Mag-Net Strong Anion Exchange Enables Isolation of Ovarian Cancer Ascites Extracellular Vesicles for Proteomic Biomarker Discovery [0.03%]
强阴离子交换使卵巢癌腹水外泌体的分离成为可能，用于蛋白质组生物标志物发现
Tyler T Cooper
Tyler T Cooper
Extracellular vesicles (EVs) are nanoscale particles secreted by all cells and present in all biological fluids, where they carry molecular cargo reflective of health and disease states. Their diagnostic potential is often obscured by the h...
Controlled Transmission of a Fijivirus Under Field Conditions Using Mass-Reared Planthoppers [0.03%]
利用大规模饲养的稻飞虱控制田间菲吉病毒的传播
Analía D Dumón,Milene R Barcenilla,Julieta B Bariles et al.
Analía D Dumón et al.
Mal de Río Cuarto disease, caused by a Fijivirus, is a major constraint for maize production in Argentina. The traditional evaluation of resistant hybrids is limited by the low efficiency of natural virus transmission and the lack of stand...
A Novel Sequencing Method for Quantification of ZIKV RNA in Individual Cells [0.03%]
一种新的测序方法 可量化单个细胞中的寨卡病毒rna
Min Hao,Yisong Wang,Dianyi Du et al.
Min Hao et al.
Single-cell RNA sequencing (scRNA-seq) is a powerful technique for exploring cellular heterogeneity and host-pathogen interactions. This protocol details the Zika virus (ZIKV)-targeted scRNA-seq workflow for preparing high-quality single-ce...
A Guide to Reproducible Cellulose Synthase Density and Speed Measurements in Arabidopsis thaliana [0.03%]
拟南芥细胞分裂素合成酶密度和速度测定指南
Jan Y Xue,Shawn D Mansfield,Lacey A Samuels et al.
Jan Y Xue et al.
Cellulose synthase complexes (CSCs) play a central role in plant cell wall formation. Their dynamic behavior at the plasma membrane leads to the deposition of cellulose microfibrils into the apoplastic space, thereby shaping the architectur...
A Simple and Cost-Effective Method for Generating Spheroids From Triple-Negative Breast Cancer Cell Line (MDA-MB-231) [0.03%]
一种简单且低成本的生成三阴性乳腺癌细胞系(MDA-MB-231)spheroid的方法
Ramón Cervantes-Rivera,Luisa Nirvana González-Fernández,Atalia Ziret Romero Rosas et al.
Ramón Cervantes-Rivera et al.
Breast cancer (BC) is the most frequently diagnosed malignancy in women and a leading cause of cancer-related mortality worldwide. Current clinical management relies on molecular classification-based on estrogen receptor (ER), progesterone ...
Fluorescence-Based Absent Allele-Specific Amplification (FAASA) for High-Throughput Detection of Absent Alleles [0.03%]
基于荧光的缺失等位基因特异性扩增(FAASA)技术用于高通量检测缺失等位基因
Katherine L D Running,Sudeshi Seneviratne,Zengcui Zhang et al.
Katherine L D Running et al.
In wheat and other crops, some genes display presence/absence variation, and it is occasionally beneficial to select for the absent allele to remove a functional gene. However, current high-throughput genotyping methods used to detect the a...
Reconstruction of Axonal Projections of Single Neurons Using PointTree [0.03%]
基于PointTree的单神经元轴突重构方法
Lin Cai,Xuzhong Qu,Junwei Wang et al.
Lin Cai et al.
The morphology of single-neuron axonal projections is critical for deciphering neural circuitry and information flow in the brain. Yet, manually reconstructing these complex, long-range projections from high-throughput whole-brain imaging d...
Alexander James Long,Diogo Candeias,Nicki Frederick Coveña et al.
Alexander James Long et al.
Organelle abundance is a key microscopic readout of organelle formation and, in many cases, function. Quantification of organelle abundance using confocal microscopy requires estimating their area based on the fluorescence intensity of comp...
A Rapid and High-Recovery Extracellular Vesicle (EVs) Isolation Technique from Blood Samples [0.03%]
一种从血液样本中快速高效分离外泌体(EVs)的技术
Aryan N Sankpal,Narendra V Sankpal
Aryan N Sankpal
Extracellular vesicles (EVs) circulating in blood serve as non-invasive "liquid biopsies," carrying molecular cargo that reflects the physiological and pathological state of distant cells. Their analysis is crucial for understanding disease...
Purification of the Active-State G Protein-Coupled Receptor ADGRL4 for Cryo-Electron Microscopy Using a Modular Tag System and a Tethered mini-Gq [0.03%]
使用模块化标签系统和连接的mini-Gq纯化活化状态的G蛋白偶联受体ADGRL4用于 cryo-EM分析
David M Favara,Christopher G Tate
David M Favara
ADGRL4 is an adhesion G protein-coupled receptor (aGPCR) implicated in tumour progression in multiple malignancies. We recently determined the first cryo-EM structure of active-state ADGRL4, revealing its weak coupling to the heterotrimeric...