Quantifying Single and Dual Channel Live Imaging Data: Kymograph Analysis of Organelle Motility in Neurons [0.03%]
定量分析单通道和双通道活细胞成像数据：神经元中 organelle 粒子运动的 kymograph 分析方法
Laura Digilio,Lloyd P McMahon,Alois Duston et al.
Laura Digilio et al.
Live imaging is commonly used to study dynamic processes in cells. Many labs carrying out live imaging in neurons use kymographs as a tool. Kymographs display time-dependent microscope data (time-lapsed images) in two-dimensional representa...
Measuring in vitro ATPase Activity with High Sensitivity Using Radiolabeled ATP [0.03%]
利用放射性标记ATP高灵敏度测定体外ATP酶活性
Sarina Veit,Thomas Günther Pomorski
Sarina Veit
ATPase assays are a common tool for the characterization of purified ATPases. Here, we describe a radioactive [γ-32P]-ATP-based approach, utilizing complex formation with molybdate for phase separation of the free phosphate from non-hydrol...
THRIFTY-A High-throughput Single Muscle Fiber Typing Method Based on Immunofluorescence Detection [0.03%]
THRIFTY-一种基于免疫荧光检测的高通量单肌纤维分型方法
Sebastian Edman,Oscar Horwath,William Apró
Sebastian Edman
Skeletal muscle consists of a mixture of fiber types with different functional and metabolic characteristics. The relative composition of these muscle fiber types has implications for muscle performance, whole-body metabolism, and health. H...
Shao-Chin Wu,Chi-Hung Lin
Shao-Chin Wu
The adipose tissue is a central metabolic organ that regulates whole-body energy homeostasis. The abnormal expansion of adipose tissue leads to the progression of obesity. The adipose tissue microenvironment is affected by pathological hype...
Mitochondrial Replication Assay (MIRA) for Efficient in situ Quantification of Nascent mtDNA and Protein Interactions with Nascent mtDNA (mitoSIRF) [0.03%]
线粒体复制分析(MIRA):一种有效的原位定量新生mtDNA和蛋白质与新生mtDNA相互作用的方法(线粒体SIRF法)
Macy Lozen,Yue Chen,Rebecca A Boisvert et al.
Macy Lozen et al.
Mitochondria play decisive roles in bioenergetics and intracellular communication. These organelles contain a circular mitochondrial DNA (mtDNA) genome that is duplicated within one to two hours by a mitochondrial replisome, independently f...
In vivo Drug Screening to Identify Anti-metastatic Drugs in Twist1a-ERT2 Transgenic Zebrafish [0.03%]
体内药物筛选识别Twist1a-ERT2转基因斑马鱼抗转移药物
Joji Nakayama,Hideki Makinoshima,Zhiyuan Gong
Joji Nakayama
Here, we present an in vivo drug screening protocol using a zebrafish model of metastasis for the identification of anti-metastatic drugs. A tamoxifen-controllable Twist1a-ERT2 transgenic zebrafish line was established to serve as a platfor...
Implementation of a Drug Screening Platform to Target Gch1 Expression in Injured Mouse Dorsal Root Ganglion Neurons [0.03%]
一种针对损伤的小鼠背根神经节神经元Gch1表达的药物筛选平台的建立及应用
Shane J F Cronin,Lance S Davidow,Anthony C Arvanites et al.
Shane J F Cronin et al.
Management of neuropathic pain is notoriously difficult; current analgesics, including anti-inflammatory- and opioid-based medications, are generally ineffective and can pose serious side effects. There is a need to uncover non-addictive an...
Induction of Skeletal Muscle Injury by Intramuscular Injection of Cardiotoxin in Mouse [0.03%]
内毒素诱导的小鼠肌肉损伤模型的建立及淫羊藿茋的保护作用
Xin Fu,Sheng Li,Minzhi Jia et al.
Xin Fu et al.
Skeletal muscle is the most abundant tissue in the human body and has a tremendous capability to regenerate in response to muscle injuries and diseases. Induction of acute muscle injury is a common method to study muscle regeneration in viv...
E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT [0.03%]
使用Bruker Skyscan 1172微CT进行E15.5期鼠胚的成像
Elena Astanina,Sara Petrillo,Tullio Genova et al.
Elena Astanina et al.
X-ray computed microtomography (µCT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphol...
Modified Pseudo-Schiff Propidium Iodide for Staining the Shoot Apical Meristem in Arabidopsis [0.03%]
改良的伪席夫亚甲基蓝染色法在拟南芥芽尖 meristem 中的应用
Ruiqi Li,Qi Li,Ligeng Ma
Ruiqi Li
Visualization of cell structure with fluorescent dye for characterizing cell size, shape, and arrangement is a common method to study tissue morphology and morphogenesis. In order to observe shoot apical meristem (SAM) in Arabidopsis thalia...