Substrate stiffness-dependent metabolic reprogramming of iPSC-derived cardiomyocytes on physiological PDMS polymers [0.03%]
生理级PDMS聚合物上基底刚度依赖的iPSC来源心肌细胞代谢重编程
Leena Patel,Bryan P Marzullo,Jonathan Barlow et al.
Leena Patel et al.
Many cardiac pathologies are characterised by increased stiffness of the myocardium, due to excess deposition of extracellular matrix (ECM) proteins and structural remodelling, impacting the behaviour of cardiomyocytes (CMs). Metabolism of ...
Improving recombinant protein secretion in Aspergillus nidulans by targeting the N-glycosylation machinery [0.03%]
通过靶向N-糖基化装置来改善产黄曲霉中重组蛋白的分泌
Jaqueline Aline Gerhardt,Marcelo Ventura Rubio,Cesar Rafael Fanchini Terrasan et al.
Jaqueline Aline Gerhardt et al.
Filamentous fungi are cell factories traditionally used for enzyme production in various industrial sectors, including food and beverages, biopolymers, biofuels, and animal feed. Despite significant progress in optimizing enzyme production,...
Multi-step pathway engineering in probiotic Saccharomyces boulardii for abscisic acid production in the gut [0.03%]
用于肠道脱落酸生产的益生菌Saccharomyces boulardii多步途径工程化
Femke Van Gaever,Paul Vandecruys,Yasmine Driege et al.
Femke Van Gaever et al.
The plant hormone abscisic acid (ABA) has gained attention for its role in animals and humans, particularly due to its protective effects in various immune and inflammatory disorders. Given its high concentrations in fruits like figs, bilbe...
Improvement of D-lactic acid production from methanol by metabolically engineered Komagataella phaffii via ultra-violet mutagenesis [0.03%]
通过紫外线诱变优化甲醇代谢工程解脂耶罗华氏酵母生产D-乳酸性能
Yoshifumi Inoue,Kaito Nakamura,Ryosuke Yamada et al.
Yoshifumi Inoue et al.
Methanol has attracted attention as an alternative carbon source to petroleum. Komagataella phaffii, a methanol-assimilating yeast, is a useful host for the chemical production from methanol. A previous study successfully constructed a meta...
13C-metabolic flux analysis of Saccharomyces cerevisiae in complex media [0.03%]
酿酒酵母在复杂培养基中的13C-代谢流分析
Hayato Fujiwara,Nobuyuki Okahashi,Taisuke Seike et al.
Hayato Fujiwara et al.
Saccharomyces cerevisiae is often cultivated in complex media for applications in food and other biochemical production. However, 13C-metabolic flux analysis (13C-MFA) has been conducted for S. cerevisiae cultivated in synthetic media, resu...
Engineering Pseudomonas putida for production of 3-hydroxyacids using hybrid type I polyketide synthases [0.03%]
工程改造恶臭假单胞菌利用混合型I型聚酮合酶生产3-羟基酸类化合物
Matthias Schmidt,Aaron A Vilchez,Namil Lee et al.
Matthias Schmidt et al.
Engineered type I polyketide synthases (T1PKSs) are a potentially transformative platform for the biosynthesis of small molecules. Due to their modular nature, T1PKSs can be rationally designed to produce a wide range of bulk or specialty c...
Production of borneol, camphor, and bornyl acetate using engineered Saccharomyces cerevisiae [0.03%]
利用工程酵母生产冰片、薄荷脑和乙酸龙脑酯
Masahiro Tominaga,Kazuma Kawakami,Hiro Ogawa et al.
Masahiro Tominaga et al.
Microbial production of bicyclic monoterpenes is of great interest because their production primarily utilizes non-sustainable resources. Here, we report an engineered Saccharomyces cerevisiae yeast that produces bicyclic monoterpenes, incl...
Synthetic pathways for microbial biosynthesis of valuable pyrazine derivatives using genetically modified Pseudomonas putida KT2440 [0.03%]
基于基因工程的肺炎克雷伯氏菌KT2440合成芳香族吡嗪类化合物代谢途径的研究
Vytautas Petkevičius,Justė Juknevičiūtė,Domas Mašonis et al.
Vytautas Petkevičius et al.
Using engineered microbes for synthesizing high-valued chemicals from renewable sources is a foundation in synthetic biology, however, it is still in its early stages. Here, we present peculiarities and troubleshooting of the construction o...
Metabolic growth-coupling strategies for in vivo enzyme selection systems [0.03%]
用于体内酶选择系统的代谢偶联生长策略
Tobias B Alter,Pascal A Pieters,Colton J Lloyd et al.
Tobias B Alter et al.
Whole-cell biocatalysis facilitates the production of a wide range of industrially and pharmaceutically relevant molecules from sustainable feedstocks such as plastic wastes, carbon dioxide, lignocellulose, or plant-based sugar sources. The...
Michael Weldon,Sanniv Ganguly,Christian Euler
Michael Weldon
The growing plastics end-of-life crisis threatens ecosystems and human health globally. Microbial plastic degradation and upcycling have emerged as potential solutions to this complex challenge, but their industrial feasibility and limitati...