Deaminase-Assisted Sequencing for the Identification of 5-glyceryl-methylcytosine [0.03%]
脱氨酶辅助测序技术表征DNA中5-甘油甲基胞嘧啶修饰
Bao-Dan He,Fan-Chen Wang,Jian-Huang Xue
Bao-Dan He
DNA epigenetic modifications play crucial roles in regulating gene expression and cellular function across diverse organisms. Among them, 5-glyceryl-methylcytosine (5gmC), a unique DNA modification first discovered in Chlamydomonas reinhard...
Optimized Mechanical Isolation of Mitochondria From Saccharomyces cerevisiae Preserving Atg32 for Quantitative Analysis [0.03%]
一种优化的机械法用于从酿酒酵母中分离线粒体以保留Atg32进行量化分析
Ariann E Mendoza-Martínez,J Ernesto Bravo-Arévalo,Ulrik Pedroza-Dávila et al.
Ariann E Mendoza-Martínez et al.
Mitophagy is a highly conserved process among eukaryotic cells, playing a primordial role in mitochondrial quality control and overall cellular homeostasis. In Saccharomyces cerevisiae, Atg32 is the only identified mitophagy receptor locali...
Step-by-Step Protocol for In Situ Profiling of RNA Subcellular Localization Using TATA-seq [0.03%]
使用TATA-seq进行RNA亚细胞定位原位分析的协议
Xiao Jiang,Chu Xu,Lulu Hu
Xiao Jiang
Membrane-less organelles play essential roles in both physiological and pathological processes by compartmentalizing biomolecules through phase separation to form dynamic hubs. These hubs enable rapid responses to cellular stress and help m...
Nick Lang,Catherine G Chu,Andrew D Stephens
Nick Lang
Time-lapse into immunofluorescence (TL into IF) imaging combines the wealth of information acquired during live-cell imaging with ease of access for static immunofluorescence markers. In the field of mechanobiology, connecting live and stat...
In Vitro Model of Cytokine-Induced Inflammatory 3T3-L1 Adipocytes Mimicking Obesity [0.03%]
体外细胞因子诱导的小鼠3T3-L1脂肪细胞炎症模型构建及评价肥胖炎症表型的作用
Lucille Cartier,Rémi Fournet,Manon De Boni et al.
Lucille Cartier et al.
Obesity is a risk factor for many diseases. The 3T3-L1 cell line is often used to obtain mature adipocytes, but these lack the inflammatory phenotype observed in obesity. Using a cocktail of cytokines that mimics the secretome of macrophage...
Introducing Exogenous DNA Vectors Directly into Trypoxylus dichotomus Larvae Via In Vivo Electroporation [0.03%]
通过活体电穿孔技术将外源DNA直接导入白星银线虫幼虫体内
Shinichi Morita,Teruyuki Niimi
Shinichi Morita
In the Japanese rhinoceros beetle Trypoxylus dichotomus, gene function studies have relied mainly on systemic larval RNA interference (RNAi), as gain-of-function techniques remain underdeveloped and germline transgenesis is impractical give...
Visual Nanoprobe-Enhanced Loop-Mediated Isothermal Amplification Protocol for Rapid Detection of Infectious Laryngotracheitis Virus from Avian Respiratory Swabs [0.03%]
用于从鸟类呼吸道拭子中快速检测传染性喉气管炎病毒的视觉纳米探针增强环介导等温扩增法
Pablo Cea-Callejo,Claudia Trenado,Ana Doménech et al.
Pablo Cea-Callejo et al.
A prompt and accurate diagnosis of respiratory viral diseases in intensive poultry production is essential to safeguard animal health and ensure the economic sustainability of farms. Currently, much effort is being devoted to preventing the...
Lauren A Higa,Taren Bouwman,Zhi-Yan Du
Lauren A Higa
Protoplast systems are widely used in plant research as versatile platforms for studying cellular processes and validating gene editing tools. In maize, they are particularly valuable because stable transformation in immature embryos is slo...
Simple and Rapid Model to Generate Differentiated Endometrial Floating Organoids [0.03%]
一种简单快速生成分化子宫内膜浮游类器官的模型
Adriana Bajetto,Alessandra Pattarozzi,Alessandro Corsaro et al.
Adriana Bajetto et al.
Nowadays, the use of 3D cultures (organoids) is considered a valuable experimental tool to model physiological and pathological conditions of organs and tissues. Organoids, retaining cellular heterogeneity with the presence of stem, progeni...
The Generation of Tissue-Specific ECM Hydrogels From Melanoma and Associated Organs to Study Cancer Biology [0.03%]
用于癌症生物学研究的黑色素瘤及相应器官组织特异性ECM水凝胶的制备
Yuval Mogilevsky,Chen Sharon-Yagol,Bar Manobla et al.
Yuval Mogilevsky et al.
The extracellular matrix (ECM) critically shapes melanoma progression and therapeutic response, yet commonly used matrices such as Matrigel fail to capture tissue- and disease-specific ECM properties. This protocol provides a streamlined an...