Homology-based enzymatic DNA fragment assembly-based illumina sequencing library preparation [0.03%]
基于同源性的酶促DNA片段组装及illumina测序文库构建方法
Hiroshi Shinozuka,Shimna Sudheesh,Maiko Shinozuka et al.
Hiroshi Shinozuka et al.
The current Illumina HiSeq and MiSeq platforms can generate paired-end reads of up to 2 x 250 bp and 2 x 300 bp in length, respectively. These read lengths may be substantially longer than genomic regions of interest when a DNA sequencing l...
Use of green fluorescent protein to monitor fungal growth in biomass hydrolysate [0.03%]
利用绿色荧光蛋白监测生物质水解物中真菌的生长
Nancy N Nichols,Joshua C Quarterman,Sarah E Frazer
Nancy N Nichols
A reporter gene encoding green fluorescent protein (GFP) was introduced into the ascomycete Coniochaeta ligniaria NRRL30616, and fluorescence of cultures was monitored as a measure of cell growth. Fluorescence in the GFP-expressing strain w...
Covalent modification of primers improves PCR amplification specificity and yield [0.03%]
共价修饰的引物可以提高PCR扩增的特异性和产量
Nancy J Schoenbrunner,Amar P Gupta,Karen K Y Young et al.
Nancy J Schoenbrunner et al.
We report a method for covalent modification of primers that enhances the specificity of PCR and increases the yield of specific amplification products at the end of PCR. The introduction of thermally stable covalent modifications, such as ...
Peng Zhang,Florian D Hastert,Anne K Ludwig et al.
Peng Zhang et al.
DNA base modifications and mutations are observed in all genomes throughout the kingdoms of life. Proteins involved in their establishment and removal were shown to use a base flipping mechanism to access their substrates. To better underst...
A UV cross-linking method combined with infrared imaging to analyse RNA-protein interactions [0.03%]
一种结合红外成像的UV交联方法分析RNA-蛋白质相互作用
Tony Malmqvist,Carl Spickett,Jean-Marc Gallo et al.
Tony Malmqvist et al.
Photo cross-linking of proteins with short RNA oligomers is a classical method to study RNA-protein interactions that are implicated in many aspects of RNA metabolism and function. Most commonly, this involves the use of [γ-32P]-labeled RN...
Christian Korfhage,Evelyn Fricke,Andreas Meier et al.
Christian Korfhage et al.
Generation of monoclonal DNA clusters on a surface is a useful method for digital nucleic acid detection applications (e.g. microarray or next-generation sequencing). To obtain sufficient copies per cluster for digital detection, the single...
PrimerPooler: automated primer pooling to prepare library for targeted sequencing [0.03%]
PrimerPooler：自动化引物池准备目标测序文库
Silas S Brown,Yun-Wen Chen,Ming Wang et al.
Silas S Brown et al.
Targeted next-generation sequencing based on PCR amplification involves pooling of hundreds to thousands of primers, for preamplification and subsequent parallel single/multiplex PCR. It is often necessary to allocate the set of primers int...
An improved method for detecting telomere size differences in T-lymphocyte interphases from older people with Down syndrome with and without mild cognitive impairment [0.03%]
一种改进的方法：检测唐氏综合症老年人（伴有或不伴有轻度认知障碍）T淋巴细胞间期端粒大小差异
E C Jenkins,L Ye,E Marchi et al.
E C Jenkins et al.
Telomere size (quantified by fluorescence intensity and physical lengths) in short-term T-lymphocyte cultures from adults with Down syndrome (DS) with and without mild cognitive impairment (MCI-DS) or dementia was compared. For these studie...
CD-tagging-MS2: detecting allelic expression of endogenous mRNAs and their protein products in single cells [0.03%]
基于单细胞的内源性mRNA及其蛋白质表达的等位基因检测（CD标记质谱法）
Jonathan Sheinberger,Hodaya Hochberg,Erez Lavi et al.
Jonathan Sheinberger et al.
Discriminating between the mRNA and protein outputs of each of the alleles of an endogenous gene in intact cells, is a difficult task. To examine endogenous transcripts originating from a specific allele, we applied Central Dogma tagging (C...
Andjin Siegenthaler,Debapriya Mondal,Chiara Benvenuto
Andjin Siegenthaler
The study of animal colouration addresses fundamental and applied aspects relevant to a wide range of fields, including behavioural ecology, environmental adaptation and visual ecology. Although a variety of methods are available to measure...