Integrated laboratory evolution and rational engineering of GalP/Glk-dependent Escherichia coli for higher yield and productivity of L-tryptophan biosynthesis [0.03%]
融合实验室进化和理性工程整合GalP / Glk依赖性大肠杆菌以提高L色氨酸生物合成的产量和生产力
Chen Minliang,Ma Chengwei,Chen Lin et al.
Chen Minliang et al.
L-Tryptophan (Trp) is a high-value aromatic amino acid with diverse applications in food and pharmaceutical industries. Although production of Trp by engineered Escherichia coli has been extensively studied, the need of multiple precursors ...
2A-linked bi-, tri-, and quad-cistrons for the stepwise biosynthesis of β-carotene, zeaxanthin, and ketocarotenoids in rice endosperm [0.03%]
用于水稻胚乳中β-胡萝卜素、角黄素和酮类类胡萝卜素分步生物合成的2A连接双顺合基因、三顺合基因和四顺合基因体系
Ye Sol Jeong,Hyung-Keun Ku,Young-Joo Jung et al.
Ye Sol Jeong et al.
Foot-and-mouth disease virus (FMDV) 2A constructs have been successfully used for the production of "Golden Rice", a β-carotene producing rice strain. However, to allay public fears and opposition to plants carrying a mammalian pathogenic ...
Engineering Ustilago maydis for production of tailor-made mannosylerythritol lipids [0.03%]
改良产瘤柄囊腔菌用于生产定制化的甘cerythritol脂质体
Fabienne Becker,Thorsten Stehlik,Uwe Linne et al.
Fabienne Becker et al.
Mannosylerythritol lipids (MELs) are surface active glycolipids secreted by various fungi. MELs can be used as biosurfactants and are a biodegradable resource for the production of detergents or pharmaceuticals. Different fungal species syn...
Enhanced limonene production in a fast-growing cyanobacterium through combinatorial metabolic engineering [0.03%]
通过组合代谢工程在快速生长的蓝藻中增强蒈烯生成
Po-Cheng Lin,Fuzhong Zhang,Himadri B Pakrasi
Po-Cheng Lin
Terpenoids are a large and diverse group of natural products with commercial applications. Microbial production of terpenes is considered as a feasible approach for the stable supply of these complex hydrocarbons. Cyanobacteria, photosynthe...
Isobutene production in Synechocystis sp. PCC 6803 by introducing α-ketoisocaproate dioxygenase from Rattus norvegicus [0.03%]
利用大鼠α-酮异己酸氧化酶在集胞蓝细菌中生产异丁烯
Henna Mustila,Amit Kugler,Karin Stensjö
Henna Mustila
Cyanobacteria can be utilized as a platform for direct phototrophic conversion of CO2 to produce several types of carbon-neutral biofuels. One promising compound to be produced photobiologically in cyanobacteria is isobutene. As a volatile ...
A lysate proteome engineering strategy for enhancing cell-free metabolite production [0.03%]
一种工程细胞裂解物蛋白组以增强体外代谢物合成的策略
David C Garcia,Jaime Lorenzo N Dinglasan,Him Shrestha et al.
David C Garcia et al.
Cell-free systems present a significant opportunity to harness the metabolic potential of diverse organisms. Removing the cellular context provides the ability to produce biological products without the need to maintain cell viability and e...
Engineered cyanobacteria with additional overexpression of selected Calvin-Benson-Bassham enzymes show further increased ethanol production [0.03%]
过表达卡尔文循环中某些酶的工程蓝藻产乙醇性能进一步提升
Stamatina Roussou,Alessia Albergati,Feiyan Liang et al.
Stamatina Roussou et al.
Cyanobacteria are one of the most promising microorganisms to produce biofuels and renewable chemicals due to their oxygenic autotrophic growth properties. However, to rely on photosynthesis, which is one of the main reasons for slow growth...
Trans-4-hydroxy-L-proline production by the cyanobacterium Synechocystis sp. PCC 6803 [0.03%]
异养蓝藻生产反式-4-羟基-L-脯氨酸
Fabian Brandenburg,Eleni Theodosiou,Carolin Bertelmann et al.
Fabian Brandenburg et al.
Cyanobacteria play an important role in photobiotechnology. Yet, one of their key central metabolic pathways, the tricarboxylic acid (TCA) cycle, has a unique architecture compared to most heterotrophs and still remains largely unexploited....
The identification of novel promoters and terminators for protein expression and metabolic engineering applications in Kluyveromyces marxianus [0.03%]
毕赤酵母甲壳菌马克斯氏变种中新型启动子和终止子的鉴定及其在蛋白质表达与代谢工程中的应用
Pradeep Kumar,Debendra Kumar Sahoo,Deepak Sharma
Pradeep Kumar
The K. marxianus has emerged as a potential yeast strain for various biotechnological applications. However, the limited number of available genetic tools has hindered the widespread usage of this yeast. In the current study we have expande...
Metabolic engineering of Synechocystis sp. PCC 6803 for improved bisabolene production [0.03%]
用于提高角鲨烯生产的Synechocystis sp. PCC 6803代谢工程菌株的构建
João S Rodrigues,Pia Lindberg
João S Rodrigues
Terpenoids are a wide class of organic compounds with industrial relevance. The natural ability of cyanobacteria to produce terpenoids via the methylerythritol 4-phosphate (MEP) pathway makes these organisms appealing candidates for the gen...