Identification of eyeworm (Oxyspirura petrowi) and caecal worm (Aulonocephalus pennula) infection levels in Northern bobwhite quail (Colinus virginianus) of the Rolling Plains, TX using a mobile research laboratory: Implications for regional surveillance [0.03%]
基于移动实验室技术对美国德克萨斯州诺思伯劳克赤胸鹌鹑眼线虫和盲囊尾线虫的感染现状调查及对该地区进行区域性监测的意义
Kendall R Blanchard,Aravindan Kalyanasundaram,Cassandra Henry et al.
Kendall R Blanchard et al.
Over the last few decades, there has been a decline in Northern bobwhite quail (Colinus virginianus) throughout their native range. While there are various factors that may be influencing this decline, it is suggested that parasites should ...
For what factors should we normalize urinary extracellular mRNA biomarkers? [0.03%]
尿液细胞外mRNA生物标志物的标准化需考虑哪些因素？
Pradeep Moon Gunasekaran,James Matthew Luther,James Brian Byrd
Pradeep Moon Gunasekaran
mRNA is a critical biomolecule involved in the manifestation of the genetic code into functional protein molecules. Its critical role in the central dogma has made it a key target in many studies to determine biomarkers and drug targets for...
Adam L Millington,Jessica A Houskeeper,John F Quackenbush et al.
Adam L Millington et al.
The kinetic requirements of quantitative PCR were experimentally dissected into the stages of DNA denaturation, primer annealing, and polymerase extension. The temperature/time conditions for 2 stages were kept optimal, while the other was ...
Corrigendum to "Considerations and quality controls when analyzing cell-free tumor DNA" [Biomol. Detect. Quantif. 17 (2019) 100078] [0.03%]
关于“分析游离肿瘤DNA时的注意事项和质量控制”的勘误(生物分子检测与定量第17卷(2019)100078号论文)
Gustav Johansson,Daniel Andersson,Stefan Filges et al.
Gustav Johansson et al.
[This corrects the article DOI: 10.1016/j.bdq.2018.12.003.].
Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing-Enabling robust and non-invasive biomarker research [0.03%]
用于RNA测序的尿液外泌体富集方法比较——促进稳健且无创的生物标志物研究
Veronika Mussack,Georg Wittmann,Michael W Pfaffl
Veronika Mussack
Small extracellular vesicles (EVs) are 50-200 nm sized mediators in intercellular communication that reflect both physiological and pathophysiological changes of their parental cells. Thus, EVs hold great potential for biomarker detection. ...
Joel Tellinghuisen,Andrej-Nikolai Spiess
Joel Tellinghuisen
The standard approach for quantitative estimation of genetic materials with qPCR is calibration with known concentrations for the target substance, in which estimates of the quantification cycle (Cq ) are fitted to a straight-line function ...
Corrigendum to "Development of event-specific qPCR detection methods for genetically modified alfalfa events J101, J163 and KK179" [Biomol. Detect. Quantif. 17 (March) (2019) 100076] [0.03%]
关于“转基因紫花苜蓿事件J101、J163和KK179的事件特异性qPCR检测方法的发展”[Biomol. Detect. Quantif. 17 (March) (2019) 100076]的更正
Patrick Guertler,Lutz Grohmann,Heike Naumann et al.
Patrick Guertler et al.
[This corrects the article DOI: 10.1016/j.bdq.2018.12.001.].
Establishing essential quality criteria for the validation of circular RNAs as biomarkers [0.03%]
建立环形RNA作为生物标志物验证的基本质量标准riteria
Christina Pfafenrot,Christian Preußer
Christina Pfafenrot
Non-coding RNAs were established in the last decade as a new valuable biomarker class for human diseases. Specifically, circular RNAs (circRNAs) were only recently discovered as a new large group of non-coding RNAs that, due to their circul...
Development of event-specific qPCR detection methods for genetically modified alfalfa events J101, J163 and KK179 [0.03%]
抗草甘膦转基因苜蓿J101、J163和KK179特异事件的实时荧光定量PCR检测方法研究
Patrick Guertler,Lutz Grohmann,Heike Naumann et al.
Patrick Guertler et al.
Genetically modified alfalfa is authorized for cultivation in several countries since 2005. On the other hand, cultivation in or export to the European Union is not allowed and thus neither certified reference material nor official event-sp...
Michael W Pfaffl
Michael W Pfaffl