MultiPep: a hierarchical deep learning approach for multi-label classification of peptide bioactivities [0.03%]
多标签肽生物活性分类的层次深度学习方法
Alexander G B Grønning,Tim Kacprowski,Camilla Schéele
Alexander G B Grønning
Peptide-based therapeutics are here to stay and will prosper in the future. A key step in identifying novel peptide-drugs is the determination of their bioactivities. Recent advances in peptidomics screening approaches hold promise as a str...
Effect of community-based lifestyle education intervention to reduce cardiovascular diseases risk factors among vulnerable population in Dodoma city, Tanzania: a cluster randomized controlled trial study protocol [0.03%]
在坦桑尼亚Dodoma市易感人群中实施基于社区的生活方式教育干预以降低心血管疾病危险因素：聚类随机对照试验研究方案
Nyasiro S Gibore,Mariam J Munyogwa,Secilia K Ng&#x;weshemi et al.
Nyasiro S Gibore et al.
Tanzania is experiencing the rise of cardiovascular diseases (CVDs) and associated risk factors including hypertension, obesity and diabetes mellitus. Health education and healthy lifestyle promotion is an effective approach toward primary ...
Wildlife pathogen detection: evaluation of alternative DNA extraction protocols [0.03%]
野生动物病原体检测：评估替代DNA提取方案
Vasiliki Mantzana-Oikonomaki,Martine Maan,Joana Sabino-Pinto
Vasiliki Mantzana-Oikonomaki
Accurate detection of wildlife pathogens is critical in wildlife disease research. False negatives or positives can have catastrophic consequences for conservation and disease-mitigation decisions. Quantitative polymerase chain reaction is ...
OptM: estimating the optimal number of migration edges on population trees using Treemix [0.03%]
OptM：使用Treemix在种群树上估计最佳的迁移边数量
Robert R Fitak
Robert R Fitak
The software Treemix has become extensively used to estimate the number of migration events, or edges (m), on population trees from genome-wide allele frequency data. However, the appropriate number of edges to include remains unclear. Here...
Disha Sharma,Paras Sehgal,Sridhar Sivasubbu et al.
Disha Sharma et al.
Circular RNAs (circRNAs) are a novel class of noncoding RNAs that back-splice from 5' donor site and 3' acceptor sites to form a circular structure. A number of circRNAs have been discovered in model organisms including human, mouse, Drosop...
A comparison of the efficiency of RNA extraction from extracellular vesicles using the Qiagen RNeasy MinElute versus Enzymax LLC RNA Tini Spin columns and qPCR of miRNA [0.03%]
Qiagen RNeasy MinElute和Enzymax LLC RNA Tini Spin柱从细胞外囊泡中提取RNA的效率比较以及miRNA的qPCR检测
Rachael Anne Dunlop,Sandra Anne Banack,Paul Alan Cox
Rachael Anne Dunlop
One consequence of the current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is an interruption to the supply of laboratory consumables, particularly those used for RNA extraction. This category includes column-based...
MSCV-based retroviral plasmids expressing 3xFLAG-Sp-dCas9 for enChIP analysis [0.03%]
用于enCHIP分析的基于MSCV载体的慢病毒文库表达质粒(3xFLAG-Sp-dCas9)
Miyuki Yuno,Shoko Nagata,Toshitsugu Fujita et al.
Miyuki Yuno et al.
Engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) is a technology for purifying specific genomic regions to facilitate identification of their associated molecules, including proteins, RNAs, and other genomic r...
Optimization and biological validation of an in vitro assay using the transfected Dm28c/pLacZ Trypanosoma cruzi strain [0.03%]
一种用于Dm28c/pLacZ转基因T.克氏锥虫菌株的体外试验体系的优化及其生物学验证
Julián Ernesto Nicolás Gulin,Daniela Marisa Rocco,Victoria Alonso et al.
Julián Ernesto Nicolás Gulin et al.
There is an urgent need to develop safer and more effective drugs for Chagas disease, as the current treatment relies on benznidazole (BZ) and nifurtimox (NFX). Using the Trypanosoma cruzi Dm28c strain genetically engineered to express the ...
Quantifying the invasion and migration ability of cancer cells with a 3D Matrigel drop invasion assay [0.03%]
定量测量癌细胞在三维基质胶滴侵袭实验中的迁移和侵袭能力
Merve Aslan,En-Chi Hsu,Shiqin Liu et al.
Merve Aslan et al.
Metastasis is the main cause of cancer-associated morbidity which will account for ∼ 600,000 deaths in the USA in 2021. Defining new mechanisms that drive cancer metastasis is vital for developing new therapeutic strategies and improving c...
Cryopreservation of clonal and polyclonal populations of Chlamydomonas reinhardtii [0.03%]
裂殖壶藻无性繁殖群体的冷冻保存
Jacob Boswell,Charles Ross Lindsey,Emily Cook et al.
Jacob Boswell et al.
Long-term preservation of laboratory strains of Chlamydomonas reinhardtii has historically involved either liquid nitrogen cryopreservation, which is expensive and labor intensive, or storage on agar plates, which requires frequent transfer...