A combinatorial transcription factor screening platform for immune cell reprogramming [0.03%]
一种用于免疫细胞重编程的组合型转录因子筛选平台
Ilia Kurochkin,Abigail R Altman,Inês Caiado et al.
Ilia Kurochkin et al.
Direct reprogramming of immune cells holds promise for immunotherapy but is constrained by limited knowledge of transcription factor (TF) networks. Here, we developed REPROcode, a combinatorial single-cell screening platform to identify TF ...
Disobind: A sequence-based, partner-dependent contact map and interface residue predictor for intrinsically disordered regions [0.03%]
基于序列的、依赖于互作蛋白的伴侣依赖性接触图和界面残基预测工具用于内在无序区域
Kartik Majila,Varun Ullanat,Shruthi Viswanath
Kartik Majila
Intrinsically disordered proteins or regions (IDPs or IDRs) adopt diverse binding modes with different partners, ranging from coupled folding and binding to fuzzy binding and fully disordered binding. Characterizing IDR interfaces is challe...
DynamicGT: A dynamic-aware geometric transformer model to predict protein-binding interfaces in flexible and disordered regions [0.03%]
动态感知几何变换模型预测柔性无序区的蛋白质结合界面
Omid Mokhtari,Sergei Grudinin,Yasaman Karami et al.
Omid Mokhtari et al.
Protein-protein interactions are fundamental to cellular processes, yet current deep learning approaches for binding site prediction rely on static structures, limiting their accuracy for disordered or flexible regions. We introduce dynamic...
Core passive and facultative mTOR-mediated mechanisms coordinate mammalian protein synthesis and decay [0.03%]
核心被动和辅助的mTOR介导机制协调哺乳动物蛋白质合成和降解
Michael Shoujie Sun,Benjamin Martin,Joanna Dembska et al.
Michael Shoujie Sun et al.
The maintenance of cellular homeostasis requires tight regulation of proteome concentration and composition. To achieve this, protein production and elimination must be robustly coordinated. However, the mechanistic basis of this coordinati...
Engineering mammalian protein secretion: Toward the convergence of high-throughput biology and computational methods [0.03%]
工程化哺乳动物蛋白分泌：高通量生物学与计算方法的结合之路
Jacopo Gabrielli,Nathan E Lewis,Cleo Kontoravdi et al.
Jacopo Gabrielli et al.
Protein secretion in mammalian cells is the active transport of proteins from the cytoplasm to the extracellular space. It plays a fundamental role in mammalian physiology and signaling, as well as biotherapeutics production and cell and ge...
Ectopic expression to synthetic design: Deriving engineering principles of lncRNA-mediated epigenetic regulation [0.03%]
从异位表达到合成设计：衍生长非编码RNA介导的表观遗传调控工程学原理
Shalley Sharma,Seong Hu Kim,Tian Hong et al.
Shalley Sharma et al.
A key challenge in synthetic biology is achieving durable amplification of low-level inputs in gene regulation systems. Current RNA-based tools primarily operate post-transcriptionally and often yield limited, transient responses. An undere...
Integrating synthetic biology to understand and engineer the heart, lung, blood, and sleep systems [0.03%]
合成生物学在心脏、肺、血液和睡眠系统研究中的应用及展望
Elliot L Chaikof,Jichao Chen,Martha U Gillette et al.
Elliot L Chaikof et al.
Synthetic biology offers control over cellular and tissue functions. As it moves beyond microbes into humans, synthetic biology enables precise control over gene expression, cell fate, and tissue organization across heart, lung, blood, and ...
Yeganeh Dorri Nokoorani,Hourieh Movasat,Enzo Giacopino et al.
Yeganeh Dorri Nokoorani et al.
Owing to the complexity of living cells, multicellular systems exhibit heterogeneity at both the macro (different cell types) and the micro (molecular states within a cell type) levels. Traditionally, such heterogeneity has challenged the y...
What do you most hope we will achieve with mammalian synthetic biology within the next decade? [0.03%]
您希望我们在哺乳动物合成生物学方面在未来十年内取得什么最重要的成就？
Martin Fussenegger,Yvonne Y Chen,Wilson Wong et al.
Martin Fussenegger et al.
John C Snell,Brian J Nelson,Kenneth A Matreyek
John C Snell
DIAL is a novel framework for temporal control of transcript abundances in engineered cells. Targeted excision of DNA spacers in transgenic promoters permits controlled transitions of protein expression between setpoints. DIAL expands the r...