Bottom-up parameterization of enzyme rate constants: Reconciling inconsistent data [0.03%]
基于底物浓度谱反求酶反应动力学参数：解决数据冲突问题
Daniel C Zielinski,Marta R A Matos,James E de Bree et al.
Daniel C Zielinski et al.
Kinetic models of metabolism are promising platforms for studying complex metabolic systems and designing production strains. Given the availability of enzyme kinetic data from historical experiments and machine learning estimation tools, a...
2-Stage microfermentations [0.03%]
两阶段微型发酵试验
Shuai Li,Zhixia Ye,Eirik A Moreb et al.
Shuai Li et al.
Cell based factories can be engineered to produce a wide variety of products. Advances in DNA synthesis and genome editing have greatly simplified the design and construction of these factories. It has never been easier to generate hundreds...
Building blocks needed for mechanistic modeling of bioprocesses: A critical review based on protein production by CHO cells [0.03%]
基于CHO细胞产蛋白的机理建模所需构建模块的重要性的批判性综述
Yusmel González-Hernández,Patrick Perré
Yusmel González-Hernández
This paper reviews the key building blocks needed to develop a mechanistic model for use as an operational production tool. The Chinese Hamster Ovary (CHO) cell, one of the most widely used hosts for antibody production in the pharmaceutica...
A new concept of biocatalytic synthesis of acrylic monomers for obtaining water-soluble acrylic heteropolymers [0.03%]
用于获得水溶性丙烯酸杂聚合物的丙烯酸单体生物催化合成的新概念
Konstantin V Lavrov,Anna O Shemyakina,Elena G Grechishnikova et al.
Konstantin V Lavrov et al.
Rhodococcus strains were designed as model biocatalysts (BCs) for the production of acrylic acid and mixtures of acrylic monomers consisting of acrylamide, acrylic acid, and N-alkylacrylamide (N-isopropylacrylamide). To obtain BC strains, w...
Rewiring Saccharomyces cerevisiae metabolism for optimised Taxol® precursors production [0.03%]
优化诺沃宾前体产生的酿酒酵母代谢重组
Behnaz Nowrouzi,Pablo Torres-Montero,Eduard J Kerkhoven et al.
Behnaz Nowrouzi et al.
Saccharomyces cerevisiae has been conveniently used to produce Taxol® anticancer drug early precursors. However, the harmful impact of oxidative stress by the first cytochrome P450-reductase enzymes (CYP725A4-POR) of Taxol® pathway has ha...
Ayushi Agrawal,Zhiliang Yang,Mark Blenner
Ayushi Agrawal
Geraniol is a monoterpene with wide applications in the food, cosmetics, and pharmaceutical industries. Microbial production has largely used model organisms lacking favorable properties for monoterpene production. In this work, we produced...
Have you tried turning it off and on again? Oscillating selection to enhance fitness-landscape traversal in adaptive laboratory evolution experiments [0.03%]
你试过重启了吗？通过选择振荡增强适应性实验室进化实验中的 fitness-landscape 穿越能力
Alexander C Carpenter,Adam M Feist,Fergus S M Harrison et al.
Alexander C Carpenter et al.
Adaptive Laboratory Evolution (ALE) is a powerful tool for engineering and understanding microbial physiology. ALE relies on the selection and enrichment of mutations that enable survival or faster growth under a selective condition imposed...
Engineered ketocarotenoid biosynthesis in the polyextremophilic red microalga Cyanidioschyzon merolae 10D [0.03%]
在极端耐受性红微藻Cyanidioschyzon merolae 10D中工程化构建酮类胡萝卜素合成途径
Mark Seger,Fakhriyya Mammadova,Melany Villegas-Valencia et al.
Mark Seger et al.
The polyextremophilic Cyanidiophyceae are eukaryotic red microalgae with promising biotechnological properties arising from their low pH and elevated temperature requirements which can minimize culture contamination at scale. Cyanidioschyzo...
Engineering of increased L-Threonine production in bacteria by combinatorial cloning and machine learning [0.03%]
基于组合型克隆和机器学习的工程菌合成L-丝氨酸和D-3磷酸甘油酸技術
Paul Hanke,Bruce Parrello,Olga Vasieva et al.
Paul Hanke et al.
The goal of this study is to develop a general strategy for bacterial engineering using an integrated synthetic biology and machine learning (ML) approach. This strategy was developed in the context of increasing L-threonine production in E...
Optimization of a hybrid bacterial/ Arabidopsis thaliana fatty acid synthase system II in Saccharomyces cerevisiae [0.03%]
优化大肠杆菌和拟南芥脂肪酸合成酶系统II在酿酒酵母中的杂合表达体系
Tatiana A Pozdniakova,João P Cruz,Paulo César Silva et al.
Tatiana A Pozdniakova et al.
Fatty acids are produced by eukaryotes like baker's yeast Saccharomyces cerevisiae mainly using a large multifunctional type I fatty acid synthase (FASI) where seven catalytic steps and a carrier domain are shared between one or two protein...