieved by introducing the reductive glycine pathway (rG...
Efficient production of hydroxysalidroside in Escherichia coli via enhanced glycosylation and semi-rational design of UGT85A1 [0.03%]
通过增强的糖基化和UGT85A1的半理性设计在大肠杆菌中高效生产水杨苷葡萄糖酯
Xinru Wang,Lian Wang,Qihang Chen et al.
Xinru Wang et al.
Hydroxysalidroside is an important natural phenylethanoid glycoside with broad application prospects in the food and pharmaceutical fields. However, its low concentration in plants and complex extraction hinder its production. Despite being...
The biosynthesis of trillin 6'- O-glucoside: A low-abundance yet pharmacologically active polyphyllin from Paris polyphylla [0.03%]
三七素6'-O-葡萄糖苷的生物合成：来自参花粉中的一种低丰度但具有药理活性的多叶皂苷成分
Yaran Suo,Shuyu Li,Haining Lyu et al.
Yaran Suo et al.
Natural products from medicinal plants serve as an invaluable resource for drug discovery and development. However, low-abundance natural products are often understudied due to the challenges of obtaining sufficient quantities for pharmacol...
CRISPR/Cas9-based iterative multi-copy integration for improved metabolite yields in Saccharomyces cerevisiae [0.03%]
基于CRISPR/Cas9的多次迭代多拷贝整合以提高酿酒酵母中的代谢产物的产量
Ximei Chen,Chenyang Li,Xin Qiu et al.
Ximei Chen et al.
High-copy integration of key genes offers a promising strategy for efficient biosynthesis of valuable natural products in Saccharomyces cerevisiae. However, traditional multi-copy gene integration methods meet challenges including low effic...
Exploration and mutagenesis of the germacrene A synthase from Solidago canadensis to enhance germacrene A production in E. coli [0.03%]
加拿大千里光牻牛儿烯A合酶的探索与突变以提高大肠杆菌中牻牛儿烯A的产量
Jinyan Huo,Xiaohui Chu,Bo Hong et al.
Jinyan Huo et al.
β-elemene is an effective anti-cancer component which has been widely used in clinic. However, it still relies on the extraction from the Chinese medicine plant Curcuma wenyujin, which seriously limits its application. Synthetic biology of...
Sher Ali,Ashley Suris,Yun Huang et al.
Sher Ali et al.
Ion channels play instrumental roles in regulating membrane potential and cross-membrane signal transduction, thus making them attractive targets for understanding various physiological processes and associated diseases. Gaining a deeper un...