Heterologous expression of MlcE in Saccharomyces cerevisiae provides resistance to natural and semi-synthetic statins [0.03%]
异源表达MlcE使酿酒酵母获得天然和半合成他汀类药物的抗性
Ana Ley,Hilde Cornelijne Coumou,Rasmus John Normand Frandsen
Ana Ley
Statins are inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase, the key enzyme in cholesterol biosynthesis. Their extensive use in treatment and prevention of cardiovascular diseases placed statins among the best selling drugs. C...
Benchmarking two commonly used Saccharomyces cerevisiae strains for heterologous vanillin-β-glucoside production [0.03%]
评估两种常用的酿酒酵母菌株异源生产香草酸-β-d-葡糖苷的性能
Tomas Strucko,Olivera Magdenoska,Uffe H Mortensen
Tomas Strucko
The yeast Saccharomyces cerevisiae is a widely used eukaryotic model organism and a key cell factory for production of biofuels and wide range of chemicals. From the broad palette of available yeast strains, the most popular are those deriv...
Co-evolution of strain design methods based on flux balance and elementary mode analysis [0.03%]
基于通量平衡和基元模式分析的菌株设计方法的协同进化
Daniel Machado,Markus J Herrgård
Daniel Machado
More than a decade ago, the first genome-scale metabolic models for two of the most relevant microbes for biotechnology applications, Escherichia coli and Saccaromyces cerevisiae, were published. Shortly after followed the publication of Op...
Analyzing redox balance in a synthetic yeast platform to improve utilization of brown macroalgae as feedstock [0.03%]
分析合成酵母平台中的氧化还原平衡以改善褐藻原料的利用
C A Contador,C Shene,A Olivera et al.
C A Contador et al.
Macroalgae have high potential to be an efficient, and sustainable feedstock for the production of biofuels and other more valuable chemicals. Attempts have been made to enable the co-fermentation of alginate and mannitol by Saccharomyces c...
Effect of pyruvate kinase gene deletion on the physiology of Corynebacterium glutamicum ATCC13032 under biotin-sufficient non-glutamate-producing conditions: Enhanced biomass production [0.03%]
丙酮酸激酶基因敲除对赖氨酸棒杆菌ATCC13032生理特性的影响：生物素非限制及不产生谷氨酸条件下的生物量增强作用
Kazunori Sawada,Masaru Wada,Takuya Hagiwara et al.
Kazunori Sawada et al.
The effect of pyruvate kinase gene (pyk) deletion on the physiology of Corynebacterium glutamicum ATCC13032 was investigated under biotin-sufficient, non-glutamate-producing conditions. In a complex medium containing 100 g/L glucose, a defi...
Engineering the fatty acid metabolic pathway in Saccharomyces cerevisiae for advanced biofuel production [0.03%]
利用基因工程技术改造酿酒酵母脂肪酸代谢途径生产生物燃料的研究
Xiaoling Tang,Jaslyn Lee,Wei Ning Chen
Xiaoling Tang
Fatty acid-derived fuels and chemicals have attracted a great deal of attention in recent decades, due to their following properties of high compatibility to gasoline-based fuels and existing infrastructure for their direct utilization, sto...
Dynamic metabolic flux analysis using B-splines to study the effects of temperature shift on CHO cell metabolism [0.03%]
基于B样条的动态代谢流分析及其在研究温度变化对CHO细胞代谢影响中的应用
Verónica S Martínez,Maria Buchsteiner,Peter Gray et al.
Verónica S Martínez et al.
Metabolic flux analysis (MFA) is widely used to estimate intracellular fluxes. Conventional MFA, however, is limited to continuous cultures and the mid-exponential growth phase of batch cultures. Dynamic MFA (DMFA) has emerged to characteri...
Shawn Pugh,Rebekah McKenna,Ibrahim Halloum et al.
Shawn Pugh et al.
Benzyl alcohol is an aromatic hydrocarbon used as a solvent and an intermediate chemical in the pharmaceutical, cosmetics, and flavor/fragrance industries. The de novo biosynthesis of benzyl alcohol directly from renewable glucose was herei...
The LASER database: Formalizing design rules for metabolic engineering [0.03%]
LASER数据库：代谢工程设计规则的规范化
James D Winkler,Andrea L Halweg-Edwards,Ryan T Gill
James D Winkler
The ability of metabolic engineers to conceptualize, implement, and evaluate strain designs has dramatically increased in the last decade. Unlike other engineering fields, no centralized, open-access, and easily searched repository exists f...
Daniel G Olson,Marybeth Maloney,Anthony A Lanahan et al.
Daniel G Olson et al.
A key tool for metabolic engineering is the ability to express heterologous genes. One obstacle to gene expression in non-model organisms, and especially in relatively uncharacterized bacteria, is the lack of well-characterized promoters. H...