A comparative analysis of NADPH supply strategies in Saccharomyces cerevisiae: Production of d-xylitol from d-xylose as a case study [0.03%]
以D-木糖制备D-木醇为例分析酿酒酵母中烟酰胺腺嘌呤二核苷酸磷酸供应策略的比较研究
Priti Regmi,Melanie Knesebeck,Eckhard Boles et al.
Priti Regmi et al.
Enhancing the supply of the redox cofactor NADPH in metabolically engineered cells is a critical target for optimizing the synthesis of many product classes, such as fatty acids or terpenoids. In S. cerevisiae, several successful approaches...
R P van Rosmalen,S Moreno-Paz,Z E Duman-Özdamar et al.
R P van Rosmalen et al.
Genome-scale metabolic models of microbial metabolism have extensively been used to guide the design of microbial cell factories, still, many of the available strain design algorithms often fail to produce a reduced list of targets for impr...
Expression and characterization of monofunctional alcohol dehydrogenase enzymes in Clostridium thermocellum [0.03%]
Clostridium thermocellum中单功能型酒精脱氢酶的表达与鉴定
Daniela Prates Chiarelli,Bishal Dev Sharma,Shuen Hon et al.
Daniela Prates Chiarelli et al.
Clostridium thermocellum is a thermophilic anaerobic bacterium that could be used for cellulosic biofuel production due to its strong native ability to consume cellulose, however its ethanol production ability needs to be improved to enable...
Metabolic bottlenecks of Pseudomonas taiwanensis VLB120 during growth on d-xylose via the Weimberg pathway [0.03%]
Pseudomonas taiwanensis VLB120 在通过 Weimberg途径利用 d-木糖生长过程中的代谢限速步骤
Philipp Nerke,Jonas Korb,Frederick Haala et al.
Philipp Nerke et al.
The microbial production of value-added chemicals from renewable feedstocks is an important step towards a sustainable, bio-based economy. Therefore, microbes need to efficiently utilize lignocellulosic biomass and its dominant constituents...
Sustainable biosynthesis of squalene from waste cooking oil by the yeast Yarrowia lipolytica [0.03%]
利用酵母Yarrowia lipolytica从废餐饮油中可持续合成鲨烯
Shuhui Wang,Xu Sun,Yuqing Han et al.
Shuhui Wang et al.
Squalene is a highly sought-after triterpene compound in growing demand, and its production offers a promising avenue for circular economy practices. In this study, we applied metabolic engineering principles to enhance squalene production ...
13C-metabolic flux analysis reveals metabolic rewiring in HL-60 neutrophil-like cells through differentiation and immune stimulation [0.03%]
基于同位素13C代谢流分析白血病细胞HL-60通过分化和免疫刺激诱导的代谢重编程
Takeo Taniguchi,Nobuyuki Okahashi,Fumio Matsuda
Takeo Taniguchi
Neutrophils are innate immune cells and the first line of defense for the maintenance of homeostasis. However, our knowledge of the metabolic rewiring associated with their differentiation and immune stimulation is limited. Here, quantitati...
Engineering a carbon source-responsive promoter for improved biosynthesis in the non-conventional yeast Kluyveromyces marxianus [0.03%]
工程化碳源响应型启动子以提高非模式酵母马克斯克鲁维酵母中的生物合成水平
Shane Bassett,Nancy A Da Silva
Shane Bassett
Many desired biobased chemicals exhibit a range of toxicity to microbial cell factories, making industry-level biomanufacturing more challenging. Separating microbial growth and production phases is known to be beneficial for improving prod...
Improving 5-(hydroxymethyl)furfural (HMF) tolerance of Pseudomonas taiwanensis VLB120 by automated adaptive laboratory evolution (ALE) [0.03%]
通过自动化适应性实验室进化(ALE)提高假单胞菌台湾株(VLB120)耐受5-羟甲基糠醛(HMF)能力
Thorsten Lechtenberg,Benedikt Wynands,Moritz-Fabian Müller et al.
Thorsten Lechtenberg et al.
The aldehyde 5-(hydroxymethyl)furfural (HMF) is of great importance for a circular bioeconomy. It is a renewable platform chemical that can be converted into a range of useful compounds to replace petroleum-based products such as the green ...
Bioproduction of methylated phenylpropenes and isoeugenol in Escherichia coli [0.03%]
大肠杆菌中生物生产甲基化苯丙烯和异丁香酚
Jeremy Chua,Erik K R Hanko,Andrew Yiakoumetti et al.
Jeremy Chua et al.
Phenylpropenes are a class of natural products that are synthesised by a vast range of plant species and hold considerable promise in the flavour and fragrance industries. Many in vitro studies have been carried out to elucidate and charact...
Engineering of methionine-auxotroph Escherichia coli via parallel evolution of two enzymes from Corynebacterium glutamicum's direct-sulfurylation pathway enables its recovery in minimal medium [0.03%]
通过平行进化两种来自棒状杆菌直接硫酸化途径的酶工程构建赖氨酸缺陷型大肠杆菌使其能在Minimal培养基中回收
Matan Gabay,Inbar Stern,Nadya Gruzdev et al.
Matan Gabay et al.
Methionine biosynthesis relies on the sequential catalysis of multiple enzymes. Escherichia coli, the main bacteria used in research and industry for protein production and engineering, utilizes the three-step trans-sulfurylation pathway ca...