An improved method for detecting telomere size differences in T-lymphocyte interphases from older people with Down syndrome with and without mild cognitive impairment [0.03%]
一种改进的方法：检测唐氏综合症老年人（伴有或不伴有轻度认知障碍）T淋巴细胞间期端粒大小差异
E C Jenkins,L Ye,E Marchi et al.
E C Jenkins et al.
Telomere size (quantified by fluorescence intensity and physical lengths) in short-term T-lymphocyte cultures from adults with Down syndrome (DS) with and without mild cognitive impairment (MCI-DS) or dementia was compared. For these studie...
CD-tagging-MS2: detecting allelic expression of endogenous mRNAs and their protein products in single cells [0.03%]
基于单细胞的内源性mRNA及其蛋白质表达的等位基因检测（CD标记质谱法）
Jonathan Sheinberger,Hodaya Hochberg,Erez Lavi et al.
Jonathan Sheinberger et al.
Discriminating between the mRNA and protein outputs of each of the alleles of an endogenous gene in intact cells, is a difficult task. To examine endogenous transcripts originating from a specific allele, we applied Central Dogma tagging (C...
Andjin Siegenthaler,Debapriya Mondal,Chiara Benvenuto
Andjin Siegenthaler
The study of animal colouration addresses fundamental and applied aspects relevant to a wide range of fields, including behavioural ecology, environmental adaptation and visual ecology. Although a variety of methods are available to measure...
Assessment of phagocytic activity in live macrophages-tumor cells co-cultures by Confocal and Nomarski Microscopy [0.03%]
共聚焦和诺玛斯基显微镜活细胞肿瘤-巨噬细胞共培养的吞噬活性分析
Dalia Martinez-Marin,Courtney Jarvis,Thomas Nelius et al.
Dalia Martinez-Marin et al.
Macrophages have been recognized as the main inflammatory component of the tumor microenvironment. Although often considered as beneficial for tumor growth and disease progression, tumor-associated macrophages have also been shown to be det...
qPCR-based characterization of DNA fragmentation efficiency of Tn5 transposomes [0.03%]
基于qPCR的Tn5转位酶复合物DNA断裂效率表征
Vera Rykalina,Alexey Shadrin,Hans Lehrach et al.
Vera Rykalina et al.
Here, we describe an electrophoresis free assay for characterizing Tn5 transposomes fragmentation efficiency in a tagmentation reaction, in which double-stranded DNA is fragmented and tagged with adapter sequences. The assay uses plasmid DN...
EpiHRMAssay, in tube and in silico combined approach for the scanning and epityping of heterogeneous DNA methylation [0.03%]
基于试管和计算的联合表观遗传分析方法-用于异质性DNA甲基化扫描与表位分型
Marco Cirilli,Ines Delfino,Emilia Caboni et al.
Marco Cirilli et al.
Reliable and cost-effective assays with adequate sensitivity are required to detect the DNA methylation profile in plants for scientific and industrial purposes. The proposed novel assay, named EpiHRMAssay, allows to quantify the overall me...
Identification of stable reference genes for lipopolysaccharide-stimulated macrophage gene expression studies [0.03%]
识别lipopolysaccharide刺激的巨噬细胞基因表达研究中的稳定参考基因
Roshini Kalagara,Weimin Gao,Honor L Glenn et al.
Roshini Kalagara et al.
Gene expression studies which utilize lipopolysaccharide (LPS)-stimulated macrophages to model immune signaling are widely used for elucidating the mechanisms of inflammation-related disease. When expression levels of target genes are quant...
Assessment of six commercial plasma small RNA isolation kits using qRT-PCR and electrophoretic separation: higher recovery of microRNA following ultracentrifugation [0.03%]
六种商业血浆小RNA分离试剂盒的评估：qRT-PCR和电泳分析结合微离心技术提高循环microRNA回收率
Ari Meerson,Thorkil Ploug
Ari Meerson
Growing interest in blood-borne microRNAs (miRNAs) as biomarkers has led to the introduction of a number of commercial kits for isolating small RNAs from plasma/serum. We sought to compare the efficacy of six such kits in isolating miRNAs f...
Reverse-transcription quantitative PCR directly from cells without RNA extraction and without isothermal reverse-transcription: a 'zero-step' RT-qPCR protocol [0.03%]
不经RNA提取和等温反转录直接从细胞进行逆转录定量 PCR：“零步骤”RT-qPCR 协议
Petra Chovancova,Verena Merk,Andreas Marx et al.
Petra Chovancova et al.
We describe an ultra-rapid and sensitive method to quantify gene expression levels in cultured cells. The procedure is based on reverse-transcription quantitative PCR (RT-qPCR) directly from cells, without RNA extraction and without an isot...
Single-cell transcriptomics from human pancreatic islets: sample preparation matters [0.03%]
人类胰岛单细胞转录组测序：样本处理至关重要
Lori L Bonnycastle,Derek E Gildea,Tingfen Yan et al.
Lori L Bonnycastle et al.
Single-cell RNA sequencing (scRNA-seq) of human primary tissues is a rapidly emerging tool for investigating human health and disease at the molecular level. However, optimal processing of solid tissues presents a number of technical and lo...