Debottlenecking mevalonate pathway for antimalarial drug precursor amorphadiene biosynthesis in Yarrowia lipolytica [0.03%]
利用酿酒酵母丝孢菌优化抗疟药物前体牻牛儿烯生物合成途径中的异戊二烯代谢路径
Monireh Marsafari,Peng Xu
Monireh Marsafari
World Health Organization reports that half of the population in developing countries are at risk of malaria infection. Artemisinin, the most potent anti-malaria drug, is a sesquiterpene endoperoxide extracted from the plant Artemisia annua...
A cell-free system for production of 2,3-butanediol is robust to growth-toxic compounds [0.03%]
一种用于生产2,3-丁二醇的无细胞系统可以耐受生长抑制性化合物
Jennifer E Kay,Michael C Jewett
Jennifer E Kay
The need for sustainable, low-cost production of bioenergy and commodity chemicals is increasing. Unfortunately, the engineering potential of whole-cell catalysts to address this need can be hampered by cellular toxicity. When such bottlene...
Evaluation of freely available software tools for untargeted quantification of 13C isotopic enrichment in cellular metabolome from HR-LC/MS data [0.03%]
评估免费软件工具的性能：从高分辨LC/MS数据中定量细胞代谢物中碳同位素丰度
Manohar C Dange,Vivek Mishra,Bratati Mukherjee et al.
Manohar C Dange et al.
13C Metabolic Flux Analysis (13C-MFA) involves the quantification of isotopic enrichment in cellular metabolites and fitting the resultant data to the metabolic network model of the organism. Coverage and resolution of the resultant flux ma...
Cheng Li,Charles A Swofford,Anthony J Sinskey
Cheng Li
There is an increasing demand for carotenoids due to their applications in the food, flavor, pharmaceutical and feed industries, however, the extraction and synthesis of these compounds can be expensive and technically challenging. Microbia...
Improving heterologous protein expression in Synechocystis sp. PCC 6803 for alpha-bisabolene production [0.03%]
提高异源蛋白质在Synechocystis sp。PCC 6803中表达以促进α-倍半松油烯的生成
Jacob Sebesta,Christie Am Peebles
Jacob Sebesta
Cyanobacterial biofuels have the potential to reduce the cost and climate impacts of biofuel production because primary carbon fixation and conversion to fuel are completed together in the cultivation of the cyanobacteria. Cyanobacterial bi...
Development of both type I-B and type II CRISPR/Cas genome editing systems in the cellulolytic bacterium Clostridium thermocellum [0.03%]
纤维素分解细菌热纤梭菌(I-B型和II型CRISPR/Cas基因组编辑系统的发展)
Julie E Walker,Anthony A Lanahan,Tianyong Zheng et al.
Julie E Walker et al.
The robust lignocellulose-solubilizing activity of C. thermocellum makes it a top candidate for consolidated bioprocessing for biofuel production. Genetic techniques for C. thermocellum have lagged behind model organisms thus limiting attem...
Production of tetra-methylpyrazine using engineered Corynebacterium glutamicum [0.03%]
使用工程化谷氨酸棒杆菌生产四甲基吡嗪
Thomas Eng,Yusuke Sasaki,Robin A Herbert et al.
Thomas Eng et al.
Corynebacterium glutamicum ATCC 13032 is an established and industrially-relevant microbial host that has been utilized for the expression of many desirable bioproducts. Tetra-methylpyrazine (TMP) is a naturally occurring alkylpyrazine with...
CRISPR-Cas12a/Cpf1-assisted precise, efficient and multiplexed genome-editing in Yarrowia lipolytica [0.03%]
基于CRISPR-Cas12a/Cpf1的酿酒汉逊酵母基因组精准高效多靶点编辑系统
Zhiliang Yang,Harley Edwards,Peng Xu
Zhiliang Yang
CRISPR-Cas9 has been widely adopted as the basic toolkit for precise genome-editing and engineering in various organisms. Alternative to Cas9, Cas12 or Cpf1 uses a simple crRNA as a guide and expands the protospacer adjacent motif (PAM) seq...
De novo biosynthesis of 8-hydroxyoctanoic acid via a medium-chain length specific fatty acid synthase and cytochrome P450 in Saccharomyces cerevisiae [0.03%]
通过中链脂肪酸合成酶和细胞色素P450从头合成8-羟基辛酸的研究Saccharomyces cerevisiae中的
Florian Wernig,Eckhard Boles,Mislav Oreb
Florian Wernig
Terminally hydroxylated fatty acids or dicarboxylic acids are industrially relevant compounds with broad applications. Here, we present the proof of principle for the de novo biosynthesis of 8-hydroxyoctanoic acid from glucose and ethanol i...
Developing E. coli-E. coli co-cultures to overcome barriers of heterologous tryptamine biosynthesis [0.03%]
开发E. coli-E. coli共培养体系以克服异源色胺生物合成的障碍
Xiaonan Wang,Lizelle Policarpio,Dhara Prajapati et al.
Xiaonan Wang et al.
Tryptamine is an alkaloid compound with demonstrated bioactivities and is also a precursor molecule to many important hormones and neurotransmitters. The high efficiency biosynthesis of tryptamine from inexpensive and renewable carbon subst...