A systematic study of regulating inorganic polyphosphates production in Saccharomyces cerevisiae [0.03%]
系统调节酿酒酵母中无机多聚磷酸盐产生的研究
Zipeng Chen,Yanling Wu,Lingfeng Qin et al.
Zipeng Chen et al.
Inorganic polyphosphate (polyP), a linear polymer of orthophosphate residues, plays critical roles in diverse biological processes spanning blood coagulation, immunomodulation, and post-translational protein modifications in eukaryotes. Not...
Programming anti-ribozymes to sense trigger RNAs for modulating gene expression in mammalian cells [0.03%]
编程抗核糖酶以感应触发RNA在哺乳动物细胞中调控基因表达
Wenhui Zhang,Shi Zhao,Mengyuan Wang et al.
Wenhui Zhang et al.
Synthetic RNA-based switches provide distinctive merits in modulating gene expression. Simple and flexible RNA-based switches are crucial for advancing the field of gene regulation, paving the way for innovative tools that can sense and man...
Enhanced l-serine synthesis in Corynebacterium glutamicum by exporter engineering and Bayesian optimization of the medium composition [0.03%]
谷氨酸棒杆菌通过转运蛋白工程和贝叶斯优化培养基组成强化l-丝氨酸合成
Yifan Huang,Yujie Gao,Yamin Huang et al.
Yifan Huang et al.
l-serine is a versatile, high value-added amino acid, widely used in food, medicine and cosmetics. However, the low titer of l-serine has limited its industrial production. In this study, a cell factory without plasmid for efficient product...
MTD: A cloud-based omics database and interactive platform for Myceliophthora thermophila [0.03%]
基于云的组学数据库和互交平台MTD用于球毛壳菌（Myceliophthora thermophila）
Jiacheng Dong,Zhitao Mao,Haoran Li et al.
Jiacheng Dong et al.
Nowadays, biological databases are playing an increasingly critical role in biological research. Myceliophthora thermophila is an excellent thermophilic fungal chassis for industrial enzyme production and plant biomass-based chemical synthe...
Development of ultrasound-visualized tumor-targeting engineered bacteria for precise tumor therapy [0.03%]
用于精确肿瘤治疗的超声可视化的肿瘤靶向工程菌的开发
Li Qu,Zhou Chi,Zhen-Ping Zou et al.
Li Qu et al.
In situ imaging diagnosis and precise treatment of deep tumor tissues are hotspots in life sciences and medical research. In recent years, using focused ultrasound to remotely control engineered bacteria for drug release has become one of t...
Combined metabolic and enzymatic engineering for de novo biosynthesis of δ-tocotrienol in Yarrowia lipolytica [0.03%]
用于新型生物合成δ-托可汀烯的代谢和酶工程技术相结合在解脂耶氏酵母中的应用
Jinbo Xiang,Mengsu Liu,Xinglong Wang et al.
Jinbo Xiang et al.
δ-Tocotrienol, an isomer of vitamin E with anti-inflammatory, neuroprotective and anti-coronary arteriosclerosis properties, is widely used in health care, medicine and other fields. Microbial synthesis of δ-tocotrienol offers significant...
Efficient production of astaxanthin in Yarrowia lipolytica through metabolic and enzyme engineering [0.03%]
高效生产虾青素代谢和酶工程融合改造里氏裂殖酵母菌株
Chalak Najat Abdullah,Mengsu Liu,Qihang Chen et al.
Chalak Najat Abdullah et al.
Astaxanthin is a natural red carotenoid, commonly used as an additive in the pharmaceutical industry and as a nutritional supplement owing to its notable antioxidant benefits. However, a complex biosynthetic pathway poses a challenge to de ...
PEX3 gene knockout influences recombinant xylanase expression by Komagataella phaffii [0.03%]
PEX3基因敲除影响毕赤酵母表达重组木聚糖酶分泌性能
Ziwei Zhou,Wenjie Cong,Mingxuan Wang et al.
Ziwei Zhou et al.
Komagataella phaffii is a methylotrophic yeast harboring a tightly regulated alcohol oxidase promoter (P AOX1 ), which is now widely used for recombinant protein production. During P AOX1 expression phase by methanol induction, a methanol m...
Identification of two new flavone 4'- O-methyltransferases and their application in de novo biosynthesis of (2S)-hesperetin in Yarrowia lipolytica [0.03%]
两个新型黄酮4'-O-甲基转移酶的鉴定及其在假丝酵母中从头合成(2S)柚皮素的研究
Yiyun Wang,Ruiqiu Huang,Song Gao et al.
Yiyun Wang et al.
Methyltransferases are pivotal enzymes in the biosynthesis of methylated flavonoids, including (2S)-hesperetin. However, existing flavonoid 4'-O-methyltransferase (F4'OMT) enzymes typically exhibit low substrate specificity and catalytic ef...
iCASRED, a scarless DNA editing tool in E. coli for high-efficiency engineering of natural product biosynthetic gene clusters [0.03%]
用于大肠杆菌天然产物生物合成基因簇高效工程化的一种无疤DNA编辑工具iCASRED
Guosong Zheng,Jiafeng Xu,Hewei Liu et al.
Guosong Zheng et al.
Efficient gene cluster editing tools are one of the key techniques for discovering novel compounds encoded by silent natural product (NP) biosynthetic gene clusters (BGCs) in microbial genomes. Currently, in vivo BGC editing tools developed...