Alexandra K Walker,Alexander J Lanz,Joanna R Morris
Alexandra K Walker
Small ubiquitin-related modifiers (SUMOs) are covalently conjugated onto the proteome and serve as signaling molecules in many aspects of eukaryotic cell biology, from S. cerevisiae and C. elegans to H. sapiens. The conjugatable SUMO varian...
Tomas Armani,Anabella R Nicolli,Lucia Zalazar et al.
Tomas Armani et al.
Extracellular vesicles (EVs) are critical mediators of cell-cell communication and play a key role in male reproductive biology by modulating sperm function. This protocol describes a robust and reproducible workflow for isolating EVs from ...
A Simple and Easy Method for RNA Extraction from the Cyanobacterium Synechocystis sp. PCC 6803 [0.03%]
一种从蓝藻鱼腥藻菌株PCC6803中提取RNA的简易方法
Bharat Kumar Majhi,Julian J Eaton-Rye
Bharat Kumar Majhi
Cyanobacteria have been widely used as model organisms in photobiochemical research and have recently been exploited as hosts in numerous pilot studies to produce valuable biochemicals via genetic and metabolic modifications. Analyzing cell...
A Male Mouse Model of WIN 55,212-2 Self-Administration to Study Cannabinoid Addiction [0.03%]
建立WIN 55212-2条件位置偏爱的雄性小鼠模型以研究大麻素类物质成瘾
Elena Martín-García,María Fernanda Ponce-Beti,Tatiana Gusinskaia et al.
Elena Martín-García et al.
Despite substantial progress in preclinical cannabinoid research, translational studies on cannabis use disorders (CUD) are still insufficient due to the absence of robust, validated animal models that fully recapitulate the multifactorial ...
3D STED Super-Resolution Imaging Strategy for Visualizing Synaptic Nano-architecture in Brain Cryosections [0.03%]
一种用于可视化脑切片突触纳米结构的3D STED超分辨率成像策略
James Scripter,Adam Skeens,Grace Jones et al.
James Scripter et al.
Super-resolution imaging of synapses in intact brain tissue remains challenging because light scattering, photobleaching, and limited probe penetration, along with antigen accessibility within the densely packed postsynaptic densities (PSDs...
A Cytosine Deaminase-Based Genomic Footprinting Assay (cFOOT-seq) for Detecting Transcription Factor Occupancy [0.03%]
一种基于胞苷脱氨酶的基因组足迹测定法（cFOOT-seq）用于检测转录因子占用率
Meng-Chen Yang,Ang Wu,Heng Wang et al.
Meng-Chen Yang et al.
Transcription factors (TFs) regulate gene expression by binding to cis-regulatory elements in the genome. Understanding transcriptional regulation requires genome-wide characterization of TF occupancy across different chromatin contexts, ye...
A Rapid and Visual Soybean Hairy Root Transformation Protocol Using the RUBY Reporter [0.03%]
一种使用RUBY报告基因的快速可视化大豆毛根转化协议
Zhirui Zhang,Qiushi Wang,Yu Geng et al.
Zhirui Zhang et al.
Agrobacterium rhizogenes-mediated hairy root transformation provides a rapid platform for gene function analysis prior to stable whole-plant transformation. However, most existing hairy root transformation methods rely on tissue culture and...
Tandem RNA and Protein Extraction: A Platform for Maximizing the Use of Limited Ex Vivo Tissue Samples [0.03%]
串联RNA和蛋白质提取：最大限度地利用有限的组织样本的平台
Ciarán Kennedy,Braden Millar,Luke J Conroy et al.
Ciarán Kennedy et al.
Human tissue samples represent the gold standard for obtaining clinically relevant and translatable insight into disease processes that in vitro systems cannot fully reproduce. However, patient-derived samples are often limited in size and ...
Alan Wainman
Alan Wainman
Centrosomes are dynamic organelles critical for mitotic spindle assembly and cilia formation. Here, I describe a protocol for quantifying relative centrosomal protein abundance in Drosophila melanogaster embryos using radial profile analysi...
A Bioinformatics Workflow to Identify eccDNA Using ECCFP From Long-Read Nanopore Sequencing Data [0.03%]
一种使用长读纳米孔测序数据识别eccDNA的生物信息学工作流ECCFP
Wang Li,Biyuan Miao,Shaogui Wan
Wang Li
Extrachromosomal circular DNA (eccDNA) is a type of circular DNA that exists independently of chromosomes and has garnered significant attention in various fields, particularly in the context of smaller eccDNAs, which have considerable role...