Manipulation of Gene Expression in Mouse Pancreas via Intraductal Delivery of Adeno-Associated Viral Vectors [0.03%]
通过胰管递送腺相关病毒载体在小鼠胰腺中操纵基因表达
Galina Soukhatcheva,Liam Stanley,Lei Dai et al.
Galina Soukhatcheva et al.
The rising global incidence of pancreatitis, pancreatic cancer, and diabetes has increased the need for efficient in vivo gene manipulation approaches to study the pancreas and develop new therapies. Although transgenic mouse models are wid...
Workflow for Crystallographic Fragment Screening by Crystal Soaking for Protein Targets: A Case Study on Thioredoxin Glutathione Reductase From Schistosoma mansoni [0.03%]
基于晶体浸泡的蛋白质晶相小分子筛选的工作流程及斯氏曼血吸虫硫氧还蛋白谷胱甘肽还原酶案例研究
Lauro R S Neto,Bogar O Montoya,José Brandão-Neto et al.
Lauro R S Neto et al.
Among the biophysical techniques used in fragment-based drug discovery (FBDD) campaigns, crystallography is the most sensitive, allowing for the identification of low-affinity ligands and the characterization of protein-ligand complexes at ...
TIE-UP-SIN: A Method for Enhanced Identification of Protein-Protein Interactions [0.03%]
一种增强的蛋白质-蛋白质相互作用识别方法(TIE-UP-SIN)
Maximilian Schedlowski,Stephan Michalik,Tilly Hoffmüller et al.
Maximilian Schedlowski et al.
Protein-protein interactions (PPIs) govern nearly all aspects of cellular physiology, yet identifying these interactions under native conditions remains challenging. Here, we present TIE-UP-SIN (targeted interactome experiment for unknown p...
Preparation and Assembly of the Axial Invasion Chamber for Live-Cell Invadopodia Imaging [0.03%]
活细胞侵袭足实时成像的轴向侵袭腔的制备和组装
Mark Garewal,Kenneth A Myers
Mark Garewal
Metastasis is initiated by cell invasion of the basement membrane, facilitating cell migration and colonization at a secondary tumor site. Cancer cells remodel the cytoskeleton to form ventral protrusions, termed invadopodia, that traffic a...
Protocol for Using CRISPR-Cas9 to Generate a Monocyte Cell Line Harboring a Single-Nucleotide Polymorphism [0.03%]
使用CRISPR-Cas9生成含有单核细胞系的单核苷酸多态性的协议
Amar Chandra Mahatha,Octavio Ramos-Espinosa,Dhemerson Souza de Lima et al.
Amar Chandra Mahatha et al.
We established a step-by-step approach for generating a single-nucleotide mutation in the promoter region of an immune regulatory gene in human monocyte THP-1 cells by employing a plasmid-based CRISPR-Cas9 system delivered via transfection ...
Efficient and Site-Specific Incorporation of 3-Nitro-Tyrosine Into Recombinant Proteins in Escherichia coli [0.03%]
在大肠杆菌中将3-硝基酪氨酸高效位点特异性地引入重组蛋白中
Sarah B McGee,Stanislau Stanisheuski,Ryan A Mehl et al.
Sarah B McGee et al.
3-nitro-tyrosine (nitroTyr) is one of numerous oxidative protein modifications implicated in diseases such as cardiovascular disease, cancer, and amyotrophic lateral sclerosis (ALS). Because of this, the ability to site-specifically encode ...
A Feeder Cell-Free System for Chimeric Antigen Receptor Gene Transduction Into Natural Killer Cells [0.03%]
一种用于T细胞受体基因转导至自然杀伤细胞的 feeder-free系统
Nobuhiro Kubo,Minori Baba,Yuko Suzuki et al.
Nobuhiro Kubo et al.
Anti-CD19 chimeric antigen receptor (CAR)-natural killer (NK) cells are expected to demonstrate anti-CD19 CAR-T-cell-like efficacy against relapsed and refractory B-cell malignancies and autoimmune diseases, with fewer adverse events and th...
From Design to Practice: A Comprehensive Tutorial for the Rapid Multiplex Engineering of Escherichia coli Using Antibiotic Resistance Markers [0.03%]
从设计到实践——使用抗生素抗性标记菌株快速多重改造大肠杆菌的全面教程
Shubhika Munot,Shuai Li,Jennifer N Hennigan et al.
Shubhika Munot et al.
Engineering of microbial cells, including E. coli, is essential in prototyping genetic designs used in numerous applications throughout synthetic biology. While many advanced genome editing tools, such as CRISPR-based tools, offer new capab...
Karthik Ramanadane,Elena F Lehmann,Cristina Manatschal
Karthik Ramanadane
Divalent metal ion transporters are conserved across all domains of life and play essential roles in diverse processes such as manganese acquisition during nutritional immunity in bacteria and iron homeostasis in higher eukaryotes [1-3]. Tr...
Quantitative Analysis of Splenic Natural Killer Cells of Mice Using Imaging Flow Cytometry [0.03%]
采用成像流式细胞术进行小鼠脾脏NK细胞的定量分析
Mohammad N Amin,Md Saqline Mostaq,Mohammad B Uddin et al.
Mohammad N Amin et al.
Natural killer (NK) cells are crucial innate immune effectors, mediating cytotoxicity against cancer and infected cells through receptors such as NKG2D. Reliable quantification of NK cell subsets is essential for evaluating NK cell-based im...