Digital Magnetic Sorting for Fractionating Cell Populations with Variable Antigen Expression in Cell Therapy Process Development [0.03%]
基于细胞治疗工艺开发的可变抗原表达细胞群体的数字磁性分选
Savannah Bshara-Corson,Andrew Burwell,Timothy Tiemann et al.
Savannah Bshara-Corson et al.
Cellular therapies exhibit immense potential in treating complex diseases with sustained responses. The manufacture of cell therapies involves the purification and engineering of specific cells from a donor or patient to achieve a therapeut...
Solution NMR Backbone Assignment of the C-Terminal Region of Human Dynein Light Intermediate Chain 2 (LIC2-C) Unveils Structural Resemblance with Its Homologue LIC1-C [0.03%]
人源动力蛋白中间轻链2的C端区域（LIC2-C）溶液结构NMR主链共振 ASSIGNMENT揭示其与同系物LIC1-C具有结构相似性
Morkos A Henen,Natasia Paukovich,Rytis Prekeris et al.
Morkos A Henen et al.
Dynein, a homodimeric protein complex, plays a pivotal role in retrograde transportation along microtubules within cells. It consists of various subunits, among which the light intermediate chain (LIC) performs diverse functions, including ...
A Simulation Independent Analysis of Single- and Multi-Component cw ESR Spectra [0.03%]
一种独立于模拟的连续波ESR光谱单成分和多成分分析方法
Aritro Sinha Roy,Boris Dzikovski,Dependu Dolui et al.
Aritro Sinha Roy et al.
The accurate analysis of continuous-wave electron spin resonance (cw ESR) spectra of biological or organic free-radicals and paramagnetic metal complexes is key to understanding their structure-function relationships and electrochemical pro...
Aritro Sinha Roy,Madhur Srivastava
Aritro Sinha Roy
The objective of spectral analysis is to resolve and extract relevant features from experimental data in an optimal fashion. In continuous-wave (cw) electron spin resonance (ESR) spectroscopy, both g values of a paramagnetic center and hype...
Slow methyl axes motions in perdeuterated villin headpiece subdomain probed by cross-correlated NMR relaxation measurements [0.03%]
利用交叉相关NMR弛豫测量探测去uterated villin头部区段的缓慢甲基轴向运动
Liliya Vugmeyster,Parker J Nichols,Dmitry Ostrovsky et al.
Liliya Vugmeyster et al.
Protein methyl groups can participate in multiple motional modes on different time scales. Sub-nanosecond to nano-second time scale motions of methyl axes are particularly challenging to detect for small proteins in solutions. In this work ...
Enrichment and Detection of Antigen-Binding B Cells for Mass Cytometry [0.03%]
用于质谱细胞分析的抗原结合B细胞的富集和检测
Zachary C Stensland,Mia J Smith
Zachary C Stensland
Over the years, various techniques have been utilized to study the function and phenotype of antigen-binding B cells in the primary repertoire following immunization, infection, and development of autoimmunity. Due to the low frequency of a...
Tyler M Ozvat,Spencer H Johnson,Anthony K Rappé et al.
Tyler M Ozvat et al.
Studying the correlation between temperature-driven molecular structure and nuclear spin dynamics is essential to understanding fundamental design principles for thermometric nuclear magnetic resonance spin-based probes. Herein, we study th...
Efficient Stereospecific Hβ2/3 NMR Assignment Strategy for Mid-Size Proteins [0.03%]
中等大小蛋白质Hβ2/3高效立体专一性NMR谱鉴定策略
Alexandra Born,Morkos A Henen,Parker Nichols et al.
Alexandra Born et al.
We present a strategy for stereospecific NMR assignment of Hβ2 and Hβ3 protons in mid-size proteins (~150 residues). For such proteins, resonance overlap in standard experiments is severe, thereby preventing unambiguous assignment of a la...
Structural changes of Zn(II)bleomycin complexes when bound to DNA hairpins containing the 5'-GT-3' and 5'-GC-3' binding sites studied through NMR spectroscopy [0.03%]
结合位点的NMR研究锌菌灰酶与含有5'-GT-3'和5'-GC-3'的发夹DNA复合物结构变化
Shelby E Follett,Sally A Murray,Azure D Ingersoll et al.
Shelby E Follett et al.
Bleomycins are antitumor antibiotics that can chelate a metal center and cause site-specific DNA cleavage at 5'-Gpyrimidine-3' regions of DNA. These antibiotics are successful in the treatment of various cancers, but are known to cause pulm...