Programmable large-cargo integration: Overcoming size constraints for next-generation gene therapy [0.03%]
可编程的大载荷整合：下一代基因治疗中克服尺寸限制的方法
Lifang Yu,Mario Andrea Marchisio
Lifang Yu
The emergence of base and prime editors-genome editing tools that avoid double-strand breaks (DSBs)-has enabled precise point mutations, insertions, inversions, deletions, and substitutions, which accelerates the development of single-inter...
Synthesis of tunable copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate by engineered Halomonas bluephagenesis and their characterizations [0.03%]
工程化海洋细菌Halomonas bluephagenesis合成及表征3-羟基丁酸和3-羟基戊酸共聚物
Shaowei Li,Jinghui Wang,Yaoyao Zhang et al.
Shaowei Li et al.
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with a 0-30 mol% controllable range of 3HV ratios was produced by Halomonas bluephagenesis (H. bluephagenesis) and characterized. An endogenous plasmid containing scpA and scpB encoding me...
Engineering ω-transaminase for efficient dihydroxyacetone transamination in serinol biosynthesis starting from methanol [0.03%]
工程化ω-转氨酶高效合成1,3-丙二醇前体物质二羟基乙酮
Ya Wu,Chonghao Guo,Lizhen Deng et al.
Ya Wu et al.
Serinol (2-amino-1,3-propanediol) is an important pharmaceutical intermediate, but conventional chemical or microbial routes are hampered by high energy demand, product toxicity, or complex regulation. Here, we report a modular cell-free en...
Combining multiple metabolic strategies for efficient production of longifolene in Saccharomyces cerevisiae [0.03%]
在酿酒酵母中结合多种代谢策略高效生产长叶烯
Yachao Xin,Jingping Du,Weiqiang Zhang et al.
Yachao Xin et al.
Longifolene is a sesquiterpene commonly found in the heavy turpentine oil of pine plants, with various applications ranging from pest control and fragrance production to synthetic biofuels. While S. cerevisiae cell factories can effectively...
Defect-complementation homologous recombination: A novel strategy for precise genome engineering of virulent phages [0.03%]
缺陷互补同源重组：一种用于毒力噬菌体精确基因组工程的新策略
Hailin Zhang,Yueyue Song,Wenyue Liu et al.
Hailin Zhang et al.
Engineered bacteriophages (phages) have been developed to overcome the limitations of natural phage therapies and serve as precision-targeted agents against drug-resistant bacterial infections. However, their application has been constraine...
Synergistic engineering PETase reveals loop-region mutations for enhanced catalytic activity and thermal stability [0.03%]
工程化协同PET酶揭示了用于增强催化活性和热稳定性的环区突变
Yuxin Han,Shilong Xing,Mingzhu Ding et al.
Yuxin Han et al.
Biodegradation of Polyethylene Terephthalate (PET) offer a strategic avenue for addressing the global plastic pollution crisis. However, the degradation performance of PET hydrolases remains a technological bottleneck for the industrial rea...
High-level production of vitamin K2 in Escherichia coli via modular molecular engineering [0.03%]
模块化分子工程在大肠杆菌中高效生产维生素K2
Zelin Lu,Zhongshi Huang,Zhengyin Wu et al.
Zelin Lu et al.
Menaquinone-7 (MK-7), a key form of vitamin K2 with wide-ranging nutritional and pharmaceutical applications, has attracted increasing interest for microbial production. Here, we developed an integrated modular metabolic engineering strateg...
Combinatorial engineering of enzyme and pathway for efficient β-farnesene bioproduction in Yarrowia lipolytica [0.03%]
通过组合工程实现高效β-法尼烯生物合成路径及酵酶的构建与应用——以解脂耶氏酵母为宿主细胞体系的研究案例
Hongyang Chen,Liqiu Su,Zhen Yao et al.
Hongyang Chen et al.
β-farnesene, a natural sesquiterpene compound, has gained significant attention due to its versatile applications in agriculture, industry, biofuels, and related fields. Microbial biosynthesis offers an environmentally sustainable approach...
Engineered dual-fluorescence functional nucleic acid-based CRISPR/Cas12a biosensor for label-free ratiometric detection of site-specific DNA methylation [0.03%]
一种基于工程化双荧光功能核酸的CRISPR/Cas12a生物传感器实现无标记比值法检测特定位点DNA甲基化
Wen Tian,Songcheng Yu,Kaiyang Zhang et al.
Wen Tian et al.
The CRISPR/Cas12a system holds significant promise for biomedical applications. Nevertheless, the commonly used reporter, fluorophore-quencher-labeled substrates, is hindered by labor-intensive synthesis procedures and high costs, while als...
Multi-omics elucidation of resource allocation for enhanced ethanol production via precise glucose control in anaerobic Saccharomyces cerevisiae fermentation [0.03%]
基于多组学的资源分配解析在厌氧酿酒酵母发酵中通过精确葡萄糖控制增强乙醇生产的机制研究
Zhihao Liu,Linghong Zheng,Xiaofei Yu et al.
Zhihao Liu et al.
Ethanol, a high-demand clean energy source, is primarily produced via fed-batch fermentation in industrial settings. Although our previous study identified an optimal glucose concentration of 30 g/L for maximal ethanol yield, the mechanisms...