Immunofluorescence and image analysis pipeline for Drosophila motor neurons [0.03%]
适用于果蝇运动神经元的免疫荧光及图像分析流程
Jeremy R Brown,Chanpasith Phongthachit,Mikolaj J Sulkowski
Jeremy R Brown
The neuromuscular junction (NMJ) of larval Drosophila is widely used as a genetic model for basic neuroscience research. The presynaptic side of the NMJ is formed by axon terminals of motor neurons, the soma of which reside in the ventral g...
Live imaging of intracellular pH in planarians using the ratiometric fluorescent dye SNARF-5F-AM [0.03%]
用于.planaria.的比率型荧光染料SNARF-5F-AM的细胞内pH值实时成像研究
Wendy Scott Beane,Dany Spencer Adams,Junji Morokuma et al.
Wendy Scott Beane et al.
Physiological parameters such as resting potential and pH are increasingly recognized as important regulators of cell activity and tissue-level events in regeneration, development, and cancer. The availability of fluorescent reporter dyes h...
Construction of an overexpression library for Mycobacterium tuberculosis [0.03%]
构建结核杆菌基因过量表达的噬菌体展示文库
Eduard Melief,Rachel Kokoczka,Megan Files et al.
Eduard Melief et al.
There is a pressing need to develop novel anti-tubercular drugs. High-throughput phenotypic screening yields chemical series that inhibit bacterial growth. Target identification for such series is challenging, but necessary for optimization...
A method for the efficient and selective identification of 5-hydroxymethyluracil in genomic DNA [0.03%]
一种高效特异鉴定基因组DNA中5-羟甲基尿嘧啶的方法
Whitney Bullard,Rudo Kieft,Robert Sabatini
Whitney Bullard
Recently, 5-hydroxymethyluracil (5hmU) was identified in mammalian genomic DNA as an oxidative product of thymine by the ten-eleven translocation (TET) proteins. While the biological role of this modification remains unclear, identifying it...
Assessment of configurations and chemistries of bridged nucleic acids-containing oligomers as external guide sequences: a methodology for inhibition of expression of antibiotic resistance genes [0.03%]
含桥连核苷酸的寡核苷酸导向序列抑制抗生素抗性基因表达的方法学及构型评估
Alexis Jackson,Saumya Jani,Carol Davies Sala et al.
Alexis Jackson et al.
EGSs (external guide sequences) are short antisense oligoribonucleotides that elicit RNase P-mediated cleavage of a target mRNA, which results in inhibition of gene expression. EGS technology is used to inhibit expression of a wide variety ...
Association of cognitive deficits with sociodemographic characteristics among adults with post-COVID conditions: Findings from the United States household pulse survey [0.03%]
美国家庭脉搏调查：新冠肺炎后综合症成人认知缺陷与社会人口统计学特征相关的研究结果
People infected with coronavirus disease-19 (COVID-19) may continue to experience symptoms for several weeks or even months after acute infection, a condition known as long COVID. Cognitive problems such as memory loss are among the most co...
Integrating genomics with diverse data modalities has the potential to revolutionize personalized medicine. However, this integration poses significant challenges due to the fundamental differences in data types and structures. The vast siz...