Genomic and proteomic biases inform metabolic engineering strategies for anaerobic fungi [0.03%]
基因组和蛋白质组偏差可指导厌氧真菌的代谢工程策略
St Elmo Wilken,Susanna Seppälä,Thomas S Lankiewicz et al.
St Elmo Wilken et al.
Anaerobic fungi (Neocallimastigomycota) are emerging non-model hosts for biotechnology due to their wealth of biomass-degrading enzymes, yet tools to engineer these fungi have not yet been established. Here, we show that the anaerobic gut f...
Pathway enzyme engineering for flavonoid production in recombinant microbes [0.03%]
重组微生物中黄酮类化合物合成途径酶工程化研究
Jian Zha,Xia Wu,Guoli Gong et al.
Jian Zha et al.
Metabolic engineering of microbial strains for the production of flavonoids of industrial interest has attracted great attention due to its promising advantages over traditional extraction approaches, such as independence of plantation, fac...
Contextualized genome-scale model unveils high-order metabolic effects of the specific growth rate and oxygenation level in recombinant Pichia pastoris [0.03%]
基于生长速率和氧合水平的毕赤酵母重组菌株代谢组学模型
Paulina Torres,Pedro A Saa,Joan Albiol et al.
Paulina Torres et al.
Pichia pastoris is recognized as a biotechnological workhorse for recombinant protein expression. The metabolic performance of this microorganism depends on genetic makeup and culture conditions, amongst which the specific growth rate and o...
Targeted enzyme gene re-positioning: A computational approach for discovering alternative bacterial enzymes for the synthesis of plant-specific secondary metabolites [0.03%]
靶向酶基因重新定位：一种计算方法，用于发现合成植物特异性次级代谢物的替代细菌酶
Yuya Nakamura,Shuichi Hirose,Yuko Taniguchi et al.
Yuya Nakamura et al.
Plant-biosynthesised secondary metabolites are unique sources of pharmaceuticals, food additives, and flavourings, among other industrial uses. However, industrial production of these metabolites is difficult because of their structural com...
A comprehensive genome-scale model for Rhodosporidium toruloides IFO0880 accounting for functional genomics and phenotypic data [0.03%]
基于功能基因组学和表型数据的毕赤酵母IFO0880全面的基因组规模模型
Hoang V Dinh,Patrick F Suthers,Siu Hung Joshua Chan et al.
Hoang V Dinh et al.
Rhodosporidium toruloides is a red, basidiomycetes yeast that can accumulate a large amount of lipids and produce carotenoids. To better assess this non-model yeast's metabolic capabilities, we reconstructed a genome-scale model of R. torul...
Corrigendum to "YaliBricks, a versatile genetic toolkit for streamlined and rapid pathway engineering in Yarrowia lipolytica" [0.03%]
对“YaliBricks：一种用于Yarrowia lipolytica通路工程的多功能遗传工具包”的勘误注释
Lynn Wong,Jake Engel,Erqing Jin et al.
Lynn Wong et al.
[This corrects the article DOI: 10.1016/j.meteno.2017.09.001.]. © 2017 The Author(s).
Published Erratum
Metabolic engineering communications. 2019 Aug 13:9:e00099. DOI:10.1016/j.mec.2019.e00099 2019
Omics-driven identification and elimination of valerolactam catabolism in Pseudomonas putida KT2440 for increased product titer [0.03%]
基于组学的假单胞菌KT2440降解途径表征及工程改造以提高产物滴度
Mitchell G Thompson,Luis E Valencia,Jacquelyn M Blake-Hedges et al.
Mitchell G Thompson et al.
Pseudomonas putida is a promising bacterial chassis for metabolic engineering given its ability to metabolize a wide array of carbon sources, especially aromatic compounds derived from lignin. However, this omnivorous metabolism can also be...
Application of a genome-scale model in tandem with enzyme assays for identification of metabolic signatures of high and low CHO cell producers [0.03%]
联合应用基因组范围模型和酶分析鉴定高产和低产CHO细胞的代谢标志物
Cyrielle Calmels,Solène Arnoult,Bassem Ben Yahia et al.
Cyrielle Calmels et al.
Biopharmaceutical industrial processes are based on high yielding stable recombinant Chinese Hamster Ovary (CHO) cells that express monoclonal antibodies. However, the process and feeding regimes need to be adapted for each new cell line, a...
Overproduction of gentamicin B in industrial strain Micromonospora echinospora CCTCC M 2018898 by cloning of the missing genes genR and genS [0.03%]
通过克隆缺失的基因genR和genS提高工业生产菌Micromonospora echinospora CCTCC M 2018898中吉他霉素B产量
Yingying Chang,Baozhong Chai,Yunkun Ding et al.
Yingying Chang et al.
In pharmaceutical industry, isepamicin is mainly manufactured from gentamicin B, which is produced by Micromonospora echinospora as a minor component of the gentamicin complex. Improvement of gentamicin B production through metabolic engine...
Advances in the development and application of microbial consortia for metabolic engineering [0.03%]
代谢工程中微生物菌剂的研发及应用进展
Kamran Jawed,Syed Shams Yazdani,Mattheos Ag Koffas
Kamran Jawed
Recent advances in metabolic engineering enable the production of high-value chemicals via expressing complex biosynthetic pathways in a single microbial host. However, many engineered strains suffer from poor product yields due to redox im...