Parallelised Cloning, Mammalian Cell Expression, and Purification of Nanobodies Identified by Phage Display [0.03%]
噬菌体展示鉴定纳米抗体的平行克隆、哺乳动物细胞表达和纯化技术
Hannah A Campaigne,Kelly L Parker,Raymond J Owens et al.
Hannah A Campaigne et al.
Nanobodies are recombinant single-domain antibodies (VHHs) derived from the heavy chain-only subset of camelid immunoglobulins that can be reverse-engineered into bivalent antibodies by fusion to immunoglobulin Fc constant regions. Mammalia...
A Suspension-Trapping Protocol for Bottom-Up Proteomics Sample Preparation [0.03%]
一种用于自下而上蛋白质组样品制备的沉淀富集方法
Joseph Schrader,Dennis Province,Nicholas A DaSilva et al.
Joseph Schrader et al.
Bottom-up proteomics workflows encompass several key stages, including sample preparation, data acquisition, and data analysis. Of these, sample preparation is the initial and critical stage, as it significantly influences the depth, reprod...
Limited Proteolysis Mass Spectrometry to Identify Protein Structural Differences in Brain Tissue [0.03%]
有限位点酶解质谱法鉴定脑组织中的蛋白质结构差异
Haley E Tarbox,Stephen D Fried
Haley E Tarbox
Structural proteomics methods allow for the proteome-wide interrogation of protein structural differences between two different conditions. Limited proteolysis mass spectrometry (LiP-MS), as originally implemented by the Picotti lab, utiliz...
Assessing Mitochondrial Respiratory Complex-Associated Function From Previously Frozen Mouse Placental Tissue [0.03%]
从先前冷冻的小鼠胎盘组织评估与线粒体呼吸复合物相关的功能
Tina Podinic,Donald Xhuti,Cristina Monaco et al.
Tina Podinic et al.
The placenta is a metabolically active organ whose mitochondrial activity is tightly linked to fetal growth, oxygenation, and nutrient transport, mediating fetal susceptibility to environmental exposures. Accordingly, aberrant mitochondrial...
A Cell-Based Protocol to Assess Manganese Content and Relative Transport Activity of Manganese Transporters [0.03%]
一种基于细胞的协议，用于评估锰含量和锰转运蛋白的相对转运活性
Huiwei Zhong,Xurui Shen,Hanting Yang
Huiwei Zhong
Manganese (Mn) is an essential trace element whose intracellular homeostasis is tightly controlled by specialized membrane transporters. Dysregulation of Mn transport leads to pathological Mn accumulation and severe human disease; however, ...
Detecting Touch-Induced Calcium Dynamics With Live-Cell Imaging in Torenia Stigma [0.03%]
利用活细胞成像技术在紫金牛柱头上检测触摸诱导的钙动态变化
Xuan Zhou,Xiaofang Ma,Shuibo Yang et al.
Xuan Zhou et al.
Calcium ions serve as a universal secondary messenger, integrating diverse external signals, such as light, herbivory, and mechanical stimuli, within plant cells. However, the visualization and mechanistic dissection of calcium signaling sp...
Quantitative Assessment of Heat Shock-Induced Ferroptosis-Like Cell Death via Electrolyte Leakage in Arabidopsis thaliana Seedlings [0.03%]
拟南芥幼苗中热休克诱导的铁死亡-like细胞死亡的电解质渗漏定量评估
Leah Kago-Gachao,Clara Cappa Salas,Myriam Sanchis Campos et al.
Leah Kago-Gachao et al.
We present a protocol to allow continuous assessment of cell death in Arabidopsis thaliana (L.) seedlings by measuring the release of electrolytes from dying cells upon heat shock. The electrolyte leakage assay is a well-established method ...
Reconstitution of Active Plant H+-ATPase AHA2 in Giant Unilamellar Vesicles [0.03%]
植物H+-ATP酶AHA2在大型单层脂质体中的重构研究
Huriye D Uzun,Thomas Günther Pomorski
Huriye D Uzun
Membrane transporters mediate the selective movement of ions and molecules across biological membranes and are essential for cellular homeostasis. However, their functional characterization in living cells is often complicated by the comple...
Lipid Analysis in Live Caenorhabditis elegans Using Solution-State NMR Spectroscopy [0.03%]
溶液核磁共振活体分析秀丽杆线虫脂质分布动态
Florencia V Guastaferri,Carla B Delprato,Bruno Hernández Cravero et al.
Florencia V Guastaferri et al.
Unsaturated fatty acids (UFAs) play key roles in essential cellular functions such as membrane dynamics, metabolism, and animal development. Disruptions in UFA metabolism are linked to metabolic, cardiovascular, and neurodegenerative disord...
A Step-by-Step Protocol for the Isolation of Aloe vera-Derived Extracellular Vesicles via Manual and Shear-Force Homogenization [0.03%]
通过手动和剪切力均质化分离提取 aloevera衍生的细胞外囊泡的步步协议
Camila M Ceballos-Santa,Isabella Sanchez-Ortiz,Thomas R Gaborski et al.
Camila M Ceballos-Santa et al.
Aloe vera has long been used for its diverse pharmacological properties, motivating continued interest in isolating and preserving the bioactive molecules responsible for its therapeutic potential. More recently, Aloe vera-derived extracell...