Ampicillin treatment in persister cell studies may cause non-physiological artifacts [0.03%]
青霉素类药物在持久态菌细胞研究中可能导致非生理学伪像
Michel Fasnacht,Hena Comic,Isabella Moll
Michel Fasnacht
Persister cells are a clinically relevant sub-population of an isogenic bacterial culture that is tolerant to bactericidal antibiotics. With the aim to investigate the ribosomal protein content of persister cells, we employed the bacterioly...
Unveiling the molecular architecture of the mitochondrial respiratory chain of Acanthamoeba castellanii [0.03%]
解密卡氏棘阿米巴线粒体呼吸链分子结构
Christian Q Scheckhuber,Sutherland K Maciver,Alvaro de Obeso Fernandez Del Valle
Christian Q Scheckhuber
Acanthamoeba castellanii is a ubiquitous free-living amoeba that can cause severe infections in humans. Unlike most other organisms, A. castellanii possesses a "complete" mitochondrial respiratory chain, meaning it con-tains several additio...
Paving the way for new antimicrobial peptides through molecular de-extinction [0.03%]
通过分子灭绝反演为新的抗菌肽铺平道路
Karen O Osiro,Abel Gil-Ley,Fabiano C Fernandes et al.
Karen O Osiro et al.
Molecular de-extinction has emerged as a novel strategy for studying biological molecules throughout evolutionary history. Among the myriad possibilities offered by ancient genomes and proteomes, antimicrobial peptides (AMPs) stand out as p...
Integrative Omics reveals changes in the cellular landscape of peroxisome-deficient pex3 yeast cells [0.03%]
综合组学揭示了过氧化物酶体缺乏的pex3酵母细胞的细胞景观变化
Tjasa Kosir,Hirak Das,Marc Pilegaard Pedersen et al.
Tjasa Kosir et al.
Peroxisomes are organelles that are crucial for cellular metabolism, but they also play important roles in non-metabolic processes such as signalling, stress response or antiviral defense. To uncover the consequences of peroxisome deficienc...
It takes four to tango: the cooperative adventure of scientific publishing [0.03%]
探秘科学出版的合作冒险：四人共舞的故事
Didac Carmona-Gutierrez,Katharina Kainz,Frank Madeo
Didac Carmona-Gutierrez
The publication and scientific implementation of scholarly articles is a collaborative effort that unites readers, authors, editors, and referees. A scientific journal thereby serves as a vital platform, enabling these interactions and fost...
Microwave-assisted preparation of yeast cells for ultrastructural analysis by electron microscopy [0.03%]
微波辅助法制备用于电子显微镜分析的酵母细胞
Moritz Mayer,Christina Schug,Stefan Geimer et al.
Moritz Mayer et al.
Budding yeast Saccharomyces cerevisiae is widely used as a model organism to study the biogenesis and architecture of organellar membranes, which can be visualized by transmission electron microscopy (TEM). Preparation of yeast cells for TE...
Efflux pumps: gatekeepers of antibiotic resistance in Staphylococcus aureus biofilms [0.03%]
外排泵：金黄色葡萄球菌生物被膜抗菌素耐药性的守门者
Shweta Sinha,Shifu Aggarwal,Durg Vijai Singh
Shweta Sinha
Staphylococcus aureus, a versatile human pathogen, poses a significant challenge in healthcare settings due to its ability to develop antibiotic resistance and form robust biofilms. Understanding the intricate mechanisms underlying the anti...
RidA proteins contribute to fitness of S. enterica and E. coli by reducing 2AA stress and moderating flux to isoleucine biosynthesis [0.03%]
RidA蛋白通过降低氨基酸压力并调节异亮氨酸生物合成通量来增强沙门氏菌和大肠杆菌的适应性
Ronnie L Fulton,Bryce R Sawyer,Diana M Downs
Ronnie L Fulton
Defining the physiological role of a gene product relies on interpreting phenotypes caused by the lack, or alteration, of the respective gene product. Mutations in critical genes often lead to easily recognized phenotypes that can include c...
A complex remodeling of cellular homeostasis distinguishes RSV/SARS-CoV-2 co-infected A549-hACE2 expressing cell lines [0.03%]
复杂的细胞稳态重塑区分RSV/SARS-CoV-2共感染的A549-hACE2表达细胞系
Claudia Vanetti,Irma Saulle,Valentina Artusa et al.
Claudia Vanetti et al.
Concurrent infections with two or more pathogens with analogous tropism, such as RSV and SARS-CoV-2, may antagonize or facilitate each other, modulating disease outcome. Clinically, discrepancies in the severity of symptoms have been report...
Fecal gelatinase does not predict mortality in patients with alcohol-associated hepatitis [0.03%]
粪胶原酶不能预测酒精性肝炎患者的死亡率
Yongqiang Yang,Phillipp Hartmann,Bernd Schnabl
Yongqiang Yang
Alcohol-associated liver disease is highly prevalent worldwide, with alcohol-associated hepatitis as a severe form characterized by substantial morbidity, mortality, and economic burden. Gut bacterial dysbiosis has been linked to progressio...