NOT-gated chimeric antigen receptor circuits in T and NK cells [0.03%]
T细胞和自然杀伤细胞中不含NOT gate的嵌合抗原受体电路
Seunghee Lee,Jingyao Chen,Menna Y Siddiqui et al.
Seunghee Lee et al.
Chimeric antigen receptor (CAR) T cells are powerful cancer immunotherapies, but the lack of tumor-specific single antigens for most cancers limits the therapeutic window. A NOT-gated CAR circuit, consisting of an activating CAR (aCAR) and ...
Constructing a holistic map of cell fate decision by hyper solution landscape [0.03%]
通过超解空间构建细胞命运决定的全局图谱
Xiaoyi Zhang,Zhiyuan Li,Lei Zhang
Xiaoyi Zhang
The Waddington landscape metaphor has inspired extensive quantitative studies of cell fate decisions using dynamical systems. While these approaches provide valuable insights, the intrinsic nonlinear complexity and the parameter dependence ...
Geometric quantification of cell phenotype transition manifolds with information geometry [0.03%]
基于信息几何的细胞表型过渡流形的几何量化方法研究
Miao Huang,Yuxuan Wang,Junda Ying et al.
Miao Huang et al.
Cell phenotype transition (CPT) is crucial in development and other biological processes. Advances in single-cell sequencing reveal that CPT dynamics are confined to low-dimensional manifolds, yet current methods cannot directly quantify th...
Cell Painting for cytotoxicity and mode-of-action analysis in primary human hepatocytes [0.03%]
用于人原代肝细胞的细胞毒性及作用机制分析的细胞绘制法
Jessica D Ewald,Katherine L Titterton,Alex Bäuerle et al.
Jessica D Ewald et al.
Scalable, human-relevant approaches for detecting compound modes of action are needed to improve chemical safety evaluation. Here, we apply image-based profiling (Cell Painting) alongside two cytotoxicity assays in primary human hepatocytes...
Systemic rhythmicity of host and bacterial bile acid amidates in the mouse [0.03%]
小鼠宿主和细菌胆酸酰胺的系统节律性
Erin R Reilly,Vincent Charron-Lamoureux,Helena Mannochio-Russo et al.
Erin R Reilly et al.
Bacterial bile acid amidates (BBAAs) represent an emerging class of host-microbe co-metabolites formed when gut bacteria conjugate bile acids with amino acids beyond taurine and glycine. Although these conjugates have been shown to modulate...
Context-informed subgraph foundation models enable interpretable protein-function prediction [0.03%]
基于上下文的子图基础模型能够进行可解释的蛋白质功能预测
Zhuomin Zhou,Jiahua Rao,Zhongyue Zhang et al.
Zhuomin Zhou et al.
Protein-function prediction is crucial for elucidating molecular mechanisms driving biological processes and therapeutics development. Despite numerous computational tools demonstrating promising performance, they fall short when predicting...
Antithetic integral feedback control redesigned for improved dynamics and lower noise [0.03%]
改进了动态性能和降低噪音的反向积分反馈控制
Chelsea Y Hu
Chelsea Y Hu
Integral feedback enables perfect adaptation, but antithetic implementations can be slow and noisy. Filo et al. show that sensor-based antithetic integral feedback (sAIF) control yields effective proportional-integral (PI) behavior without ...
Haplotype-resolved reconstruction and functional interrogation of cancer karyotypes [0.03%]
癌症核型的单倍体型解析重建与功能鉴定
Gregory J Brunette,Richard W Tourdot,Jinyu Wang et al.
Gregory J Brunette et al.
Complex karyotype changes are widespread in cancer genomes. A major gap in cancer genome characterization is the resolution of rearranged chromosomes with chromosome-length continuity. Here, we describe a two-tiered approach to determine th...
Single-cell epigenetic landscape, microenvironment interactions, and gene regulatory modules of non-functioning pituitary adenomas [0.03%]
无功能垂体腺瘤的单细胞表观遗传景观、微环境相互作用和基因调控模块
Zidong Zhang,Wan Sze Cheng,Keiko Taniguchi-Ponciano et al.
Zidong Zhang et al.
The epigenetic landscape and tumor microenvironment (TME) interactions of non-functioning pituitary adenomas (NFPAs), benign tumors with high morbidity and recurrence rates, are not well characterized. We completed single-nucleus (sn) multi...
Deep coverage and extended sequence reads obtained with a single archaeal protease expedite de novo protein sequencing by mass spectrometry [0.03%]
一种古细菌蛋白酶获得的深度覆盖和延长序列读段可加速从头蛋白质质谱分析流程
Laura Pérez Pañeda,Tereza Kadavá,Tatiana M Shamorkina et al.
Laura Pérez Pañeda et al.
The ability to sequence proteins without reliance on a genomic template defines a critical frontier in proteomics. This approach, known as de novo protein sequencing, is essential for applications in antibody sequencing, microbiome proteomi...