Metabolic engineering of Escherichia coli for the biosynthesis of 2-pyrrolidone [0.03%]
大肠杆菌的代谢工程改造高效生物合成吡咯烷酮新技术研究
Jingwei Zhang,Emily Kao,George Wang et al.
Jingwei Zhang et al.
2-Pyrrolidone is a valuable bulk chemical with myriad applications as a solvent, polymer precursor and active pharmaceutical intermediate. A novel 2-pyrrolidone synthase, ORF27, from Streptomyces aizunensis was identified to catalyze the ri...
Engineering Escherichia coli for the production of terpene mixture enriched in caryophyllene and caryophyllene alcohol as potential aviation fuel compounds [0.03%]
工程改造大肠杆菌高效生产富含没药烯和没药醇的萜类混合物作为潜在航空燃料組分
Weihua Wu,Fang Liu,Ryan W Davis
Weihua Wu
Recent studies have revealed that caryophyllene and its stereoisomers not only exhibit multiple biological activities but also have desired properties as renewable candidates for ground transportation and jet fuel applications. This study p...
Genome-scale model guided design of Propionibacterium for enhanced propionic acid production [0.03%]
泛基因组模型指导丙酸杆菌的菌株设计用于增强丙酸生产
Laura Navone,Tim McCubbin,Ricardo A Gonzalez-Garcia et al.
Laura Navone et al.
Production of propionic acid by fermentation of propionibacteria has gained increasing attention in the past few years. However, biomanufacturing of propionic acid cannot compete with the current oxo-petrochemical synthesis process due to i...
Genome sequence and analysis of Escherichia coli production strain LS5218 [0.03%]
产乳酸菌Escherichia coli LS5218的基因组序列及分析
Jacqueline M Rand,Gina C Gordon,Christopher R Mehrer et al.
Jacqueline M Rand et al.
Escherichia coli strain LS5218 is a useful host for the production of fatty acid derived products, but the genetics underlying this utility have not been fully investigated. Here, we report the genome sequence of LS5218 and a list of large ...
YaliBricks, a versatile genetic toolkit for streamlined and rapid pathway engineering in Yarrowia lipolytica [0.03%]
一种通用的基因工具包YaliBricks在Yarrowia lipolytica中实现通路工程化简单化的研究
Lynn Wong,Jake Engel,Erqing Jin et al.
Lynn Wong et al.
Effective metabolic engineering of microorganisms relies on balanced expression of both heterologous and endogenous genes to channel metabolic flux towards products of interest while achieving reasonable biomass buildup. To facilitate combi...
Metabolic engineering of Schizosaccharomyces pombe via CRISPR-Cas9 genome editing for lactic acid production from glucose and cellobiose [0.03%]
CRISPR-Cas9基因组编辑的产乳酸解淀粉芽孢杆菌发酵生产乳酸
Aiko Ozaki,Rie Konishi,Chisako Otomo et al.
Aiko Ozaki et al.
Modification of the Schizosaccharomyces pombe genome is often laborious, time consuming due to the lower efficiency of homologous recombination. Here, we constructed metabolically engineered S. pombe strains using a CRISPR-Cas9 system and a...
UP Finder: A COBRA toolbox extension for identifying gene overexpression strategies for targeted overproduction [0.03%]
UP_finder：一种COBRA工具箱扩展，用于识别基因过表达策略以实现目标产物的定向过量生产
Xi Wang,Liang Yu,Shulin Chen
Xi Wang
Overexpression of key genes is a basic strategy for overproducing target products via metabolic engineering. Traditionally, identifying those key genes/pathways largely relies on the knowledge of biochemistry and bioinformatics. In this stu...
Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases [0.03%]
异柠檬酸乙醛脱氢酶和异丙醇脱氢酶在Synechocystis PCC 6803中生产异丁醇的研究
Rui Miao,Xufeng Liu,Elias Englund et al.
Rui Miao et al.
Isobutanol is a flammable compound that can be used as a biofuel due to its high energy density and suitable physical and chemical properties. In this study, we examined the capacity of engineered strains of Synechocystis PCC 6803 containin...
A comprehensive evaluation of constraining amino acid biosynthesis in compartmented models for metabolic flux analysis [0.03%]
隔室化模型限制氨基酸生物合成的全面评估用于代谢通量分析
Mathias Lehnen,Birgitta E Ebert,Lars M Blank
Mathias Lehnen
Recent advances in the availability and applicability of genetic tools for non-conventional yeasts have raised high hopes regarding the industrial applications of such yeasts; however, quantitative physiological data on these yeasts, includ...
Eliminating a global regulator of carbon catabolite repression enhances the conversion of aromatic lignin monomers to muconate in Pseudomonas putida KT2440 [0.03%]
在Pseudomonas putida KT2440中消除一个全球性的碳代谢物阻遏调控因子可增强芳香类木质素单体向邻氨基苯甲酸的转化
Christopher W Johnson,Paul E Abraham,Jeffrey G Linger et al.
Christopher W Johnson et al.
Carbon catabolite repression refers to the preference of microbes to metabolize certain growth substrates over others in response to a variety of regulatory mechanisms. Such preferences are important for the fitness of organisms in their na...