Re-evaluation of the impact of BUD21 deletion on xylose utilization by Saccharomyces cerevisiae [0.03%]
对BUD21基因敲除对酿酒酵母木糖利用影响的再评估
Venkatachalam Narayanan,Anders G Sandström,Marie F Gorwa-Grauslund
Venkatachalam Narayanan
Various rational metabolic engineering and random approaches have been applied to introduce and improve xylose utilization and ethanol productivity by Saccharomyces cerevisiae. Among them, the BUD21 gene was identified as an interesting can...
Adaptive laboratory evolution of Bacillus subtilis to overcome toxicity of lignocellulosic hydrolysate derived from Distiller's dried grains with solubles (DDGS) [0.03%]
芽孢杆菌适应性实验室进化以克服从干酒糟与溶剂残余物(DGDS)中获得的木质纤维素水解液毒性的研究
Jasper L S P Driessen,Josefin Johnsen,Ivan Pogrebnyakov et al.
Jasper L S P Driessen et al.
Microbial tolerance to toxic compounds formed during biomass pretreatment is a significant challenge to produce bio-based products from lignocellulose cost effectively. Rational engineering can be problematic due to insufficient prerequisit...
Exploring linker's sequence diversity to fuse carotene cyclase and hydroxylase for zeaxanthin biosynthesis [0.03%]
探索连接肽的序列多样性以融合类胡萝卜素环化酶和羟基化酶用于紫黄素生物合成
Aurélie Bouin,Congqiang Zhang,Nic D Lindley et al.
Aurélie Bouin et al.
Fusion of catalytic domains can accelerate cascade reactions by bringing enzymes in close proximity. However, the design of a protein fusion and the choice of a linker are often challenging and lack of guidance. To determine the impact of l...
Engineered production of isoprene from the model green microalga Chlamydomonas reinhardtii [0.03%]
模式绿藻小立藻中异戊二烯的工程化生产
Razan Z Yahya,Gordon B Wellman,Sebastian Overmans et al.
Razan Z Yahya et al.
Isoprene is a clear, colorless, volatile 5-carbon hydrocarbon that is one monomer of all cellular isoprenoids and a platform chemical with multiple applications in industry. Many plants have evolved isoprene synthases (IspSs) with the capac...
Metabolic engineering of low-pH-tolerant non-model yeast, Issatchenkia orientalis, for production of citramalate [0.03%]
利用低PH值耐受非模式酵母Issatchenkia orientalis代谢工程生产柠檬羟酸
Zong-Yen Wu,Wan Sun,Yihui Shen et al.
Zong-Yen Wu et al.
Methyl methacrylate (MMA) is an important petrochemical with many applications. However, its manufacture has a large environmental footprint. Combined biological and chemical synthesis (semisynthesis) may be a promising alternative to reduc...
Novel insights into construct toxicity, strain optimization, and primary sequence design for producing recombinant silk fibroin and elastin-like peptide in E. coli [0.03%]
大肠杆菌中重组丝素和弹性肽产生的构建毒性、菌株优化及一级序列设计的新见解
Alexander Connor,Caleb Wigham,Yang Bai et al.
Alexander Connor et al.
Spider silk proteins (spidroins) are a remarkable class of biomaterials that exhibit a unique combination of high-value attributes and can be processed into numerous morphologies for targeted applications in diverse fields. Recombinant prod...
Cell-free metabolic engineering enables selective biotransformation of fatty acids to value-added chemicals [0.03%]
无细胞代谢工程实现脂肪酸向高附加值化学品的选择性生物转化
Yushi Liu,Wan-Qiu Liu,Shuhui Huang et al.
Yushi Liu et al.
Fatty acid-derived products such as alkanes, fatty aldehydes, and fatty alcohols have many applications in the chemical industry. These products are predominately produced from fossil resources, but their production processes are often not ...
Improved protein glycosylation enabled heterologous biosynthesis of monoterpenoid indole alkaloids and their unnatural derivatives in yeast [0.03%]
改善的蛋白质糖基化使单萜吲哚生物碱及其非天然衍生物在酵母中的异源生物合成成为可能
Mohammadamin Shahsavarani,Joseph Christian Utomo,Rahul Kumar et al.
Mohammadamin Shahsavarani et al.
With over 3000 reported structures, monoterpenoid indole alkaloids (MIAs) constitute one of the largest alkaloid groups in nature, including the clinically important anticancer drug vinblastine and its semi-synthetic derivatives from Cathar...
Characterization and engineering of the xylose-inducible xylP promoter for use in mold fungal species [0.03%]
木糖诱导的xylP启动子在 molds 真菌中的特征和应用研究
Annie Yap,Irene Glarcher,Matthias Misslinger et al.
Annie Yap et al.
Conditional promoters allowing both induction and silencing of gene expression are indispensable for basic and applied research. The xylP promoter (pxylP) from Penicillium chrysogenum was demonstrated to function in various mold species inc...
Genome-scale reconstruction and metabolic modelling of the fast-growing thermophile Geobacillus sp. LC300 [0.03%]
快速生长的嗜热菌地芽孢杆菌LC300的基因组规模重建及其代谢建模
Emil Ljungqvist,Martin Gustavsson
Emil Ljungqvist
Thermophilic microorganisms show high potential for use as biorefinery cell factories. Their high growth temperatures provide fast conversion rates, lower risk of contaminations, and facilitated purification of volatile products. To date, o...