Dynamic 3D Chromatin Reorganization during Establishment and Maintenance of Pluripotency [0.03%]
pluripotency建立和维持过程中的动态三维染色质重构
Bobbie Pelham-Webb,Dylan Murphy,Effie Apostolou
Bobbie Pelham-Webb
Higher-order chromatin structure is tightly linked to gene expression and therefore cell identity. In recent years, the chromatin landscape of pluripotent stem cells has become better characterized, and unique features at various architectu...
A Human Retinal Pigment Epithelium-Based Screening Platform Reveals Inducers of Photoreceptor Outer Segments Phagocytosis [0.03%]
基于人视网膜色素上皮细胞的筛选平台发现诱导光受体外节吞噬作用的化合物
Sven Schreiter,Katerina Vafia,Rico Barsacchi et al.
Sven Schreiter et al.
Phagocytosis is a key function in various cells throughout the body. A deficiency in photoreceptor outer segment (POS) phagocytosis by the retinal pigment epithelium (RPE) causes vision loss in inherited retinal diseases and possibly age-re...
Unique Functional Properties of Mature Adult-Born Neurons in the Mouse Olfactory Bulb [0.03%]
小鼠嗅球成熟的新产生的神经元的独特功能属性
Natalie Fomin-Thunemann,Yury Kovalchuk,Stefan Fink et al.
Natalie Fomin-Thunemann et al.
The rodent olfactory bulb (OB) is continuously supplied with adult-born cells maturing into GABAergic neurons. Using in vivo ratiometric Ca2+ imaging to readout ongoing and sensory-driven activity, we asked whether mature adult-born cells (...
Chromatin-Associated Membraneless Organelles in Regulation of Cellular Differentiation [0.03%]
染色质相关无膜细胞器在调控细胞分化中的作用
Markus Grosch,Sebastian Ittermann,Dmitry Shaposhnikov et al.
Markus Grosch et al.
Membrane-free intracellular biocondensates are enclosures of proteins and nucleic acids that form by phase separation. Extensive ensembles of nuclear "membraneless organelles" indicate their involvement in genome regulation. Indeed, nuclear...
An In Vitro Differentiation Protocol for Human Embryonic Bipotential Gonad and Testis Cell Development [0.03%]
人类胚胎生殖腺和睾丸细胞发育的体外分化方案
Ingrid M Knarston,Svenja Pachernegg,Gorjana Robevska et al.
Ingrid M Knarston et al.
Currently an in vitro model that fully recapitulates the human embryonic gonad is lacking. Here we describe a fully defined feeder-free protocol to generate early testis-like cells with the ability to be cultured as an organoid, from human ...
Insulin Promotes Mitochondrial Respiration and Survival through PI3K/AKT/GSK3 Pathway in Human Embryonic Stem Cells [0.03%]
胰岛素通过PI3K / AKT / GSK3途径促进人胚胎干细胞的线粒体呼吸和存活
Zhili Ren,Hui Zhong,Chengcheng Song et al.
Zhili Ren et al.
Insulin is an essential growth factor for the survival and self-renewal of human embryonic stem cells (hESCs). Although it is best known as the principal hormone promoting glycolysis in somatic cells, insulin's roles in hESC energy metaboli...
β-Globin Lentiviral Vectors Have Reduced Titers due to Incomplete Vector RNA Genomes and Lowered Virion Production [0.03%]
完整的β-珠蛋白 lentiviral 转移载体RNA缺陷导致载体滴度下降及病毒体生成减少
Jiaying Han,Kevin Tam,Feiyang Ma et al.
Jiaying Han et al.
Lentiviral vectors (LVs) commonly used for the treatment of hemoglobinopathies often have low titers and sub-optimal gene transfer efficiency for human hematopoietic stem and progenitor cells (HSPCs), hindering clinical translation and comm...
Qijing Lei,Xin Lai,Jitske Eliveld et al.
Qijing Lei et al.
In vitro spermatogenesis has been achieved by culturing mouse embryonic stem cells (ESCs) together with a cell suspension of male juvenile gonad. However, for human fertility treatment or preservation, patient-specific ESCs or juvenile gona...
Isogenic Sets of hiPSC-CMs Harboring Distinct KCNH2 Mutations Differ Functionally and in Susceptibility to Drug-Induced Arrhythmias [0.03%]
携带有不同KCNH2突变的诱导多能干细胞心室肌细胞等基因组集合在功能和由药物诱发的心律失常敏感性方面具有差异性
Karina O Brandão,Lettine van den Brink,Duncan C Miller et al.
Karina O Brandão et al.
Mutations in KCNH2 can lead to long QT syndrome type 2. Variable disease manifestation observed with this channelopathy is associated with the location and type of mutation within the protein, complicating efforts to predict patient risk. H...
Bipotent Progenitors Do Not Require Androgen Receptor for Luminal Specification during Prostate Organogenesis [0.03%]
双能祖细胞不需要雄激素受体在前列腺发生过程中进行管腔分化指定
Maho Shibata,Nusrat J Epsi,Shouhong Xuan et al.
Maho Shibata et al.
Androgen receptor (AR) plays a fundamental role in most aspects of adult prostate homeostasis, and anti-androgen therapy represents the cornerstone of prostate cancer treatment. However, early prostate organogenesis takes place during pre-p...