Comparison of orthologous cyanobacterial aldehyde deformylating oxygenases in the production of volatile C3-C7 alkanes in engineered E. coli [0.03%]
比较蓝藻醛脱羰氧酶在工程大肠杆菌中生产易挥发的C3-C7烷烃中的异源表达性能
Pekka Patrikainen,Veronica Carbonell,Kati Thiel et al.
Pekka Patrikainen et al.
Aldehyde deformylating oxygenase (ADO) is a unique enzyme found exclusively in photosynthetic cyanobacteria, which natively converts acyl aldehyde precursors into hydrocarbon products embedded in cellular lipid bilayers. This capacity has o...
Development of a high efficiency integration system and promoter library for rapid modification of Pseudomonas putida KT2440 [0.03%]
恶臭假单胞菌KT2440高效整合体系及启动子库的构建
Joshua R Elmore,Anna Furches,Gara N Wolff et al.
Joshua R Elmore et al.
Pseudomonas putida strains are highly robust bacteria known for their ability to efficiently utilize a variety of carbon sources, including aliphatic and aromatic hydrocarbons. Recently, P. putida has been engineered to valorize the lignin ...
Metabolic engineering of Ustilago trichophora TZ1 for improved malic acid production [0.03%]
代谢工程改造Ustilago trichophoraTZ1用于优化苹果酸生产
Thiemo Zambanini,Hamed Hosseinpour Tehrani,Elena Geiser et al.
Thiemo Zambanini et al.
Ustilago trichophora RK089 has been found recently as a good natural malic acid producer from glycerol. This strain has previously undergone adaptive laboratory evolution for enhanced substrate uptake rate resulting in the strain U. trichop...
Overexpression of the primary sigma factor gene sigA improved carotenoid production by Corynebacterium glutamicum: Application to production of β-carotene and the non-native linear C50 carotenoid bisanhydrobacterioruberin [0.03%]
通过主要的σ因子基因sigA过表达提高棒状杆菌中类胡萝卜素产量：在β-胡萝卜素和非天然C50线性类胡萝卜素二脱水盐菌红素生产中的应用研究
Hironori Taniguchi,Nadja A Henke,Sabine A E Heider et al.
Hironori Taniguchi et al.
Corynebacterium glutamicum shows yellow pigmentation due to biosynthesis of the C50 carotenoid decaprenoxanthin and its glycosides. This bacterium has been engineered for production of various non-native cyclic C40 and C50 carotenoids such ...
Microbial synthesis of a branched-chain ester platform from organic waste carboxylates [0.03%]
利用有机废料合成支链酯平台化合物
Donovan S Layton,Cong T Trinh
Donovan S Layton
Processing of lignocellulosic biomass or organic wastes produces a plethora of chemicals such as short, linear carboxylic acids, known as carboxylates, derived from anaerobic digestion. While these carboxylates have low values and are inhib...
Creating metabolic demand as an engineering strategy in Pseudomonas putida - Rhamnolipid synthesis as an example [0.03%]
芽孢杆菌生产假单胞菌代谢需求的创设作为工程策略-以伪单胞菌产生莱沃酸为例
Till Tiso,Petra Sabelhaus,Beate Behrens et al.
Till Tiso et al.
Metabolic engineering of microbial cell factories for the production of heterologous secondary metabolites implicitly relies on the intensification of intracellular flux directed toward the product of choice. Apart from reactions following ...
System wide cofactor turnovers can propagate metabolic stability between pathways [0.03%]
系统范围的辅因子周转可在途径之间传播代谢稳定性
Y Yang,Y H Guan,J Villadsen
Y Yang
Metabolic homeostasis, or low-level metabolic steady state, has long been taken for granted in metabolic engineering, and research priority has always been given to understand metabolic flux control and regulation of the reaction network. I...
Investigation of useful carbon tracers for 13C-metabolic flux analysis of Escherichia coli by considering five experimentally determined flux distributions [0.03%]
通过考虑五种实验确定的通量分布研究用于大肠杆菌13C代谢流分析的有用碳示踪剂
Kousuke Maeda,Nobuyuki Okahashi,Yoshihiro Toya et al.
Kousuke Maeda et al.
The 13C-MFA experiments require an optimal design since the precision or confidence intervals of the estimated flux levels depends on factors such as the composition of 13C-labeled carbon sources, as well as the metabolic flux distribution ...
Development of a plasmid-based expression system in Clostridium thermocellum and its use to screen heterologous expression of bifunctional alcohol dehydrogenases (adhE s) [0.03%]
芽孢杆菌热纤维梭菌表达系统的开发及其用于异源醇脱氢酶的筛选
Shuen Hon,Anthony A Lanahan,Liang Tian et al.
Shuen Hon et al.
Clostridium thermocellum is a promising candidate for ethanol production from cellulosic biomass, but requires metabolic engineering to improve ethanol yield. A key gene in the ethanol production pathway is the bifunctional aldehyde and alc...
Tet-On lentiviral transductants lose inducibility when silenced for extended intervals in mammary epithelial cells [0.03%]
乳头状上皮细胞的tet-on慢病毒转导物在延长的沉默间隔期间会失去诱导性
Yang Yu,Michelle Montag Lowy,Randolph C Elble
Yang Yu
Silencing of virally transduced genes by promoter methylation and histone deacetylation has been a chronic problem both experimentally and therapeutically. We observed frequent silencing of the tetracycline-inducible Tet-On promoter borne b...