Aptamers as innovative tools for malaria diagnosis and treatment: advances and future perspectives [0.03%]
核酸适配体在疟疾诊治中的创新应用及未来前景展望
Wendy Yulieth Royero-Bermeo,Miryan Margot Sánchez-Jiménez,Juan David Ospina-Villa
Wendy Yulieth Royero-Bermeo
Malaria, caused by Plasmodium spp. parasites (P. vivax, P. falciparum, P. ovale, P. malariae, and P. knowlesi), remains a significant global health challenge, with 263 million cases and 567 000 deaths reported in 2023. Diagnosis in endemic ...
A generalized protocol for the induction of M2-like macrophages from mouse and rat bone marrow mononuclear cells [0.03%]
从小鼠和大鼠骨髓单个核细胞中诱导M2样巨噬细胞的通用方案
Ulugbek R Yakhshimurodov,Kizuku Yamashita,Kenji Miki et al.
Ulugbek R Yakhshimurodov et al.
Regardless of origin and localization, macrophages are the major immune cells that maintain homeostasis in both healthy and diseased states. However, there is no consensus on the phenotypes, functions and fates of macrophages. Existing stud...
Valentina Tirelli,Felicia Grasso,Valeria Barreca et al.
Valentina Tirelli et al.
In recent years, there has been a notable increasing interest surrounding the identification and quantification of nano-sized particles, including extracellular vesicles (EVs) and viruses. The challenge posed by the nano-sized dimension of ...
Mass photometry as a fast, facile characterization tool for direct measurement of mRNA length [0.03%]
用于直接测量mRNA长度的快速简便表征工具——质量光度法
Andrew Schmudlach,Saralynn Spear,Yimin Hua et al.
Andrew Schmudlach et al.
Oligonucleotide integrity is a critical quality attribute for many new therapeutic modalities. Current assays often measure attributes such as length using capillary electrophoresis or liquid chromatography. The length is then corroborated ...
A protocol for karyotyping and genetic editing of induced pluripotent stem cells with homology-directed repair mediated CRISPR/Cas9 [0.03%]
基于同源定向修复介导的CRISPR/Cas9诱导多能干细胞的核型分析和基因编辑方案
Silvana Lobo,Rita Barbosa-Matos,Sofia Dória et al.
Silvana Lobo et al.
CRISPR/Cas9-mediated homology-directed repair (HDR) allows precise gene editing, but its efficiency remains low for certain cell types, such as human induced pluripotent stem cells (hiPSCs). In this study, we aimed to introduce the CTNNA1: ...
Ethan Thomas Johnson,Jathin Koushal Bande,Johnson Thomas
Ethan Thomas Johnson
Subjective variability in human interpretation of diagnostic imaging presents significant clinical limitations, potentially resulting in diagnostic errors and increased healthcare costs. While artificial intelligence (AI) algorithms offer p...
Combined fluorescent in situ hybridization and F- ara-EdU staining on whole mount Hymenolepis diminuta [0.03%]
整体mount Hymenolepis diminuta上结合荧光原位杂交和F-ara-EdU染色
Mohamed Ishan,Isabell R Skipper,Tania Rozario
Mohamed Ishan
Hymenolepis diminuta is a parasitic tapeworm that utilizes rats as hosts and offers advantages over human parasitic tapeworms and free-living flatworms as a model system to study the biology and pathology of helminth infections. H. diminuta...
Optimizing drug sensitivity assays in patient-derived tumor organoids: a comparison of IC50 estimation methods and experimental parameters [0.03%]
优化患者衍生的肿瘤类器官中的药物敏感性测定：IC50估计方法和实验参数的比较
Yidan Chen,Jian Zhang,Bin Zhang et al.
Yidan Chen et al.
Patient-derived tumor organoids (PDOs) hold immense potential for personalized drug sensitivity testing, but accurate efficacy determination is crucial for clinical translation. This study investigated factors influencing the accuracy and r...
Protocol for isolation and expansion of natural killer cells from human peripheral blood scalable for clinical applications [0.03%]
从人体外周血中分离和扩增自然杀伤细胞的临床应用可扩展协议
Soumyadipta Kundu,Leonie Durkan,Michael O&#x;Dwyer et al.
Soumyadipta Kundu et al.
Natural killer (NK) cells have emerged as promising candidates for novel immunotherapy strategies against various malignancies. Their unique ability to recognize and eliminate tumour cells without prior sensitization, coupled with the secre...
Sammy Pontrelli,Uwe Sauer
Sammy Pontrelli
Polysaccharide quantification plays a vital role in understanding ecological and nutritional processes in microbes, plants, and animals. Traditional methods typically hydrolyze these large molecules into monomers using chemical methods, but...