Single well, single-common primer pair, dual probe, duplex qPCR assay for the quantification of mRNA splicing variants [0.03%]
单井、单通用引物对、双探针、双重qPCR测定法用于量化mRNA剪接变体
Janice Wang,Winifred P Wong,Emma O Link et al.
Janice Wang et al.
Quantifying the ratio of alternatively spliced mRNA variants of genes with known alternative splicing variants is highly relevant for many applications. Herein, we describe the validation of a quantitative PCR design for the simplified quan...
Intestinal crypt-derived enteroid coculture in presence of peristaltic longitudinal muscle myenteric plexus [0.03%]
存在蠕动纵向肌肉肌间神经丛的肠隐窝源性类器官共培养
Daniel E Levin,Arabinda Mandal,Mark A Fleming et al.
Daniel E Levin et al.
The role of enteric neurons in driving intestinal peristalsis has been known for over a century. However, in recent decades, scientists have begun to unravel additional complex interactions between this nerve plexus and other cell populatio...
Bright fluorescent nucleic acid detection with CRISPR-Cas12a and poly(thymine) templated copper nanoparticles [0.03%]
基于CRISPR-Cas12a和聚胸腺嘧啶铜纳米粒子模板聚合的荧光检核酸检测新技术
Janna F M Bogers,Nicole F Berghuis,Ruud W Busker et al.
Janna F M Bogers et al.
Fluorescence-based diagnostic tools are attractive and versatile tests with multiple advantages: ease of use, sensitivity and rapid results. The advent of CRISPR-Cas technology has created new avenues for the development of diagnostic testi...
Rapid and real-time identification of fungi up to species level with long amplicon nanopore sequencing from clinical samples [0.03%]
基于临床样本的长扩增子纳米孔测序技术实现快速实时真菌鉴定至种水平
Sara D&#x;Andreano,Anna Cuscó,Olga Francino
Sara D&#x;Andreano
The availability of long-read technologies, like Oxford Nanopore Technologies, provides the opportunity to sequence longer fragments of the fungal ribosomal operon, up to 6 Kb (18S-ITS1-5.8S-ITS2-28S) and to improve the taxonomy assignment ...
Development of a fixation-free fluorescence in situ hybridization for the detection of Salmonella species [0.03%]
一种用于检测沙门氏菌属的无需固定切片的荧光原位杂交技术的发展
Oluwawemimo Adebowale,Liam Good
Oluwawemimo Adebowale
Salmonella is one of the most important infectious bacteria causing severe gastroenteritis and deaths in humans and animals, and the prompt diagnosis is crucial for effective control and treatment. The detection of Salmonella still depends ...
Concomitant prediction of environmental fate and toxicity of chemical compounds [0.03%]
同时预测化学物质的环境命运和毒性
Juan Antonio Garcia-Martin,Max Chavarría,Victor de Lorenzo et al.
Juan Antonio Garcia-Martin et al.
The environmental fate of many functional molecules that are produced on a large scale as precursors or as additives to specialty goods (plastics, fibers, construction materials, etc.), let alone those synthesized by the pharmaceutical indu...
ShinyButchR: Interactive NMF-based decomposition workflow of genome-scale datasets [0.03%]
ShinyButchR：基于NMF的大规模基因组数据集的交互式分解工作流
Andres Quintero,Daniel Hübschmann,Nils Kurzawa et al.
Andres Quintero et al.
Non-negative matrix factorization (NMF) has been widely used for the analysis of genomic data to perform feature extraction and signature identification due to the interpretability of the decomposed signatures. However, running a basic NMF ...
Positive/negative ion-switching-based LC-MS/MS method for quantification of cytosine derivatives produced by the TET-family 5-methylcytosine dioxygenases [0.03%]
基于正负离子切换的LC-MS/MS法定量TET家族5-甲基胞嘧啶双加氧酶产生的胞嘧啶衍生物
Aninda Sundar Dey,Navid J Ayon,Chayan Bhattacharya et al.
Aninda Sundar Dey et al.
Cytosine methylation at carbon-5 (5mC) in DNA plays crucial roles in epigenetic transcriptional regulation during metazoan development. The iron (II), 2-oxoglutarate-dependent Ten-Eleven Translocation (TET)-family dioxygenases initiate acti...
Lawrence Sirovich
Lawrence Sirovich
Many gene array studies of the yeast cell cycle have been performed (Cho RJ, Campbell MJ, Winzeler EA et al. A genome-wide transcriptional analysis of the mitotic cell cycle. Mol Cell 1998;2:65-73; Orlando DA, Lin CY, Bernard A et al. Globa...
Allowen Evin,Vincent Bonhomme,Julien Claude
Allowen Evin
Quantifying phenotypes is a common practice for addressing questions regarding morphological variation. The time dedicated to data acquisition can vary greatly depending on methods and on the required quantity of information. Optimizing dig...