Quantifying protein unfolding kinetics with a high-throughput microfluidic platform [0.03%]
一种高通量微流控平台量化蛋白质变性动力学
Beatriz Atsavapranee,Fanny Sunden,Daniel Herschlag et al.
Beatriz Atsavapranee et al.
Even after folding, proteins sample unfolded intermediates at risk of irreversible alteration (e.g., via proteolysis, aggregation, or posttranslational modification). Thus, kinetic stability impacts protein lifetime and abundance. However, ...
Roshan Ravishankar,Dan Marston,Klaus M Hahn et al.
Roshan Ravishankar et al.
Many molecular signals are known to be organized in subcellular regions termed microdomains. One example entails Rho GTPases, which control a range of cell behaviors through their functional patterning in spatiotemporal units. While fluores...
Renyu Wang,Boya Li,Shiyue Xiong et al.
Renyu Wang et al.
Protein/RNA condensates participate in many cellular processes, but their functional roles often remain unclear. We recently found that condensates of the RNA-binding protein ATXN2 oscillate with the circadian cycle, recruit translation mac...
Foundation model reveals the shared organization of transcription and topologically associating domains [0.03%]
基础模型揭示了转录和拓扑关联结构域的共同组织规律
Huan Liang,Bonnie Berger,Rohit Singh
Huan Liang
The three-dimensional organization of chromatin into topologically associating domains (TADs) may impact gene regulation by bringing distant genes into contact. However, studies of TADs' function and their influence on transcription have be...
Evaluating SARS-CoV-2 antibody resilience via prediction and design of escape viral variants [0.03%]
通过预测和设计逃逸型病毒变异株来评估SARS-CoV-2抗体的抵抗力
Marian Huot,Pierre Rosenbaum,Cyril Planchais et al.
Marian Huot et al.
The evolutionary trajectory of SARS-CoV-2 is shaped by competing pressures for angiotensin-converting enzyme 2 (ACE2) binding, viability, and escape from neutralizing antibodies targeting its receptor-binding domain (RBD). Here, we present ...
Single-cell heterogeneity in interferon induction potential is heritable and governed by variation in cell state [0.03%]
干扰素诱导潜能的单细胞异质性可以遗传并受细胞状态变异的影响
Elizabeth A Thayer,Gabriela Shipman,Joel Rivera-Cardona et al.
Elizabeth A Thayer et al.
Type I and III interferons (IFNs) are among the first lines of defense against viral infection, yet they are generally only produced by a tiny fraction of infected cells. Here, we show that cellular heterogeneity in IFN-induction potential ...
An optogenetic smart microscopy platform reveals signaling dynamics-dependent control over collective cell migration [0.03%]
一种光遗传智能显微平台揭示信号动态依赖的集体细胞迁移调控
Harrison R Oatman,Beena C Lad,Long Nguyen et al.
Harrison R Oatman et al.
In cell biology, optical techniques can measure cells' internal states (biosensors) and stimulate cellular responses (optogenetics). Yet the design of all-optical experiments is often manual: a predetermined stimulus pattern is applied to c...
RIG-Ing the system to balance immune activation and inflammatory tissue damage in the airways [0.03%]
调节系统以在气道中平衡免疫激活和炎症组织损伤
Alec P Pankow,Margaret A Scull,Brad R Rosenberg
Alec P Pankow
Exposed to diverse pathogenic and non-pathogenic insults, the airway epithelium must balance effective host defense while minimizing unnecessary inflammation and tissue damage. In studies of influenza A virus infection including spatial tra...
Programmable regenerative tissues: Converging mammalian synthetic biology and tissue engineering [0.03%]
可编程再生组织：融合哺乳动物合成生物学和组织工程学
Alexander E Vlahos
Alexander E Vlahos
Synthetic biology can program cellular behavior but remains underused in regenerative medicine. This commentary argues that integrating synthetic biology with tissue engineering should target vascularization and immune integration through c...
Multimolecular proofreading overcomes the activity-fidelity trade-off [0.03%]
多分子校对作用克服了活性与保真度之间的权衡关系
Zhuo Mao,Yuanqi Jia,Yuxuan Yan et al.
Zhuo Mao et al.
Accurate signal processing is essential for proper cell functions and can be achieved through kinetic proofreading (KPR), where an enzyme undergoes sequential state transition and irreversible deactivation to enable high fidelity. However, ...