Using Single-Particle Fluorescence Microscopy to Quantify Substrate Binding of Peptidoglycan-Modification Enzymes [0.03%]
利用单粒子荧光显微镜技术量化肽聚糖修饰酶的底物结合能力
Carlos Ramírez A Carbó,Beiyan Nan
Carlos Ramírez A Carbó
Peptidoglycan (PG), a network of glycan strands crosslinked by short peptides, is an essential and bacterial-specific structure that determines cell shape and protects cells from lysis. Understanding how bacteria assemble, maintain, and mod...
Isolation and Biophysical Characterization of Extracellular Vesicles Released by Myocytes [0.03%]
心肌细胞释放的外泌体的分离及生物物理表征
Kah Yong Goh,Wen Xing Lee,Hong-Wen Tang
Kah Yong Goh
Extracellular vesicles (EVs) are lipid bilayer-enclosed vesicles released by diverse cell types and found in various body fluids. Because their composition and cargo dynamically respond to physiological and environmental cues, EVs hold prom...
Quantification of Spatial Patterns of Microtubule Transport by Kinesin-1 Head and Tail [0.03%]
驱动蛋白I的头尾结构微管运输的空间分布量化研究
Jashaswi Basu,Kajal Singh,Anita Jannasch et al.
Jashaswi Basu et al.
The conventional kinesin-1 is a plus-end-directed microtubule-dependent motor protein with distinct motor head, stalk, and tail domains. Along with the motor head, which binds and walks along microtubules in an adenosine 5'-triphosphate (AT...
Analysis of Cauline Leaf Development in Arabidopsis thaliana Using Time-Lapse Confocal Microscopy [0.03%]
利用活体激光共聚焦显微镜分析拟南芥茎生叶发育
Constance Le Gloanec
Constance Le Gloanec
Understanding cellular growth dynamics in plants requires precise, long-term imaging of developing tissues. Cauline leaves are produced during the transition from vegetative to reproductive development and provide a useful system for studyi...
3D Reconstruction of Mature Arabidopsis Ovules Using FIB-SEM to Study Filiform Apparatus Morphology [0.03%]
应用聚焦离子束扫描电子显微镜技术对拟南芥成熟大孢子进行三维重建以研究细线体结构
Takao Oi,Tomoyo Morikawa,Yuna Yamazaki et al.
Takao Oi et al.
Volume electron microscopy based on serial sectioning allows for three-dimensional (3D) visualization and analysis of the internal structures of tissues, cells, and organelles. One such technique, focused ion beam (FIB) scanning electron mi...
A Step-by-Step GUI-Based Protocol for Molecular Dating Analysis Using PhyloSuite v2 [0.03%]
基于图形界面的分子 dating 分析软件 Phylosuite 的操作流程
Dong Zhao,Ivan Jakovlić,Xiantong Liu et al.
Dong Zhao et al.
In current genomic research, molecular dating is challenged by both imperfect substitution modeling and analysis efficiency, as genome-scale datasets often exhibit substantial rate heterogeneity and complex patterns of sequence evolution, w...
Plasmid Curing of Pseudoalteromonas haloplanktis TAC125 Using Homologous Recombination and PTasRNA Gene Silencing [0.03%]
利用同源重组和PTasRNA基因干扰沉默技术从Pseudoalteromonas haloplanktis TAC125中去除质粒
Angelica Severino,Concetta Lauro,Marzia Calvanese et al.
Angelica Severino et al.
Pseudoalteromonas haloplanktis TAC125 is a psychrophilic marine bacterium widely used to study cold adaptation and increasingly exploited as a non-conventional platform for biotechnological applications. The strain harbors the endogenous me...
Biolayer Interferometry (BLI) to Quantify RALF1-Pectin Interactions [0.03%]
利用生物层干涉光谱法(BLI)量化RALF1-果胶的相互作用
Susan Lauw,Elke Barbez
Susan Lauw
Cellular function relies on a network of precisely regulated interactions among macromolecules such as proteins, peptides, carbohydrates, and nucleic acids. These molecular interactions regulate vital processes, including signaling, structu...
PEPTERGENT: A Peptide-Based Reagent for Detergent-Free Extraction of Membrane Proteins and Purification of Membrane Proteomes [0.03%]
一种用于无去污剂膜蛋白提取及膜蛋白质组纯化的肽基试剂(PEPTERGENT)
Frank Antony,Ashim Bhattacharya,Franck Duong van Hoa
Frank Antony
Peptergent is a novel class of amphipathic peptides that enables detergent-free extraction of membrane proteins (MPs) from lipid bilayers. This reagent self-assembles around hydrophobic transmembrane regions, forming stable, water-soluble c...
An In Vitro A-431 Epithelial Cell Infection Model for Studying Fungal Pathogenicity and Immune Responses Associated With Vulvovaginal Candidiasis [0.03%]
外阴阴道念珠菌病相关致病性和免疫反应的体外A-431上皮细胞感染模型研究
Victoria E Groß,Kar On Cheng,Beatriz Cristóvão et al.
Victoria E Groß et al.
Vulvovaginal candidiasis (VVC), also known as vaginal thrush, is an infection of the vulvovaginal mucosa caused by fungi of the Candida genus. Particularly for patients suffering from recurrent infection, the disease has a significant impac...