Isolation of Antigen-Specific Nanobodies From Synthetic Libraries Using a Protein Selection Strategy That Combines MACS-Based Screening of YSD and FLI-TRAP [0.03%]
基于MACS的YSD筛选和FLI-TRAP结合的蛋白质选择策略从合成库中分离抗原特异性纳米体
Apisitt Thaiprayoon,Yodpong Chantarasorn,Worrapoj Oonanant et al.
Apisitt Thaiprayoon et al.
Although protein-protein interactions (PPIs) are central to nearly all biological processes, identifying and engineering high-affinity intracellular binders remains a significant challenge due to the complexity of the cellular environment a...
Assessing the Toxoplasma Tachyzoite Cell Cycle Phases Using Fluorescent Ubiquitination-Based Cell Cycle Indicator [0.03%]
基于荧光泛素化的细胞周期指示器评估速殖子的胞内增殖周期阶段
Mrinalini Batra,Elena S Suvorova
Mrinalini Batra
Toxoplasma gondii is an apicomplexan parasite that infects a wide variety of eukaryotic hosts and causes toxoplasmosis. The cell cycle of T. gondii exhibits a distinct architecture and regulation that differ significantly from those observe...
Melissa Cuevas,Kenneth Jones,Nancy Hadley Miller
Melissa Cuevas
It is common practice for laboratories to discard clotted blood or freeze it for future DNA extraction after extracting serum from a serum-separating tube. If freezing for DNA extraction, the blood clot is not usually cryopreserved, which l...
Electroporation of Whole-Mount Postnatal Rodent Retinas for Advanced Functional Assays [0.03%]
电穿孔法应用于整装的产后啮齿类动物视网膜以进行高级功能检测
Chien-Ting Huang,Tzu-Jen Chen,Yu-Lin Su et al.
Chien-Ting Huang et al.
To study gene function in regulating rodent retinal waves during development, an efficient method for gene delivery into whole-mount retinas is required while preserving circuit functionality for physiological studies. We present an optimiz...
Correcting Image Distortion in Expansion Microscopy Using 3D-Aligner [0.03%]
基于3D-aligner的膨胀显微影像几何失真的校正方法
Wan-Yi Hsiao,Dhaval Ghone,Aussie Suzuki
Wan-Yi Hsiao
Expansion microscopy (ExM) is an innovative and cost-effective super-resolution imaging technique that enables nanoscale visualization of biological structures using conventional fluorescence microscopes. By physically enlarging biological ...
A Reproducible Method to Evaluate Sublethal Acoustic Stress in Aquatic Invertebrates Using Oxidative Biomarkers [0.03%]
基于氧化型生物标志物评估水生无脊椎动物亚致死声学压力的可重复方法
Francesca Maria Mitton,Solana Morena Snitman,Maria Ceraulo et al.
Francesca Maria Mitton et al.
Underwater noise is a growing source of anthropogenic pollution in aquatic environments. However, few studies have evaluated the impact of underwater noise on aquatic invertebrates. More importantly, studies involving early developmental st...
A Simple Protocol for Periodic Live Cell Observation of Flagellate Stages in the Lichen Alga Trebouxia [0.03%]
一种对地衣藻类Trebouxia鞭毛体阶段进行定期活细胞观察的简便方法
Enrico Boccato,Fabio Candotto Carniel,Mauro Tretiach
Enrico Boccato
Flagellate stages of green microalgae such as Trebouxia are only partially characterised, with recent evidence suggesting that they are involved in both sexual and asexual reproduction. Conventional methods based on fixed samples in light, ...
Optimization of Adipogenic Differentiation Protocol for Murine and Human Cell Culture Models [0.03%]
小鼠和人类细胞培养模型的脂肪分化方案优化
Junwan Fan,Ling Zhou,Yingjie Geng et al.
Junwan Fan et al.
Adipogenic differentiation efficiency remains highly variable across laboratories and cellular models, underscoring a critical need for a robust and standardized protocol. Here, we describe an optimized and highly effective protocol for ind...
Plasmodium berghei High-Throughput (PbHiT): a CRISPR-Cas9 System to Study Genes at Scale [0.03%]
伯氏疟原虫高通量（PbHiT）系统：一种大规模研究基因的CRISPR-Cas9系统
Thorey K Jonsdottir,Martina S Paoletta,Johan Henriksson et al.
Thorey K Jonsdottir et al.
Genetic modification is essential for understanding parasite biology, yet it remains challenging in Plasmodium. This is partially due to the parasite's low genetic tractability and reliance on homologous recombination, since the parasites l...
Peng Chen,Shen Zhou,Haonan Wang et al.
Peng Chen et al.
Reduced representation sequencing (RRS), particularly through restriction site-associated DNA sequencing (RAD-seq), has been widely adopted for whole-genome genotyping due to its cost-effectiveness and cross-species applicability. Neverthel...