Conversion and assimilation of furfural and 5-(hydroxymethyl)furfural by Pseudomonas putida KT2440 [0.03%]
恶臭假单胞菌KT2440转化和同化糠醛和5-（羟甲基）糠醛
Michael T Guarnieri,Mary Ann Franden,Christopher W Johnson et al.
Michael T Guarnieri et al.
The sugar dehydration products, furfural and 5-(hydroxymethyl)furfural (HMF), are commonly formed during high-temperature processing of lignocellulose, most often in thermochemical pretreatment, liquefaction, or pyrolysis. Typically, these ...
The expression of glycerol facilitators from various yeast species improves growth on glycerol of Saccharomyces cerevisiae [0.03%]
来自各种酵母的甘油转运蛋白在酿酒酵母中促进甘油生长作用的不同表达研究
Mathias Klein,Zia-Ul Islam,Peter Boldsen Knudsen et al.
Mathias Klein et al.
Glycerol is an abundant by-product during biodiesel production and additionally has several assets compared to sugars when used as a carbon source for growing microorganisms in the context of biotechnological applications. However, most str...
Quantifying complexity in metabolic engineering using the LASER database [0.03%]
利用LASER数据库量化代谢工程的复杂性
James D Winkler,Andrea L Halweg-Edwards,Ryan T Gill
James D Winkler
We previously introduced the LASER database (Learning Assisted Strain EngineeRing, https://bitbucket.org/jdwinkler/laser_release) (Winkler et al. 2015) to serve as a platform for understanding past and present metabolic engineering practice...
Computational metabolic engineering strategies for growth-coupled biofuel production by Synechocystis [0.03%]
用于Synechocystis生物燃料生产的生长偶联代谢工程的计算策略
Kiyan Shabestary,Elton P Hudson
Kiyan Shabestary
Chemical and fuel production by photosynthetic cyanobacteria is a promising technology but to date has not reached competitive rates and titers. Genome-scale metabolic modeling can reveal limitations in cyanobacteria metabolism and guide ge...
Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources [0.03%]
利用还原性硫化物作为能源增强全自养工程菌产脂肪酸能力
Harry R Beller,Peng Zhou,Talia N M Jewell et al.
Harry R Beller et al.
Chemolithoautotrophic bacteria that oxidize reduced sulfur compounds, such as H2S, while fixing CO2 are an untapped source of renewable bioproducts from sulfide-laden waste, such as municipal wastewater. In this study, we report engineering...
The impact of respiration and oxidative stress response on recombinant α-amylase production by Saccharomyces cerevisiae [0.03%]
呼吸作用及氧化应激响应对酿酒酵母分泌重组α-淀粉酶的影响机制研究
José L Martínez,Eugenio Meza,Dina Petranovic et al.
José L Martínez et al.
Studying protein production is important for fundamental research on cell biology and applied research for biotechnology. Yeast Saccharomyces cerevisiae is an attractive workhorse for production of recombinant proteins as it does not secret...
Molecular Cloning Designer Simulator (MCDS): All-in-one molecular cloning and genetic engineering design, simulation and management software for complex synthetic biology and metabolic engineering projects [0.03%]
分子克隆设计器模拟器（MCDS）：综合型分子克隆及基因工程设计、仿真和管理软件，应用于复杂的合成生物学和代谢工程研究项目
Zhenyu Shi,Claudia E Vickers
Zhenyu Shi
Molecular Cloning Designer Simulator (MCDS) is a powerful new all-in-one cloning and genetic engineering design, simulation and management software platform developed for complex synthetic biology and metabolic engineering projects. In addi...
A genetic screen for increasing metabolic flux in the isoprenoid pathway of Saccharomyces cerevisiae: Isolation of SPT15 mutants using the screen [0.03%]
通过遗传筛选在酿酒酵母异戊二烯途径中增加代谢流：使用筛选分离SPT15突变体
M Wadhwa,A K Bachhawat
M Wadhwa
A genetic screen to identify mutants that can increase flux in the isoprenoid pathway of yeast has been lacking. We describe a carotenoid-based visual screen built with the core carotenogenic enzymes from the red yeast Rhodosporidium torulo...
Improving the flux distributions simulated with genome-scale metabolic models of Saccharomyces cerevisiae [0.03%]
提高基因组规模代谢模型模拟的酿酒酵母辅因子分布精度
Rui Pereira,Jens Nielsen,Isabel Rocha
Rui Pereira
Genome-scale metabolic models (GEMs) can be used to evaluate genotype-phenotype relationships and their application to microbial strain engineering is increasing in popularity. Some of the algorithms used to simulate the phenotypes of mutan...
The Saccharomyces cerevisiae pheromone-response is a metabolically active stationary phase for bio-production [0.03%]
酿酒酵母的交配反应是一种代谢活跃的静止阶段用于生物制造
Thomas C Williams,Bingyin Peng,Claudia E Vickers et al.
Thomas C Williams et al.
The growth characteristics and underlying metabolism of microbial production hosts are critical to the productivity of metabolically engineered pathways. Production in parallel with growth often leads to biomass/bio-product competition for ...