RNA profile diversity across arthropoda: guidelines, methodological artifacts, and expected outcomes [0.03%]
节肢动物的 RNA 丰度谱系多样性：规范、方法学假象和预期结果
Danielle M DeLeo,Jorge L Pérez-Moreno,Hernán Vázquez-Miranda et al.
Danielle M DeLeo et al.
High-quality RNA is an important precursor for high-throughput RNA sequencing (RNAseq) and subsequent analyses. However, the primary metric used to assess RNA quality, the RNA Integrity Number (RIN), was developed based on model bacterial a...
Quantitative solid-phase assay to measure deoxynucleoside triphosphate pools [0.03%]
定量测定脱氧核糖核苷三磷酸池的固相测定法
Juan Cruz Landoni,Liya Wang,Anu Suomalainen
Juan Cruz Landoni
deoxynucleoside triphosphate (dNTPs) are the reduced nucleotides used as the building blocks and energy source for deoxyribonucleic acid (DNA) replication and maintenance in all living systems. They are present in highly regulated amounts a...
A cost-effective technique for generating preservable biomass smoke extract and measuring its effect on cell receptor expression in human bronchial epithelial cells [0.03%]
一种用于生成可保存的生物质烟提取物并测量其对人支气管上皮细胞受体表达影响的有效技术
K C Rajendra,Graeme R Zosky,Shakti D Shukla et al.
K C Rajendra et al.
Nearly half of the world's population uses biomass fuel for the purposes of cooking and heating. Smoke derived from biomass increases the risk of the development of lung diseases, including pneumonia, chronic obstructive pulmonary disease, ...
Utilization of dried and long-term stored polyacrylamide gels for the advanced proteomic profiling of mitochondrial contact sites from rat liver [0.03%]
用于来自大鼠肝脏线粒体接触位点的高级蛋白质组学分析的干燥和长期储存的聚丙烯酰胺凝胶的应用研究
Sandra Murphy,Michael Henry,Paula Meleady et al.
Sandra Murphy et al.
Following subcellular fractionation, the complexity of proteins derived from a particular cellular compartment is often evaluated by gel electrophoretic analysis. For the proteomic cataloguing of these distinct protein populations and their...
Absolute quantitation of poly(R)-3-hydroxybutyric acid using spectrofluorometry in recombinant Escherichia coli [0.03%]
采用分光荧光法定量重组大肠杆菌中聚(3-羟基丁酸)的绝对量
Mayooreshwar P Rajankar,Sapna Ravindranathan,P R Rajamohanan et al.
Mayooreshwar P Rajankar et al.
Poly(R)-3-hydroxybutyric acid (PHB) is a biodegradable natural polymer produced by microorganisms and plants under nitrogen deprivation and physiological stress. Metabolic engineering and synthetic biology approaches are underway to develop...
A fast, sensitive and cost-effective method for nucleic acid detection using non-radioactive probes [0.03%]
一种利用非放射性探针检测核酸的快速、灵敏且低成本的方法
David Viterbo,Astrid Marchal,Valentine Mosbach et al.
David Viterbo et al.
Nucleic acid detection and quantification using a labeled DNA probe is a very common molecular biology procedure. Here, we describe a new method, based on commonly used laboratory solutions, for nucleic acid hybridization and detection with...
An array-based melt curve analysis method for the identification and classification of closely related pathogen strains [0.03%]
一种基于数组的熔解曲线分析方法，用于鉴定和分类密切相关的病原菌株
Arjang Hassibi,Jessica Ebert,Sara Bolouki et al.
Arjang Hassibi et al.
PCR-based techniques are widely used to identify disease causing bacterial and viral pathogens, especially in point-of-care or near-patient clinical settings that require rapid results and sample-to-answer workflows. However, such technique...
Single-round isolation of diverse RNA aptamers from a random sequence pool [0.03%]
从随机序列库中一次性分离多种多样的RNA适配体
Masahiko Imashimizu,Masaki Takahashi,Ryo Amano et al.
Masahiko Imashimizu et al.
Aptamers are oligonucleotide ligands with specific binding affinity to target molecules. Generally, RNA aptamers are selected from an RNA pool with random sequences, using the technique termed SELEX, in which the target-binding RNA molecule...
A highly sensitive trap vector system for isolating reporter cells and identification of responsive genes [0.03%]
一种高灵敏度的报告基因细胞捕获载体系统及其应用
Kosuke Ishikawa,Yuta Kobayashi,Yutaro Wakabayashi et al.
Kosuke Ishikawa et al.
We devised a versatile vector system for efficient isolation of reporter cells responding to a certain condition of interest. This system combines nontoxic GAL4-UAS and piggyBac transposon systems, allowing application to mammalian cells an...
Tobias Anton,Elisabeth Karg,Sebastian Bultmann
Tobias Anton
Since the discovery of the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system (Cas) as a tool for gene editing a plethora of locus-specific as well as genome-wide approaches have been developed that ...