A Versatile In Vitro Quantitative Assay for Macrophage Efferocytosis in Diverse Research Applications [0.03%]
一种用于不同研究应用中巨噬细胞凋亡细胞吞噬作用的多功能体外定量测定法
Xiaoge Xu,Tong Su,Qinuo Sun et al.
Xiaoge Xu et al.
Macrophage efferocytosis is a previously unrecognized key pathogenic event, engulfing apoptotic targets, preventing inflammation and necrosis, and maintaining immune homeostasis. The phagocytic function can be disrupted by harmful factors a...
Chemoenzymatic Labeling Method for Detection of O-GlcNAcylated α-Synuclein Proteins by Western Blot [0.03%]
用于Western印迹检测O-GlcNAc修饰α-突触核蛋白的化学酶法标记方法
Hongrui Meng,Zhuoya Ma,Yongzhen Miao et al.
Hongrui Meng et al.
α-Synuclein (α-syn) aggregation has emerged as a key pathogenetic feature in several neurodegenerative disorders. The α-syn protein has various conformational strains, each with unique structural features that influence their cytotoxicit...
TALENs and Related Technologies for Editing Nuclear and Organellar Genomes in a Model Plant, Arabidopsis thaliana [0.03%]
拟南芥核基因组和 organelle 基因组编辑的 TALEN 及相关技术
Chang Zhou,Shin-Ichi Arimura
Chang Zhou
Plant genome editing is a powerful approach for modifying plant DNA to investigate gene function and to engineer desirable traits. Several genome-editing technologies have been developed, among which CRISPR/Cas systems and transcription act...
Workflow for Fine-Tuning and Evaluating DNA Language Models for Specific Genomics Issues [0.03%]
针对特定基因组问题调整和评估DNA语言模型的工作流程
Kazuki Nakamae,Hidemasa Bono
Kazuki Nakamae
DNA language models, such as DNABERT-2, have recently enabled the accurate prediction of functional sequence elements across species. However, the practical, protocol-style steps needed to transform these resources into training datasets, f...
Assessment of Epithelial Barrier Integrity by TEER and FITC-Dextran Permeability Assays [0.03%]
上皮屏障完整性评价的_TEER_和_FITC-右旋糖酐_通透性试验法
Yagmur Keser,Steeve Boulant,Megan L Stanifer
Yagmur Keser
The integrity of epithelial barriers is essential for maintaining tissue homeostasis, particularly in the intestinal tract, where it separates the host from the complex luminal environment. Two complementary, standard methods for assessing ...
Accessible STORM Imaging: An Optimized Workflow for Conventional Widefield Epifluorescence/TIRF Setups [0.03%]
适用于常规宽场/总内反射荧光成像系统的STORM成像技术及优化方案
Jaime Fernández de Córdoba,Ana Oña,Gianluca D&#x;Agostino
Jaime Fernández de Córdoba
Stochastic optical reconstruction microscopy (STORM) is a single-molecule localization microscopy technique that enables visualization of cellular structures beyond the diffraction limit. This approach has revealed previously inaccessible u...
An Advanced Single-Cell RNA Sequencing (scRNA-seq) Protocol Utilizing Custom-Designed Multiplexing [0.03%]
一种利用定制多重设计的先进单细胞RNA测序（scRNA-seq）方案
Feng Gao,Xujie Liu,Fan Sun et al.
Feng Gao et al.
While cell hashing enhances single-cell RNA sequencing (scRNA-seq) efficiency and minimizes batch effects, commercial mouse hashtags often fail in FVB/N and several other strains due to antibody-epitope incompatibility. We describe a robust...
Generation of Functional Patient-Specific Thymus Organoids From Human Pluripotent Stem Cells (hPSCs) Using Air-Liquid Interface Culture [0.03%]
利用空气-液体界面培养技术从人多能干细胞生成个体特异胸腺类器官
Stephan A Ramos,Holger A Russ
Stephan A Ramos
The thymus is critical for the establishment of a functional and self-tolerant adaptive immune system, but it involutes with age, resulting in reduced naive T-cell output. Generation of a functional human thymus from human pluripotent stem ...
Yuval Milrad,Daniel Wegemann,Michael Hippler
Yuval Milrad
While traditional kinetic studies of the cytochrome b6f complex have frequently relied on measurements within the complex environment of intact leaves or whole-organism systems, such approaches can be limited by overlapping signals and phys...
Extraction and Isolation of Extracellular Vesicles From Piper betle Leaves Using the Apoplastic Fluid Washing and Size Exclusion Chromatography Method [0.03%]
使用胞间洗脱液洗涤和尺寸排阻色谱法从 Piper betle 叶片中提取和分离细胞外囊泡
Izy Sanddy,Wan Rahimatul Adawiyah,Der Jiun Ooi et al.
Izy Sanddy et al.
Plant-derived extracellular vesicles (PDEVs) have emerged as important mediators of intercellular communication and hold growing potential in therapeutic applications. However, standardized methods for their isolation, particularly from Pip...