CRISPR-Cas system enables fast and simple genome editing of industrial Saccharomyces cerevisiae strains [0.03%]
CRISPR-Cas系统可实现工业酿酒酵母菌株的快速简便基因组编辑
Vratislav Stovicek,Irina Borodina,Jochen Forster
Vratislav Stovicek
There is a demand to develop 3rd generation biorefineries that integrate energy production with the production of higher value chemicals from renewable feedstocks. Here, robust and stress-tolerant industrial strains of Saccharomyces cerevis...
Development of a plasmid addicted system that is independent of co-inducers, antibiotics and specific carbon source additions for bioproduct (1-butanol) synthesis in Escherichia coli [0.03%]
开发一种无需共诱导物、抗生素和特定碳源添加的质粒成瘾系统，用于大肠杆菌中生物产品的（丁醇）合成
Rick Laguna,Sarah J Young,Chih-Chin Chen et al.
Rick Laguna et al.
Synthetic biology approaches for the synthesis of value-based products provide interesting and potentially fruitful possibilities for generating a wide variety of useful compounds and biofuels. However, industrial production is hampered by ...
Ryan T Gill,Vassily Hatzimanikatis
Ryan T Gill
Tailoring strain construction strategies for muconic acid production in S. cerevisiae and E. coli [0.03%]
针对酿酒酵母和大肠杆菌合成穆聪酸的菌株构建策略优化
Nils J H Averesch,Jens O Krömer
Nils J H Averesch
There is currently a strong interest to derive the biological precursor cis,cis-muconic acid from shikimate pathway-branches to develop a biological replacement for adipic acid. Pioneered by the Frost laboratory this concept has regained in...
Model-driven identification of dosing regimens that maximize the antimicrobial activity of nitric oxide [0.03%]
基于模型的剂量方案识别可最大化一氧化氮抗菌活性的方法
Jonathan L Robinson,Richard V Miller,Mark P Brynildsen
Jonathan L Robinson
The antimicrobial properties of nitric oxide (NO●) have motivated the design of NO●-releasing materials for the treatment and prevention of infection. The biological activity of NO● is dependent on its delivery rate, suggesting that vari...
Surfactin, a quorum sensing signal molecule, globally affects the carbon metabolism in Bacillus amyloliquefaciens [0.03%]
表面活性素通过全局调控碳代谢途径抑制枯草芽孢杆菌的群体感应信号响应
Jiahong Wen,Xiuyun Zhao,Fengmei Si et al.
Jiahong Wen et al.
Surfactin, a quorum sensing signal molecule, is correlated with carbon metabolism in Bacillus amyloliquefaciens. In the present work, we found that mutation of srfA (ΔsrfA) led to an obviously changed carbon metabolism in B. amyloliquefaci...
Co-expressing Eranthis hyemalis lysophosphatidic acid acyltransferase 2 and elongase improves two very long chain polyunsaturated fatty acid production in Brassica carinata [0.03%]
冬凌草LPA酰基转移酶2和延长酶的共表达提高二月兰中两种非常长链多不饱和脂肪酸的产量
Dauenpen Meesapyodsuk,Yi Chen,Shengjian Ye et al.
Dauenpen Meesapyodsuk et al.
Docosadienoic acid (DDA, 22:2-13,16) and docosatrienoic acid (DTA, 22:3-13,16,19) are two very long chain polyunsaturated fatty acids (VLCPUFAs) that are recently shown to possess strong anti-inflammatory and antitumor properties. An ELO ty...
Synthetic production of prenylated naringenins in yeast using promiscuous microbial prenyltransferases [0.03%]
利用兼性微生物前药转移酶在酵母中合成香叶基化的柚皮素类化合物
Shota Isogai,Nobuyuki Okahashi,Ririka Asama et al.
Shota Isogai et al.
Reconstitution of prenylflavonoids using the flavonoid biosynthetic pathway and prenyltransferases (PTs) in microbes can be a promising attractive alternative to plant-based production or chemical synthesis. Here, we demonstrate that promis...
Analysis of metabolic network disruption in engineered microbial hosts due to enzyme promiscuity [0.03%]
酶多功能性导致工程宿主代谢网络破坏的分析
Vladimir Porokhin,Sara A Amin,Trevor B Nicks et al.
Vladimir Porokhin et al.
Increasing understanding of metabolic and regulatory networks underlying microbial physiology has enabled creation of progressively more complex synthetic biological systems for biochemical, biomedical, agricultural, and environmental appli...
Development of antisense RNA-mediated quantifiable inhibition for metabolic regulation [0.03%]
开发用于代谢调控的可定量抑制反义RNA技术
Ruihua Zhang,Yan Zhang,Jian Wang et al.
Ruihua Zhang et al.
Trans-regulating elements such as noncoding RNAs are crucial in modifying cells, and has shown broad application in synthetic biology, metabolic engineering and RNA therapies. Although effective, titration of the regulatory levels of such e...