Christoph S Börlin,David Bergenholm,Petter Holland et al.
Christoph S Börlin et al.
The decrease of sequencing cost in the recent years has made genome-wide studies of transcription factor (TF) binding through chromatin immunoprecipitation methods like ChIP-seq and chromatin immunoprecipitation with lambda exonuclease (ChI...
Target enrichment from a DNA mixture by oligoribonucleotide interference-PCR (ORNi-PCR) [0.03%]
用于DNA混合样本分型的寡核糖核苷酸干扰 PCR(ORNi-PCR)技术
Toshitsugu Fujita,Daisuke Motooka,Hodaka Fujii
Toshitsugu Fujita
Oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) is a method that suppresses PCR amplification of target DNA in an ORN-specific manner. In this study, we examined whether ORNi-PCR can be used to enrich desirable DNA sequences from a DN...
Toshitsugu Fujita,Hodaka Fujii
Toshitsugu Fujita
In 2013, we developed a new method of engineered DNA-binding molecule-mediated chromatin immunoprecipitation that incorporates the clustered regularly interspaced short palindromic repeats (CRISPR) system to purify specific DNA species. Thi...
MALDI-TOF MS-based analysis of dried seed proteins immobilized on filter paper [0.03%]
基于MALDI-TOF MS的干燥种子蛋白滤纸固定化分析
Michael A Reeve,Kathryn M Pollard
Michael A Reeve
Matrix-assisted laser-desorption and ionization time-of-flight mass spectroscopy (MALDI-TOF MS) is commonly used for the characterization of protein-containing biological samples. For this, we have previously developed sample-preparation me...
Plasma or serum? A qualitative study on rodents and humans using high-throughput microRNA sequencing for circulating biomarkers [0.03%]
血浆还是血清？一种用于循环生物标志物的高通量微小核糖核酸测序的老鼠和人的定性研究
Thibault Dufourd,Noémie Robil,David Mallet et al.
Thibault Dufourd et al.
microRNAs are small non-coding RNAs gaining interest for their potential roles as reliable biomarkers for the diagnosis and therapeutics of numerous pathologies, ranging from cancer to neurodegenerative or psychiatric disorders. Indeed, mic...
Using SimulATe to model the effects of antibiotic selective pressure on the dynamics of pathogenic bacterial populations [0.03%]
使用SimulATe模拟抗生素选择压力对致病细菌群体动态的影响
Pedro H C David,Xana Sá-Pinto,Teresa Nogueira
Pedro H C David
Antibiotics are notable weapons in fighting bacteria. Nowadays, however, the effectiveness of antibiotics is severely hindered by the increasing levels of antibiotic resistances in pathogenic bacterial populations, which can persist due to ...
A method for filamentous fungal growth and sample preparation aimed at more consistent MALDI-TOF MS spectra despite variations in growth rates and/or incubation times [0.03%]
一种丝状真菌生长和样品制备的方法，以实现更一致的MALDI-TOF MS光谱，即使生长速率和/或孵育时间有所不同也适用。
Michael A Reeve,Denise Bachmann
Michael A Reeve
Matrix-assisted laser-desorption and ionization time-of-flight mass spectrometry can be used for the characterization and identification of filamentous fungi, for which it is desirable to have a means of growth in which the resulting spectr...
Genetic transformation of Coniochaeta sp. 2T2.1, key fungal member of a lignocellulose-degrading microbial consortium [0.03%]
丛枝菌根真菌共生网络中的植物微生物组互作机制研究——以Coniochaeta sp. 2T2为例
Nancy N Nichols,Ronald E Hector,Sarah E Frazer
Nancy N Nichols
Coniochaeta sp. strain 2T2.1 is a key member of a microbial consortium that degrades lignocellulosic biomass. Due to its ecological niche and ability to also grow in pure culture on wheat straw, protocols for transformation and antibiotic s...
Generation of 34S-substituted protein-bound [4Fe-4S] clusters using 34S-L-cysteine [0.03%]
使用~(34)S-L-半胱氨酸生成结合于蛋白的~(34)S取代的[4Fe-4S]簇离子
Jason C Crack,Melissa Y Y Stewart,Nick E Le Brun
Jason C Crack
The ability to specifically label the sulphide ions of protein-bound iron-sulphur (FeS) clusters with 34S isotope greatly facilitates structure-function studies. In particular, it provides insight when using either spectroscopic techniques ...
Enzyme-linked oligonucleotide hybridization assay for direct oligo measurement in blood [0.03%]
基于酶的寡核苷酸杂交检测技术用于直接测量血液中的寡核苷酸
Mary Y Lorenson,Kuan-Hui Ethan Chen,Ameae M Walker
Mary Y Lorenson
Small oligonucleotides (oligos) are increasingly being utilized as diagnostics or treatments for disease. An impediment to broader use is the ability to readily measure oligos in biological fluids. Here, we describe a very straightforward a...