Improved sugar-free succinate production by Synechocystis sp. PCC 6803 following identification of the limiting steps in glycogen catabolism [0.03%]
通过鉴定聚糖分解的限制步骤后改进了 Synechocystis sp. PCC 6803 菌株的无糖苹果酸生产性能
Tomohisa Hasunuma,Mami Matsuda,Akihiko Kondo
Tomohisa Hasunuma
Succinate produced by microorganisms can replace currently used petroleum-based succinate but typically requires mono- or poly-saccharides as a feedstock. The cyanobacterium Synechocystis sp. PCC6803 can produce organic acids such as succin...
Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity [0.03%]
通过增加原儿茶酸脱羧酶活性增强葡萄糖和木质素衍生芳香化合物的穆康酸生产效率
Christopher W Johnson,Davinia Salvachúa,Payal Khanna et al.
Christopher W Johnson et al.
The conversion of biomass-derived sugars and aromatic molecules to cis,cis-muconic acid (referred to hereafter as muconic acid or muconate) has been of recent interest owing to its facile conversion to adipic acid, an important commodity ch...
Suresh Sudarsan,Lars M Blank,Alexander Dietrich et al.
Suresh Sudarsan et al.
Soil microorganisms mineralize lignin-derived aromatic carbon sources using oxidative catabolic pathways, such as the β-ketoadipate pathway. Although this aromatic pathway is one of the best-studied pathways in biochemistry, the complete p...
Acidithiobacillus ferrooxidans's comprehensive model driven analysis of the electron transfer metabolism and synthetic strain design for biomining applications [0.03%]
基于铁氧化硫杆菌电子传递代谢的综合性模型驱动分析及生物冶金应用合成菌株设计
Miguel A Campodonico,Daniela Vaisman,Jean F Castro et al.
Miguel A Campodonico et al.
Acidithiobacillus ferrooxidans is a gram-negative chemolithoautotrophic γ-proteobacterium. It typically grows at an external pH of 2 using the oxidation of ferrous ions by oxygen, producing ferric ions and water, while fixing carbon dioxid...
An optimized method for accurate quantification of cell migration using human small intestine cells [0.03%]
一种使用人类小肠细胞精确量化细胞迁移的方法及其优化方法
Steffen Nyegaard,Brian Christensen,Jan Trige Rasmussen
Steffen Nyegaard
Quantifying the ability of a compound to modulate cell migration rate is a crucial part of many studies including those on chemotaxis, wound healing and cancer metastasis. Existing migration assays all have their strengths and weaknesses. T...
Mitochondrial targeting increases specific activity of a heterologous valine assimilation pathway in Saccharomyces cerevisiae [0.03%]
线粒体定位增强异源缬氨酸同化途径在酿酒酵母中的活性
Kevin V Solomon,Elisa Ovadia,Fujio Yu et al.
Kevin V Solomon et al.
Bio-based isobutantol is a sustainable 'drop in' substitute for petroleum-based fuels. However, well-studied production routes, such as the Ehrlich pathway, have yet to be commercialized despite more than a century of research. The more ver...
Interaction of storage carbohydrates and other cyclic fluxes with central metabolism: A quantitative approach by non-stationary 13C metabolic flux analysis [0.03%]
存储碳水化合物及其他循环通量与中心代谢相互作用的定量方法：非稳态同位素示踪代谢流分析法
C A Suarez-Mendez,M Hanemaaijer,Angela Ten Pierick et al.
C A Suarez-Mendez et al.
13C labeling experiments in aerobic glucose limited cultures of Saccharomyces cerevisiae at four different growth rates (0.054; 0.101, 0.207, 0.307 h-1) are used for calculating fluxes that include intracellular cycles (e.g., storage carboh...
Promiscuous plasmid replication in thermophiles: Use of a novel hyperthermophilic replicon for genetic manipulation of Clostridium thermocellum at its optimum growth temperature [0.03%]
耐高温生物体中的质粒复制：一种新型超嗜热性复制子在链球菌热解糖菌最适生长温度下的基因操作中的应用
Joseph Groom,Daehwan Chung,Daniel G Olson et al.
Joseph Groom et al.
Clostridium thermocellum is a leading candidate for the consolidated bioprocessing of lignocellulosic biomass for the production of fuels and chemicals. A limitation to the engineering of this strain is the availability of stable replicatin...
Conversion of levoglucosan and cellobiosan by Pseudomonas putida KT2440 [0.03%]
荧光假单胞菌KT2440催化的莱沃葡萄糖和赛洛二糖的转化
Jeffrey G Linger,Sarah E Hobdey,Mary Ann Franden et al.
Jeffrey G Linger et al.
Pyrolysis offers a straightforward approach for the deconstruction of plant cell wall polymers into bio-oil. Recently, there has been substantial interest in bio-oil fractionation and subsequent use of biological approaches to selectively u...
Hyaluronan production and molecular weight is enhanced in pathway-engineered strains of lactate dehydrogenase-deficient Lactococcus lactis [0.03%]
乳酸脱氢酶缺陷型乳酸链球菌的途径工程菌株能够增强透明质酸的产生及分子量大小
Mandeep Kaur,Guhan Jayaraman
Mandeep Kaur
The potential advantages of hyaluronic acid (HA) production by metabolically-engineered Lactococcus lactis is constrained by the lower molecular weight and yield of HA obtained in these strains, compared to natural producers. Earlier studie...