Spatial Imaging and Quantification of Hydrogen Peroxide in Arabidopsis Roots: From Sample Preparation to Image Analysis [0.03%]
基于成像的植物根系活性氧表型分析及定量研究：从样品制备到图像处理
Mario Fenech,Vitor Amorim-Silva
Mario Fenech
Reactive oxygen species (ROS) are central regulators of plant development and stress responses, with hydrogen peroxide (H2O2) acting as a key signaling molecule whose spatial distribution determines adaptive versus damaging outcomes. Accura...
Quantifying Epigenetic Changes Induced by Chemical Exposure Using the epi-TK Assay [0.03%]
采用表观遗传毒性TK基因 assays 测定化学物质暴露所致的 DNA 甲基化变化
Shiro Kuroki,Haruto Yamada,Mizuki Odagiri et al.
Shiro Kuroki et al.
Epigenetic modifications play essential roles in regulating gene expression and maintaining cellular identity. Accumulating evidence suggests that chemical agents can contribute to carcinogenesis through epigenetic alterations, such as chan...
Optical Control of Actin Network Assembly on the Supported Lipid Bilayer [0.03%]
光控制支撑脂质双层上的肌动蛋白网络组装
Kei Yamamoto,Makito Miyazaki
Kei Yamamoto
The spatiotemporal dynamics and density of actin networks are key determinants of actin cytoskeleton-mediated cellular functions. In vitro reconstitution systems have been widely used to study actin cytoskeletal dynamics; however, many exis...
Isolation, Culture, and Differentiation of Bovine Muscle Resident Stem Cells [0.03%]
牛肌肉基质干细胞的分离、培养及分化能力
Perri Gish,Madison W Stewart,Maykal Tsonov et al.
Perri Gish et al.
Bovine muscle satellite cells (MuSC) and fibro-adipogenic progenitor cells (FAP) are muscle resident stem cells that are responsible for postnatal muscle growth, intramuscular fat deposition, and extracellular matrix generation. These cells...
MDISCO: A High-Throughput Tissue-Clearing Protocol for Preservation of Endogenous Fluorescence in Whole Mouse Brains [0.03%]
MDISCO：一种用于保持整个小鼠大脑内源荧光的高通量组织清除协议
Madeline Martinez,Jake Thornberry,Akihiko Ozawa et al.
Madeline Martinez et al.
Organic solvent-based tissue clearing methods are widely used for whole-brain imaging but often compromise endogenous fluorescence. Existing protocols, such as iDISCO and fluorescence-preserving variants, have improved optical transparency ...
Cécile Fréreux,Philip H Howe
Cécile Fréreux
Cyclic GMP-AMP synthase (cGAS) is a key cytosolic double-stranded DNA sensor that activates innate immune responses. Upon binding double-stranded DNA, cGAS undergoes conformational activation and catalyzes the synthesis of the second messen...
A Simple Method for Estimating the Spatiotemporal Distribution of Phenoloxidase Proteins in Insect Tissues [0.03%]
昆虫组织中酚氧化酶蛋白时空分布的简易估计方法
Yusuke Nakatani,Yuji Matsuoka,Shinichi Morita et al.
Yusuke Nakatani et al.
Laccase2 (Lac2), a member of the phenoloxidase (PO) family, is an essential oxidase for melanin pigmentation in insects. The identification of the in vivo spatial distribution of Lac2 is crucial for understanding the molecular mechanisms un...
Preparing Adjuvanted Nanoliposomes for Applications Toward Recombinant Influenza Vaccine Development [0.03%]
制备佐剂纳米脂质体用于重组流感疫苗研发
Zachary R Sia,Wei-Chiao Huang,Matthew Willadsen et al.
Zachary R Sia et al.
Nanoparticle vaccines can provide advantages over traditional vaccine methodologies, including adjuvant delivery to enhance the effectiveness of recombinant antigens. Many approaches exist to formulate different vaccine nanoparticles, which...
Recovery and Expansion of Patient-Derived Glioblastoma Cells After Long-term Cryopreservation [0.03%]
长期冷冻保存后胶质母细胞瘤患者来源类器官的复苏及扩增
Wannawat Khotchawan,Chanchao Lorthongpanich,Pakpoom Kheolamai et al.
Wannawat Khotchawan et al.
Patient-derived glioblastoma (GBM) cells are valuable models for GBM research due to their rarity and the highly lethal nature of this cancer. Preserving these cells through long-term cryopreservation is therefore essential for advancing fu...
Hayao Ohno,Takanori Ida,Yuichi Iino
Hayao Ohno
The genome of the nematode Caenorhabditis elegans encodes at least 160 predicted peptide precursor genes that can generate over 300 bioactive peptides, the functions of most of which remain unknown. Phenotypes resulting from deletion or tra...