Quantitative Analysis of the Arabidopsis Leaf Secretory Proteome via TMT-Based Mass Spectrometry [0.03%]
基于TMT质谱的拟南芥叶片分泌蛋白组的定量分析
Sakharam Waghmare,Lingfeng Xia,Suzanne McGill et al.
Sakharam Waghmare et al.
In plants, the apoplast contains a diverse set of proteins that underpin mechanisms for maintaining cell homeostasis, cell wall remodeling, cell signaling, and pathogen defense. Apoplast protein composition is highly regulated, primarily th...
Monitoring Endocytosis of Integral Membrane Proteins Using Western Blot-Based Detection of Biotinylated Antibody Uptake [0.03%]
采用生物素标记抗体摄取的Western印迹检测法监测整合膜蛋白内吞作用
Alexandra Graninger,Prasanna Satpute-Krishnan
Alexandra Graninger
The antibody-uptake assay is a commonly used technique to monitor endocytosis of integral membrane proteins including transmembrane and glycosylphosphatidylinositol-anchored proteins (GPI-APs). The antibody-uptake assay typically involves i...
Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood [0.03%]
人冷冻保存的PBMC和全血中的JAK/STAT信号通路比较分析的双重磷酸化CyTOF工作流程
Ilyssa E Ramos,Brynja Matthiasardottir,Teresa S Hawley et al.
Ilyssa E Ramos et al.
Protein phosphorylation is a dynamic post-translational modification that regulates fundamental processes, including signal transduction, cell proliferation, differentiation, and effector function of immune cells. The Janus Kinase/Signal Tr...
Mouse Vestibulo-Ocular Reflex Testing for Otolith Organs and Horizontal Semicircular Canal [0.03%]
小鼠前庭眼动反射试验在膜迷路病变中的应用及优缺点评估
Tong Zhao,Shijie Xiao,Qingsong Liu et al.
Tong Zhao et al.
Vestibulo-ocular reflexes (VORs) are compensatory ocular reflexes that maintain stable vision during head movements. In research, VORs encompass angular VOR (aVOR) and off-vertical axis rotation (OVAR) tests, which various groups have emplo...
In Vivo Retroviral Transduction of Cardiac Myofibroblasts Using Intramyocardial Injection Immediately Post-myocardial Infarction [0.03%]
心肌梗塞后即刻进行心内注射对心肌纤维母细胞的体内逆转录病毒转导作用
Satsuki Ono,Hayato Watanabe,Yuma Horii et al.
Satsuki Ono et al.
Following myocardial infarction (MI), myocardial cells undergo cell death, and the necrotic region is replaced by extracellular matrix (ECM) proteins such as collagens. Myofibroblasts are responsible for producing these ECM proteins. Cardia...
Immunopeptidomics Workflow for Isolation and LC-MS/MS Analysis of MHC Class I-Bound Peptides Under Hypoxic Conditions [0.03%]
缺氧条件下MHC I类分子肽类抗原的免疫蛋白组分析新方法
Hala Estephan,Ester M Hammond,Eleni Adamopoulou
Hala Estephan
Immunopeptidomics enables the identification of peptides presented by major histocompatibility complex (MHC) molecules, offering insights into antigen presentation and immune recognition. Understanding these mechanisms in hypoxic conditions...
Characterizing Tissue Oxygen Tension During Neurogenesis in Human Cerebral Organoids [0.03%]
人脑类器官神经发生过程中组织氧张力的特征研究
Yuan-Hsuan Liu,Hsiao-Mei Wu
Yuan-Hsuan Liu
Oxygen tension is a key regulator of early human neurogenesis; however, quantifying intra-tissue O2 in 3D models for an extended period remains difficult. Existing approaches, such as needle-type fiber microsensors and intensity-based oxyge...
Spheroid Sheets: A Scalable Platform for Producing Tissue Membrane Constructs [0.03%]
类器官片层：生产组织膜构件的可拓展平台
Quang Bach Le,Hariharan Ezhilarasu,Weng Wan Chan et al.
Quang Bach Le et al.
Bottom-up tissue engineering using cell spheroids offers many advantages in recapitulating native cell-cell and cell-matrix interactions. Many tissues, such as cartilage, bone, cardiac muscle, intestine, and neural tissues, have been tissue...
Rapid and Solvent-Free, 2-hydroxyethyl Methacrylate (HEMA)-Acrylamide (AAm) Copolymer-Based Optical Clearing of Tissue for Fluorescent Imaging [0.03%]
基于2-羟乙基甲基丙烯酸酯(PHEMA)-丙烯酰胺(AAm)共聚物的组织快速无溶剂光学透明化以用于荧光成像
Yanran Wang,Siying Feng,Xiaoqi Zhou et al.
Yanran Wang et al.
The study of whole organs or tissues and their cellular components and structures has been historically limited by their natural opacity, which is caused by the optical heterogeneity of the tissue components that scatter light as it travers...
A Step-by-Step Computational Protocol for Functional Annotation and Structural Modelling of Insect Chemosensory Proteins [0.03%]
昆虫化学感觉蛋白的功能注释和结构建模的逐步计算协议
Rajeswari Kalepu,Azzmer Azzar Abdul Hamid,Maizom Hassan et al.
Rajeswari Kalepu et al.
Insects rely on chemosensory proteins, including gustatory receptors, to detect chemical cues that regulate feeding, mating, and oviposition behaviours. Conventional approaches for studying these proteins are limited by the scarcity of expe...