NetFlow: A tool for isolating carbon flows in genome-scale metabolic networks [0.03%]
基于基因组规模代谢网络的碳流分离工具-netflow算法研究
Sean G Mack,Ganesh Sriram
Sean G Mack
Genome-scale stoichiometric models (GSMs) have been widely utilized to predict and understand cellular metabolism. GSMs and the flux predictions resulting from them have proven indispensable to fields ranging from metabolic engineering to h...
Engineering of a new Escherichia coli strain efficiently metabolizing cellobiose with promising perspectives for plant biomass-based application design [0.03%]
工程构建高效利用半乳糖的新大肠杆菌菌株及其在基于植物生物质应用设计中的前景研究
Romain Borne,Nicolas Vita,Nathalie Franche et al.
Romain Borne et al.
The necessity to decrease our fossil energy dependence requests bioprocesses based on biomass degradation. Cellobiose is the main product released by cellulases when acting on the major plant cell wall polysaccharide constituent, the cellul...
Engineering precursor pools for increasing production of odd-chain fatty acids in Yarrowia lipolytica [0.03%]
利用脂肪酸前体池工程提高脂卵囊菌中奇数链脂肪酸的产量
Young-Kyoung Park,Florence Bordes,Fabien Letisse et al.
Young-Kyoung Park et al.
Microbial production of lipids is one of the promising alternatives to fossil resources with increasing environmental and energy concern. Odd-chain fatty acids (OCFA), a type of unusual lipids, are recently gaining a lot of interest as targ...
Novel allosteric inhibition of phosphoribulokinase identified by ensemble kinetic modeling of Synechocystis sp. PCC 6803 metabolism [0.03%]
通过集合动力学建模在热蓝细菌Synechocystis sp. PCC 6803中鉴定出新的别构磷酸核酮糖激酶抑制剂
Hiroki Nishiguchi,James Liao,Hiroshi Shimizu et al.
Hiroki Nishiguchi et al.
The present study attempted a computer simulation of the metabolism of a model cyanobacteria, Synechocystis sp. PCC 6803 (PCC 6803) to predict allosteric inhibitions that are likely to occur in photoautotrophic and mixotrophic conditions as...
Improving the homologous recombination efficiency of Yarrowia lipolytica by grafting heterologous component from Saccharomyces cerevisiae [0.03%]
通过嫁接假丝酵母基因组组件提高解脂耶氏酵母同源重组效率
Qingchun Ji,Jie Mai,Ying Ding et al.
Qingchun Ji et al.
The oleaginous non-conventional yeast Yarrowia lipolytica has enormous potential as a microbial platform for the synthesis of various bioproducts. However, while the model yeast Saccharomyces cerevisiae has very high homologous recombinatio...
Pathway engineering of Escherichia coli for one-step fermentative production of L-theanine from sugars and ethylamine [0.03%]
以糖和单乙胺为原料的一锅法发酵生产L-茶氨酸的代谢工程菌株构建及性能优化
Xiaoguang Fan,Tong Zhang,Yuanqing Ji et al.
Xiaoguang Fan et al.
L-theanine is the most abundant free amino acid in tea that offers various favorable physiological and pharmacological effects. Bacterial enzyme of γ-glutamylmethylamide synthetase (GMAS) can catalyze the synthesis of theanine from glutama...
A unified design allows fine-tuning of biosensor parameters and application across bacterial species [0.03%]
统一设计允许细菌物种的生物传感器参数和应用的精细调整
Christiane Katharina Sonntag,Lion Konstantin Flachbart,Celine Maass et al.
Christiane Katharina Sonntag et al.
In recent years, transcriptional biosensors have become valuable tools in metabolic engineering as they allow semiquantitative determination of metabolites in single cells. Although being perfectly suitable tools for high-throughput screeni...
Genome-scale metabolic reconstruction of the non-model yeast Issatchenkia orientalis SD108 and its application to organic acids production [0.03%]
Issatchenkia orientalis SD108 全基因组代谢重建及其在有机酸生产中的应用
Patrick F Suthers,Hoang V Dinh,Zia Fatma et al.
Patrick F Suthers et al.
Many platform chemicals can be produced from renewable biomass by microorganisms, with organic acids making up a large fraction. Intolerance to the resulting low pH growth conditions, however, remains a challenge for the industrial producti...
Characterization of Met25 as a color associated genetic marker in Yarrowia lipolytica [0.03%]
Met25作为耶氏肺孢子菌中与颜色相关遗传标志物的特征分析
Harley Edwards,Zhiliang Yang,Peng Xu
Harley Edwards
Yarrowia lipolytica offers an ideal host for biosynthesis of high value natural products and oleochemicals through metabolic engineering despite being restricted to a limited number of selective markers, and counter-selection achieved prima...
Systems biology approaches integrated with artificial intelligence for optimized metabolic engineering [0.03%]
集成人工智能的系统生物学方法用于优化代谢工程
Mohamed Helmy,Derek Smith,Kumar Selvarajoo
Mohamed Helmy
Metabolic engineering aims to maximize the production of bio-economically important substances (compounds, enzymes, or other proteins) through the optimization of the genetics, cellular processes and growth conditions of microorganisms. Thi...