Effect of nutritional education intervention to reduce anaemia during pregnancy in Dodoma City, Tanzania: protocol for a cluster randomized controlled trial [0.03%]
坦桑尼亚多多马市干预孕产妇缺铁性贫血营养教育项目效果集群随机对照试验方案
Mariam J Munyogwa,Nyasiro S Gibore,Agatha F Ngowi et al.
Mariam J Munyogwa et al.
The objective of this study is to assess the effectiveness of community-based nutritional intervention in reducing the burden of anaemia during pregnancy. Study design will be a cluster-randomized controlled trial. Study setting will be per...
Development of a LAMP assay using a portable device for the real-time detection of cotton leaf curl disease in field conditions [0.03%]
便携式LAMP设备现场检测棉花卷叶病毒的新方法研发
Amna Rafiq,Waqas Rafique Ali,Muhammad Asif et al.
Amna Rafiq et al.
Cotton production is seriously affected by the prevalent cotton leaf curl disease (CLCuD) that originated from Nigeria (Africa) to various parts of Asia including Pakistan, India, China and Philippines. Due to CLCuD, Pakistan suffers heavy ...
Sequence-specific inhibition of reverse transcription by recombinant CRISPR/dCas13a ribonucleoprotein complexes in vitro [0.03%]
体外通过重组CRISPR / dCas13a 核糖核蛋白复合物对逆向转录的序特异性抑制
Toshitsugu Fujita,Shoko Nagata,Miyuki Yuno et al.
Toshitsugu Fujita et al.
The clustered regularly interspaced short palindromic repeats (CRISPR) system is widely used for genome editing because of its ability to cleave specific DNA sequences. Recently, RNA-specific CRISPR systems have been reported. CRISPR system...
MINTyper: an outbreak-detection method for accurate and rapid SNP typing of clonal clusters with noisy long reads [0.03%]
MINTyper：一种用于克隆簇准确而快速的SNP分型和爆发检测方法，适用于带噪声的长读段
Malte B Hallgren,Søren Overballe-Petersen,Ole Lund et al.
Malte B Hallgren et al.
For detection of clonal outbreaks in clinical settings, we present a complete pipeline that generates a single-nucleotide polymorphisms-distance matrix from a set of sequencing reads. Importantly, the program is able to handle a separate mi...
A two-step PCR assembly for construction of gene variants across large mutational distances [0.03%]
一种两步PCR组装法，在较大突变距离下构建基因变异型
Shreya Routh,Anamika Acharyya,Riddhiman Dhar
Shreya Routh
Construction of empirical fitness landscapes has transformed our understanding of genotype-phenotype relationships across genes. However, most empirical fitness landscapes have been constrained to the local genotype neighbourhood of a gene ...
Hirotaka Matsumoto,Takahiro Mimori,Tsukasa Fukunaga
Hirotaka Matsumoto
Advances in experimental technologies, such as DNA sequencing, have opened up new avenues for the applications of phylogenetic methods to various fields beyond their traditional application in evolutionary investigations, extending to the f...
Circular DNA enrichment sequencing reveals the viral/satellites genetic diversity associated with the third epidemic of cotton leaf curl disease [0.03%]
基于圆形DNA捕获测序揭示棉叶卷曲病第三次大流行的病毒/卫星的基因多样性
Nasim Ahmed,Imran Amin,Syed Shan-E-Ali Zaidi et al.
Nasim Ahmed et al.
Cotton leaf curl disease (CLCuD) is the most important limiting factor for cotton production in Pakistan. The CLCuD passed through two major epidemics in this region with distinct begomoviruses/satellites complexes. Since 2015 the disease h...
Heterozygous KCNH2 variant phenotyping using Flp-In HEK293 and high-throughput automated patch clamp electrophysiology [0.03%]
用于Flp-In HEK293和高通量自动膜片钳电生理的KCNH2杂合变异表型分析
Chai-Ann Ng,Jessica Farr,Paul Young et al.
Chai-Ann Ng et al.
KCNH2 is one of the 59 medically actionable genes recommended by the American College of Medical Genetics for reporting of incidental findings from clinical genomic sequencing. However, half of the reported KCNH2 variants in the ClinVar dat...
Fluorescent-dependent comparative Ct method for qPCR gene expression analysis in IVF clinical pre-implantation embryonic testing [0.03%]
体外受精临床胚胎植入前基因表达分析的荧光依赖性比较Ct值法-qPCR检测技术
Arnav Lal,William E Roudebush,Monica Mainigi et al.
Arnav Lal et al.
The use of quantitative PCR (qPCR) and other polymerase chain reaction (PCR)-based methods in the field of human in vitro fertilization blastocoel fluid analysis can potentially be utilized for assisting clinicians in embryo selection based...
Single-tube genotyping for small insertion/deletion mutations: simultaneous identification of wild type, mutant and heterozygous alleles [0.03%]
单管基因分型法在小的插入/缺失突变中的应用：野生株、突变纯合子和杂合子的同时鉴定
Bin Lin,Jing Sun,Iain D C Fraser
Bin Lin
Current methods of genotyping small insertion/deletion (indel) mutations are costly, laborious, and can be unreliable. To address this, we have developed a method for small indel genotyping in a single polymerase chain reaction, with wild-t...