Joan P Breyer,Jeffrey R Smith
Joan P Breyer
Genome-wide association studies bring into focus specific genetic variants of particular interest for which validation is often sought in large numbers of study subjects. Practical alternative methods are limiting for the application of gen...
Leveraging modern DNA assembly techniques for rapid, markerless genome modification [0.03%]
利用现代DNA组装技术进行快速无标记基因组编辑
Ilya B Tikh,James C Samuelson
Ilya B Tikh
The ability to alter the genomic material of a prokaryotic cell is necessary for experiments designed to define the biology of the organism. In addition, the production of biomolecules may be significantly improved by application of enginee...
Variations in 5-methylcytosine and 5-hydroxymethylcytosine among human brain, blood, and saliva using oxBS and the Infinium MethylationEPIC array [0.03%]
基于OxBS和MethylationEPIC芯片的脑、血和唾液样本中的5-甲基胞嘧啶与5-羟甲基胞嘧啶异变分析
Jeffrey A Gross,François Lefebvre,Pierre-Eric Lutz et al.
Jeffrey A Gross et al.
Investigating 5-methylcytosine (5mC) has led to many hypotheses regarding molecular mechanism underlying human diseases and disorders. Many of these studies, however, utilize bisulfite conversion alone, which cannot distinguish 5mC from its...
David G H Andrews
David G H Andrews
Many studies have been made on nematodes, especially Caenorhabditis Elegans, which are used as a model organism. In many studies, the size of the nematode is important. This article describes a method of measuring the length, volume and sur...
Mauno Vihinen
Mauno Vihinen
Bioinformatics methods are increasingly needed and used to analyze and interpret extensive datasets many of which are produced by diverse high-throughput technologies. Unfortunately, it is quite common that published articles do not contain...
Identification of Penicillium species by MALDI-TOF MS analysis of spores collected by dielectrophoresis [0.03%]
基于介电电泳收集孢子的基质辅助激光解析电离飞行时间质谱鉴定曲霉物种
Michael A Reeve,Denise Bachmann,Thelma S Caine
Michael A Reeve
In matrix-assisted laser-desorption and ionization mass spectrometry, spectral differences are frequently observed using different growth media on agar plates and/or different growth times in culture, which add undesirable analytical varian...
Beyond classic editing: innovative CRISPR approaches for functional studies of long non-coding RNA [0.03%]
超越经典编辑：用于长链非编码RNA功能研究的创新CRISPR方法
Dahlia A Awwad
Dahlia A Awwad
Long non-coding RNAs (lncRNAs) makeup a considerable part of the non-coding human genome and had been well-established as crucial players in an array of biological processes. In spite of their abundance and versatile roles, their functional...
A comprehensive comparison and overview of R packages for calculating sample entropy [0.03%]
计算样本熵的R包的全面比较与概述
Chang Chen,Shixue Sun,Zhixin Cao et al.
Chang Chen et al.
Sample entropy is a powerful tool for analyzing the complexity and irregularity of physiology signals which may be associated with human health. Nevertheless, the sophistication of its calculation hinders its universal application. As of to...
Sajjad Janfaza,Babak Khorsand,Maryam Nikkhah et al.
Sajjad Janfaza et al.
Volatile organic compounds (VOCs), produced and emitted through the metabolism of cancer cells or the body's immune system, are considered novel cancer biomarkers for diagnostic purposes. Of late, a large number of work has been done to fin...
Improving the efficiency of gene insertion in a human artificial chromosome vector and its transfer in human-induced pluripotent stem cells [0.03%]
一种人类人工染色体载体的基因导入效率的提升及其在人诱导多能干细胞中的转移能力
Yoshinori Hasegawa,Masashi Ikeno,Nobutaka Suzuki et al.
Yoshinori Hasegawa et al.
A human artificial chromosome (HAC) vector has potential to overcome the problems of stable gene expression associated with plasmid, transposon, and virus-based vectors, such as insertional mutagenesis, position effect, uncontrollable copy ...