Employing Tribe to Study RNA Interactions of Ataxin-2 in Drosophila S2 Cells [0.03%]
运用TRIBE技术研究Ataxin-2的RNA相互作用：果蝇S2细胞中的案例分析
Shiva Kumar,Omkar Koppaka,Khushboo Agrawal et al.
Shiva Kumar et al.
RNA-binding protein (RBP)-RNA interactions are fundamental for gene regulation and cellular homeostasis. Ataxin-2 is an RBP that has been shown to play an instrumental role in pathophysiological processes by binding to mRNA. Methods such as...
A Standardized Culture Medium for Comparative Drug Efficacy Evaluation Across Plasmodium and Babesia Species [0.03%]
一种标准化培养基用于毕氏疟原虫和巴贝斯虫属不同种类的药物疗效评价
Pratap Vydyam,Choukri Ben Mamoun
Pratap Vydyam
The discovery of broad-spectrum antiparasitic agents relies on the ability to evaluate drug efficacy under harmonized in vitro conditions across related species. However, current drug screening pipelines for intraerythrocytic parasites are ...
High-Resolution Quantification of Two-Way Nanobody Synergy Using Automated Liquid Handling and Computational Modeling [0.03%]
使用自动化液体处理和计算建模高分辨率量化双向纳米抗体协同作用
Natasha M Bourgeois,Satchel W Bell,Jean Paul Olivier et al.
Natasha M Bourgeois et al.
Evaluating single-domain antibody cooperativity is essential for developing potent, escape-resistant antiviral biologics. Here, we present a protocol that reproducibly quantifies functional synergy between neutralizing nanobody pairs in sta...
aGPCR-HEK: A Stable High-Expression Inducible Mammalian Cell Expression System for Adhesion GPCR Structural Biology Applications [0.03%]
用于粘附G蛋白偶联受体结构生物学应用的稳定高表达诱导型哺乳动物细胞表达系统
David M Favara,Christopher G Tate
David M Favara
ADGRL4 is an adhesion G protein-coupled receptor (aGPCR) implicated in multiple tumours. In our experience, conventional insect cell-based baculovirus expression systems have not yielded sufficient correctly folded ADGRL4 protein for purifi...
Development, Expansion, and Histological Characterization of Patient-Derived Liver Organoids for Drug Screening and Disease Modeling [0.03%]
基于患者衍生的肝器官芯片的药物筛选及疾病模型的构建和组织学分析
Silvia De Siervi,Stefania Mantovani,Barbara Oliviero et al.
Silvia De Siervi et al.
Organoids are self-organizing 3D tissues representing an innovative technology with interesting implications and potential for the study of tumor biology. They can be developed from fine-needle biopsies or resection material from healthy or...
Combining Suction-Pipette Spectral Identification With Single-Cell RT-PCR to Make Differential Analyses of Amphibian Red and Green Rods [0.03%]
结合抽吸光谱鉴定与单细胞RT-PCR技术对两栖类红绿视杆细胞进行差异分析
Olga V Chernyshkova,Darya A Nikolaeva,Darya A Meshalkina et al.
Olga V Chernyshkova et al.
Amphibian retinas contain "green" rods, which are rod-shaped photoreceptors with a cone-type visual pigment. These rods are considered a potentially transitional photoreceptor type, but their phototransduction cascade's molecular compositio...
Obtaining Chondroprogenitors (Articular Cartilage-Derived Cells) via Explant Methodology [0.03%]
利用 explants 方法获得软骨前体细胞（关节软骨来源细胞）
Débora Levy,Pedro Nogueira Giglio,Fabio Alessandro de Freitas et al.
Débora Levy et al.
Obtaining articular cartilage-derived cells (chondroprogenitors) by explant methodology is a reliable approach for isolating migratory progenitor cells that retain strong chondrogenic potential. This method allows cells to emerge naturally ...
Ritian Qin,Fuchu He,Weijie Qin
Ritian Qin
Spatial proteomics enables the mapping of protein distribution within tissues, which is crucial for understanding cellular functions in their native context. While spatial transcriptomics has seen rapid advancement, spatial proteomics faces...
Framework for Analyzing the Anti-biofilm and Anti-virulence Activities of Fatty Acids from Hermetia illucens Larvae Targeting Multidrug-Resistant Klebsiella pneumoniae [0.03%]
赫氏光辉杆虫幼虫脂肪酸针对多重耐药肺炎克雷伯菌抑制生物膜和减毒作用分析的框架
Heakal Mohamed,Elena Marusich,Sergey Leonov
Heakal Mohamed
The emergence of antimicrobial resistance and the persistence of Klebsiella pneumoniae biofilms represent significant challenges to public health. Hermetia illucens (HI) larvae are considered a sustainable reservoir of novel bioactive compo...
Selective Isolation of TOP3B•mRNA Covalent Intermediates Using Denaturing Oligo-dT Pulldown [0.03%]
使用变性寡聚dT进行TOP3B•mRNA共价中间体的选择性分离
Julia E Warrick,Michael G Kearse
Julia E Warrick
The deletion and mutation of Topoisomerase 3β (TOP3B) is linked to multiple neurological disorders and is the only known topoisomerase that is also catalytically active on RNA in vitro and in cells. Uniquely, TOP3B is primarily localized t...