Simultaneous biosynthesis of (R)-acetoin and ethylene glycol from D-xylose through in vitro metabolic engineering [0.03%]
体外代谢工程法同步生物合成(Ｒ-)丙酮酸和乙二醇的研究——以木糖为原料
Xiaojing Jia,Robert M Kelly,Yejun Han
Xiaojing Jia
(R)-acetoin is a four-carbon platform compound used as the precursor for synthesizing novel optically active materials. Ethylene glycol (EG) is a large-volume two-carbon commodity chemical used as the anti-freezing agent and building-block ...
Engineering redox-balanced ethanol production in the cellulolytic and extremely thermophilic bacterium, Caldicellulosiruptor bescii [0.03%]
在纤维素降解的极热嗜菌体Caldicellulosiruptor bescii中打造还原电位平衡的乙醇生产体系
Amanda M Williams-Rhaesa,Gabriel M Rubinstein,Israel M Scott et al.
Amanda M Williams-Rhaesa et al.
Caldicellulosiruptor bescii is an extremely thermophilic cellulolytic bacterium with great potential for consolidated bioprocessing of renewable plant biomass. Since it does not natively produce ethanol, metabolic engineering is required to...
Identification of parallel and divergent optimization solutions for homologous metabolic enzymes [0.03%]
同源代谢酶的并行和分歧优化解的识别
Robert F Standaert,Richard J Giannone,Joshua K Michener
Robert F Standaert
Metabolic pathway assembly typically involves the expression of enzymes from multiple organisms in a single heterologous host. Ensuring that each enzyme functions effectively can be challenging, since many potential factors can disrupt prop...
A novel chimaeric flocculation protein enhances flocculation in Saccharomyces cerevisiae [0.03%]
一种新颖的嵌合絮凝蛋白可增强假丝酵母属锁状联合缺陷素的絮凝能力
Johan O Westman,Jonas Nyman,Richard M A Manara et al.
Johan O Westman et al.
Yeast flocculation is the reversible formation of multicellular complexes mediated by lectin-like cell wall proteins binding to neighbouring cells. Strong flocculation can improve the inhibitor tolerance and fermentation performance of yeas...
Alleviation of reactive oxygen species enhances PUFA accumulation in Schizochytrium sp. through regulating genes involved in lipid metabolism [0.03%]
通过调节脂质代谢相关基因促进Schizochytrium sp.中多不饱和脂肪酸积累的氧化应激缓解机制
Sai Zhang,Yaodong He,Biswarup Sen et al.
Sai Zhang et al.
The unicellular heterotrophic thraustochytrids are attractive candidates for commercial polyunsaturated fatty acids (PUFA) production. However, the reactive oxygen species (ROS) generated in their aerobic fermentation process often limits t...
Metabolic engineering of Saccharomyces cerevisiae for overproduction of triacylglycerols [0.03%]
代谢工程学改造酿酒酵母用于过量产生三酰甘油油脂分泌菌产油研究
Raphael Ferreira,Paulo Gonçalves Teixeira,Michael Gossing et al.
Raphael Ferreira et al.
Triacylglycerols (TAGs) are valuable versatile compounds that can be used as metabolites for nutrition and health, as well as feedstocks for biofuel production. Although Saccharomyces cerevisiae is the favored microbial cell factory for ind...
A protocatechuate biosensor for Pseudomonas putida KT2440 via promoter and protein evolution [0.03%]
通过启动子和蛋白质进化构建恶稿酸生物传感器检测假单胞菌KT2440
Ramesh K Jha,Jeremy M Bingen,Christopher W Johnson et al.
Ramesh K Jha et al.
Robust fluorescence-based biosensors are emerging as critical tools for high-throughput strain improvement in synthetic biology. Many biosensors are developed in model organisms where sophisticated synthetic biology tools are also well esta...
Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae [0.03%]
利用工程酵母由葡萄糖生物合成己二酸
Kaushik Raj,Siavash Partow,Kevin Correia et al.
Kaushik Raj et al.
Adipic acid is an important industrial chemical used in the synthesis of nylon-6,6. The commercial synthesis of adipic acid uses petroleum-derived benzene and releases significant quantities of greenhouse gases. Biocatalytic production of a...
Selection Finder (SelFi): A computational metabolic engineering tool to enable directed evolution of enzymes [0.03%]
选择性查找器（SelFi）：一种计算型代谢工程工具，用于使酶的定向进化成为可能
Neda Hassanpour,Ehsan Ullah,Mona Yousofshahi et al.
Neda Hassanpour et al.
Directed evolution of enzymes consists of an iterative process of creating mutant libraries and choosing desired phenotypes through screening or selection until the enzymatic activity reaches a desired goal. The biggest challenge in directe...
Synechocystis PCC 6803 overexpressing RuBisCO grow faster with increased photosynthesis [0.03%]
Rubisco过表达使Synechocystis PCC 6803生长速度加快且光合作用增强
Feiyan Liang,Peter Lindblad
Feiyan Liang
The ribulose-1,5-bisphosphate (RuBP) oxygenation reaction catalyzed by Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is competing with carboxylation, being negative for both energy and carbon balances in photoautotrophic organis...