Optimization of Chelex 100 resin-based extraction of genomic DNA from dried blood spots [0.03%]
优化Chelex 100树脂提取干血斑基因组DNA方法
Neta Simon,Jaclyn Shallat,Corey Williams Wietzikoski et al.
Neta Simon et al.
Dried blood spots (DBS) are widely utilized as part of universal newborn screening and as a means of transporting samples from field sites. We use DBS from African field sites to assess for rare maternal-fetal cell exchange during pregnancy...
Fine-mapping within eQTL credible intervals by expression CROP-seq [0.03%]
基于表达的CROP-seq在eQTL可信区间内的精细定位
Yidan Pan,Ruoyu Tian,Ciaran Lee et al.
Yidan Pan et al.
The majority of genome-wide association study (GWAS)-identified SNPs are located in noncoding regions of genes and are likely to influence disease risk and phenotypes by affecting gene expression. Since credible intervals responsible for ge...
Rapid, modular, and cost-effective generation of donor DNA constructs for CRISPR-based gene knock-in [0.03%]
快速、模块化且低成本的CRISPR基因定点整合供体质粒载体的构建方法
Yi-Jiun Chen,Ya-Yun Cheng,Weikang Wang et al.
Yi-Jiun Chen et al.
Clustered regularly interspaced short palindromic repeats (CRISPR)-based gene editing techniques find applications in many fields, such as molecular biology, cancer biology, and disease modeling. In contrast to the knock-out procedure, a ke...
Discrimination between Asian populations of the parasitoid wasp Ganaspis cf. brasiliensis using a simple MALDI-TOF MS-based method for use with insects [0.03%]
一种基于MALDI-TOF MS的简便方法用于区分寄生于亚洲地区的近缘寄生膜翅目昆虫Ganaspis cf. brasiliensis种群
Michael A Reeve,M Lukas Seehausen
Michael A Reeve
The fruit fly Drosophila suzukii has recently become an invasive pest insect of significant economic impact in Europe and the USA. In contrast to other Drosophila species, D. suzukii is able to infest intact fruit by means of a saw-like ovi...
Anna Chiara Pirona,Risky Oktriani,Michael Boettcher et al.
Anna Chiara Pirona et al.
The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency of cell transduction. Low efficiencie...
Eliminating primer dimers and improving SNP detection using self-avoiding molecular recognition systems [0.03%]
利用自我规避分子识别系统消除引物二聚体并提高单核苷酸多态性的检测能力
Zunyi Yang,Jennifer T Le,Daniel Hutter et al.
Zunyi Yang et al.
Despite its widespread value to molecular biology, the polymerase chain reaction (PCR) encounters modes that unproductively consume PCR resources and prevent clean signals, especially when high sensitivity, high SNP discrimination, and high...
Genome editing in the nematode Caenorhabditis briggsae using the CRISPR/Cas9 system [0.03%]
CRISPR/Cas9系统在秀丽隐杆线虫Caenorhabditis briggsae基因组编辑中的应用
Elizabeth Culp,Cory Richman,Devika Sharanya et al.
Elizabeth Culp et al.
The CRISPR/Cas system has recently emerged as a powerful tool to engineer the genome of an organism. The system is adopted from bacteria where it confers immunity against invading foreign DNA. This work reports the first successful use of t...
Erratum: PrimerPooler: automated primer pooling to prepare library for targeted sequencing [0.03%]
刊文更正：PrimerPooler：准备靶向测序库的自动引物混合器
Silas S Brown,Yun-Wen Chen,Ming Wang et al.
Silas S Brown et al.
[This corrects the article DOI: 10.1093/biomethods/bpx006.][This corrects the article DOI: 10.1093/biomethods/bpx006.]. © The Author(s) 2019. Published ...
Published Erratum
Biology methods & protocols. 2019 Nov 5;4(1):bpz015. DOI:10.1093/biomethods/bpz015 2019
Michael A Reeve,Denise Bachmann
Michael A Reeve
Analytical techniques currently available for the characterization of mixtures of microorganisms are generally based on next-generation sequencing. Motivated to develop practical and less-expensive methods for characterizing such mixtures, ...
Monica Chagoyen,Juan A G Ranea,Florencio Pazos
Monica Chagoyen
Due to the large interdependence between the molecular components of living systems, many phenomena, including those related to pathologies, cannot be explained in terms of a single gene or a small number of genes. Molecular networks, repre...