Quantifying Lysosomal Degradation of Extracellular Proteins With a Fluorescent Protein-Based Internalization Assay [0.03%]
利用荧光蛋白内吞试验量化溶酶体对细胞外蛋白质的降解
Sayana Bun,Kanna Kamikawa,Akira Matsuura et al.
Sayana Bun et al.
Endocytosis is an essential membrane transport mechanism that is indispensable for the maintenance of life. It is responsible for the selective internalization and subsequent degradation or recycling of specific extracellular proteins and n...
Simple Induction and Detection of Anthocyanins in Arabidopsis thaliana: A Tool for Mutant Screening and Metabolic Analysis [0.03%]
拟南芥中原花青素的简易诱导与检测：一种突变体筛选和代谢分析的方法
Yanli He,Samantha Iha,Erich Grotewold et al.
Yanli He et al.
Anthocyanins are specialized flavonoid pigments that play critical roles in plant coloration, photoprotection, and responses to environmental stress. Arabidopsis thaliana serves as a valuable genetic model for dissecting anthocyanin biosynt...
Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants [0.03%]
人前列腺肿瘤移植体的非酶法分离癌相关成纤维细胞
Giulia Gangarossa,Caterina Grillo,Sara Roccabianca et al.
Giulia Gangarossa et al.
Prostate carcinoma (PCa) progression is strongly influenced by the surrounding tumor microenvironment, where cancer-associated fibroblasts (CAFs) represent the most abundant and functionally relevant stromal population. Despite their import...
Dynamic Mapping of RNA-Binding Proteins During Bacillus subtilis Sporulation Using Orthogonal Organic Phase Separation [0.03%]
用于芽孢杆菌孢子形成过程中RNA结合蛋白动态图谱的正交有机相分离技术
Thomas Kaboré,Clémentine Delan-Forino
Thomas Kaboré
RNA-binding proteins (RBPs) have pleiotropic roles in modulating the physiology of both eukaryotic and prokaryotic cells, enabling them to adapt to environmental variations. The importance of RBPs has led to the development of a variety of ...
Miquel Sendra,Adriano Bolondi,Léo Guignard
Miquel Sendra
Serial spatial omics technologies capture genome-wide gene expression patterns in thin tissue sections but lose spatial continuity along the third dimension. Reconstructing these two-dimensional measurements into coherent three-dimensional ...
Identification of the Subcompartment-Specific Mitochondrial Proteome by APEX2 Proximity Labeling in Saccharomyces cerevisiae [0.03%]
APEX2邻近标记技术识别酿酒酵母线粒体内膜和嵴腔蛋白组
Lorenz Spänle,Johannes M Herrmann
Lorenz Spänle
The cellular compartments of eukaryotic cells are defined by their specific protein compositions. Different strategies are used for the identification of the subcellular proteomes, such as fractionation by differential centrifugation of cel...
Anish J Virdi,Ajit P Joglekar
Anish J Virdi
The deep learning revolution has accelerated discovery in cell biology by allowing researchers to outsource their microscopy analyses to a new class of tools called cell segmentation models. The performance of these models, however, is ofte...
In Ovo CAM-Based Xenograft Model for Investigating Tumor Developmental Biology in Breast Cancer [0.03%]
基于乳腺癌肿瘤发生发育生物学的卵内CAM系模型研究
Carlos César Patiño Morales,Claudia Haydée González de La Rosa,Ricardo Jaime-Cruz et al.
Carlos César Patiño Morales et al.
Breast cancer remains one of the most prevalent and deadly malignancies affecting women worldwide. Its progression and metastatic behavior are driven by complex mechanisms. To develop more effective therapeutic strategies, it is crucial to ...
Quick and Cheap: Optimized Purification and Concentration of Bacteriophages Produced in Rich Culture Media [0.03%]
快速且廉价的富培养基菌噬液浓缩纯化新方法优化及评估研究
Amel Chaïb,Laura Schmitt,Adeline Goulet et al.
Amel Chaïb et al.
This protocol describes an easy, quick, cheap, and effective method for the purification and concentration of bacteriophages (phages) produced in rich culture media, meeting the quality criteria required for structural analyses. It is based...
Orthogonal Temperature-Related Intensity Change and Time-Resolved Förster Resonance Energy Transfer High-Throughput Screening Platform for the Discovery of SLIT2 Binders [0.03%]
正交的温度相关的强度变化和时间分辨的Förster共振能量转移高通量筛选平台用于SLIT2结合分子的发现
Nelson García-Vázquez,Somaya A Abdel-Rahman,Moustafa T Gabr
Nelson García-Vázquez
SLIT2 is a secreted glycoprotein implicated in axon guidance, immune modulation, and tumor biology, whose extracellular and glycosylated nature can complicate conventional biophysical screening workflows. Here, we provide a complete, step-b...