Positive/negative ion-switching-based LC-MS/MS method for quantification of cytosine derivatives produced by the TET-family 5-methylcytosine dioxygenases [0.03%]
基于正负离子切换的LC-MS/MS法定量TET家族5-甲基胞嘧啶双加氧酶产生的胞嘧啶衍生物
Aninda Sundar Dey,Navid J Ayon,Chayan Bhattacharya et al.
Aninda Sundar Dey et al.
Cytosine methylation at carbon-5 (5mC) in DNA plays crucial roles in epigenetic transcriptional regulation during metazoan development. The iron (II), 2-oxoglutarate-dependent Ten-Eleven Translocation (TET)-family dioxygenases initiate acti...
Lawrence Sirovich
Lawrence Sirovich
Many gene array studies of the yeast cell cycle have been performed (Cho RJ, Campbell MJ, Winzeler EA et al. A genome-wide transcriptional analysis of the mitotic cell cycle. Mol Cell 1998;2:65-73; Orlando DA, Lin CY, Bernard A et al. Globa...
Allowen Evin,Vincent Bonhomme,Julien Claude
Allowen Evin
Quantifying phenotypes is a common practice for addressing questions regarding morphological variation. The time dedicated to data acquisition can vary greatly depending on methods and on the required quantity of information. Optimizing dig...
Erratum to: Rapid, modular, and cost-effective generation of donor DNA constructs for CRISPR-based gene knock-in [0.03%]
图修正：快速、模块化和低成本的供体DNA构建物的生成用于基于CRISPR的基因敲入
Yi-Jiun Chen,Ya-Yun Cheng,Weikang Wang et al.
Yi-Jiun Chen et al.
[This corrects the article DOI: 10.1093/biomethods/bpaa006.]. © The Author(s) 2020. Published by Oxford University Press.
Published Erratum
Biology methods & protocols. 2020 Nov 18;5(1):bpaa021. DOI:10.1093/biomethods/bpaa021 2020
A lab in the field: applications of real-time, in situ metagenomic sequencing [0.03%]
现场中的实验室：实时原位元基因体定序之应用
Adriel Latorre-Pérez,Javier Pascual,Manuel Porcar et al.
Adriel Latorre-Pérez et al.
High-throughput metagenomic sequencing is considered one of the main technologies fostering the development of microbial ecology. Widely used second-generation sequencers have enabled the analysis of extremely diverse microbial communities,...
Uday Kiran,C G Gokulan,Santosh Kumar Kuncha et al.
Uday Kiran et al.
Rigorous testing is the way forward to fight the coronavirus disease 2019 pandemic. Here we show that the currently used and most reliable reverse transcription-polymerase chain reaction-based severe acute respiratory syndrome-coronavirus-2...
Characterization of FFPE-induced bacterial DNA damage and development of a repair method [0.03%]
福尔马林固定石蜡包埋诱导的细菌DNA损伤特征及修复方法的开发
Yensi Flores Bueso,Sidney P Walker,Mark Tangney
Yensi Flores Bueso
Formalin-fixed, paraffin-embedded (FFPE) specimens have huge potential as source material in the field of human microbiome research. However, the effects of FFPE processing on bacterial DNA remain uncharacterized. Any effects are relevant f...
Rapid and inexpensive whole-genome sequencing of SARS-CoV-2 using 1200 bp tiled amplicons and Oxford Nanopore Rapid Barcoding [0.03%]
使用1200 bp 瓷砖扩增子和牛津纳米孔快速条形码进行SARS-CoV-2的快速且低成本的全基因组测序
Nikki E Freed,Markéta Vlková,Muhammad B Faisal et al.
Nikki E Freed et al.
Rapid and cost-efficient whole-genome sequencing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes coronavirus disease 2019, is critical for understanding viral transmission dynamics. Here we show that u...
Pharmacophore-driven identification of N-methyl-D-receptor antagonists as potent neuroprotective agents validated using in vivo studies [0.03%]
药效团驱动的N-甲基-D受体拮抗剂作为神经保护剂的鉴定，并通过体内研究进行验证
Mukta Sharma,Anupama Mittal,Aarti Singh et al.
Mukta Sharma et al.
Alzheimer's disease (AD), apparently the most widespread reason behind dementia, is delineated by a continuous cognitive weakening in the aged. During its progression, N-methyl-D-aspartate receptor (NMDAR) antagonists are known to play a pi...
Method combining BAC film and positive staining for the characterization of DNA intermediates by dark-field electron microscopy [0.03%]
结合BAC薄膜和正染色技术通过暗场电子显微镜对DNA中间体进行表征的方法
Yann Benureau,Eliana Moreira Tavares,Ali-Akbar Muhammad et al.
Yann Benureau et al.
DNA intermediate structures are formed in all major pathways of DNA metabolism. Transmission electron microscopy (TEM) is a tool of choice to study their choreography and has led to major advances in the understanding of these mechanisms, p...