Regulating protein glycosylation modification enhances the synthesis of taxadiene in Saccharomyces cerevisiae [0.03%]
通过调控蛋白质糖基化修饰增强Saccharomyces cerevisiae中 Taxadiene的合成
Chenglong Zhang,Jia Wang,Longfei Zhao et al.
Chenglong Zhang et al.
Utilizing synthetic biology techniques to construct recombinant engineered cells for the synthesis of paclitaxel and its key precursors has emerged as an effective method to address the supply-demand imbalance and protect rare plant resourc...
Molecular landscape analysis of azinothricin-type natural products enables the identification of kettapeptin from Streptomyces durmitorensis [0.03%]
阿佐霉素型天然产物的分子景观分析能够从束线链霉菌中鉴定出凯特肽素
Huixue Chen,Yan Gao,Shixue Jin et al.
Huixue Chen et al.
The azinothricin family of hybrid hexadepsipeptide-polyketide natural products exhibit remarkable bioactivities, including potent antibacterial, antitumor, antimalarial and anti-inflammatory activities. However, only a few azinothricin-type...
SPIN-guided engineering of a novel (R)-amine transaminase from Fusarium albosuccineum for enantioselective synthesis of chiral piperidyl amines [0.03%]
基于SPIN的导向工程化构建来自Fusarium albosuccineum手性胺转氨酶对映体选择性合成哌啶基手性amines化合物
Ruizhou Tang,Jiahuan Li,Xiaole Yang et al.
Ruizhou Tang et al.
Aminotransferases are promising green biocatalysts for the synthesis of chiral amines, yet their limited catalytic efficiencies restrict broader industrial applications. In this study, a novel (R)-amine transaminase, FalAT, was identified f...
Biosynthesis of patchoulol via metabolic engineering the oleaginous red yeast Rhodotorula toruloides [0.03%]
代谢工程产油红酵母合成.patchoulol研究
Xiaochun Zheng,Yajun Li,Zhenhua Liu et al.
Xiaochun Zheng et al.
Patchoulol is a widely used sesquiterpenoid in perfumes, cosmetics, foods and pharmaceuticals. The plant-dependent production is suffering from limited growing area, long seasonal cycle, etc. Microbial production represents a sustainable al...
Development of a suite of activatable plant synthetic promoter systems using a bacterial LysR-type transcriptional regulator FdeR [0.03%]
基于细菌Lys型转录调控因子FdeR的植物合成启动子激活元件体系开发
Yinan Wu,Curtis Chen,Sijin Li
Yinan Wu
Advancing plant synthetic biology requires an abundant supply of orthogonal and tunable genetic parts to express multiple genes simultaneously. Current genetic parts, particularly promoters, used in plants are still limited and often suffer...
Engineering a broad-spectrum glucose oxidase via substrate channel and linker design for enhanced lignocellulose bioconversion [0.03%]
通过底物通道和连接臂设计工程化广谱型葡萄糖氧化酶用于改善木质纤维素的生物转化性能
Yong Feng,Xihua Chen,Zeyang Li et al.
Yong Feng et al.
Glucose oxidase (GOD) is a widely used enzyme in biotechnology, yet its narrow substrate specificity limits its application in complex bioconversion processes such as agricultural waste valorization. In this study, we employed synthetic bio...
Antibiotic-free high-yield (-)-α-bisabolol production in Serratia marcescens via metabolic engineering and genomic integration of mevalonate pathway genes [0.03%]
无抗生素高产(-)-α-冰片醇产酸菌的代谢工程及甲羟戊酸途径基因全基因组整合研究
Wei Li,Di Liu,Linbo Gou et al.
Wei Li et al.
(-)-α-Bisabolol, a valuable monocyclic sesquiterpene alcohol, has garnered significant attention in the pharmaceutical and cosmetic industries due to its remarkable anti-inflammatory, antibacterial, and skin-care properties. In this study,...
A growth-coupled progesterone-responsive biosensor for high-throughput microfluidic screening in Saccharomyces cerevisiae [0.03%]
一种用于酵母高通量微流体筛选的与生长偶联的孕酮响应生物传感器
Yucheng Hu,Jinde Chen,Shaofang Tian et al.
Yucheng Hu et al.
Poor aqueous solubility of steroid precursors, such as pregnenolone and progesterone, limits microbial biotransformation and high-throughput strain screening, representing a bottleneck for strain improvement and potential industrial applica...
Metabolic engineering enables Escherichia coli to grow on 1,3-propanediol [0.03%]
代谢工程使大肠杆菌能够利用1,3-丙二醇生长
Nga Yu Poon,Anthony J Sinskey,Kang Zhou
Nga Yu Poon
1,3-propanediol (1,3-PDO) is used to synthesize plastics used in many consumer products. As the demand and production of such plastics increase, a technology will be needed to utilize 1,3-PDO released from the plastics after their disposal....
Leveraging ANXA1 to enhance recombinant protein yields in CHO cells: A UPR-Mediated bioprocessing approach [0.03%]
利用ANXA1提高CHO细胞重组蛋白产量的UPR介导生物加工方法
Qi Zhao,Hui-Jie Zhang,Ming-Ming Han et al.
Qi Zhao et al.
Chinese hamster ovary (CHO) cells undergo endoplasmic reticulum stress (ERS) during intensive recombinant protein production, triggering the unfolded protein response (UPR) to balance cell survival and protein output. Nevertheless, key regu...